摘要
目的 应用图像分析和免疫组织化学技术观察和分析兔坐骨神经高速弹丸震荡伤后腰髓背根神经节病理改变及意义。方法 大耳白兔 2 5只 (包括正常对照 5只 ) ,致伤靶点为右后肢外侧坐骨神经体表投影线中点 (0 38g钢珠 ,0 6 5g装药量 ) ,观察伤后 1、3、7、14d(n =5 )腰髓背根神经节病理改变及一氧化氮合酶 (nitricoxidesynthase ,NOS)表达变化 (免疫组化法 ) ,并进行神经元计数及神经元截面积图像分析。结果 腰髓背根神经节伤后发生出血、水肿、神经元皱缩、坏死等变化 ,伤后 3dNOS表达显著增强 ,伤后 7d神经元数显著减少 ,神经元平均截面积显著减少。结论 坐骨神经高速弹丸震荡伤后腰髓背根神经节发生了较重的损伤。
Objective To study the pathological changes of dorsal root ganglion of the lumbar spinal cord in the rabbit after right sciatic nerve injury by high velocity projectile with the technology of immunohistochemistry staining and image analyzing. Methods 25 rabbits were used in this experiment. The target was the midpoint of the lateral body surface projection of the right sciatic nerve in rabbit. The missile projectile was 0.38 g ball with 0.65 g charge. The following pathological changes and the expression of NOS (1st,3rd,7th,14th day) of dorsal root ganglion of the lumbar spinal cord were found under microscope. The number of neurons and the area of neuron were counted also. Results Hemorrhage,edema,shrinkage and necrosis of neurons were observed,and the expression of NOS increased significantly in the 3rd day. The number of neurons and the area of neuron decreased in the 7th day. Conclusion The pathological changes of dorsal root ganglion of the lumbar spinal cord in the rabbit after right sciatic nerve injury by high velocity projectile were severe.
出处
《中国急救医学》
CAS
CSCD
北大核心
2004年第3期171-172,共2页
Chinese Journal of Critical Care Medicine
基金
国家重点基础研究发展规划专项经费资助项目 (G19990 5 42 0 6)
关键词
坐骨神经
背根神经节
免疫组化
Sciatic nerve
Dorsal root ganglion
Immunohistochemistry
