摘要
目的 探讨黄连素对结肠癌细胞系HT 2 9的作用及其相关机制 ,为阐明黄连素作为一种新的结肠癌化学治疗药物进行理论上的准备并提供相关实验结果。方法 黄连素 0 1、0 3、3 0、30 0 μmol/L加入到HT 2 9结肠癌细胞系培养液中 ,每天测量各有关值。同时以NS 398为环氧合酶(COX 2 )活性抑制剂 ,对比观察黄连素对COX 2活性的影响。采用氮蓝四唑盐实验法检测细胞的生长和增殖 ,逆转录 PCR法检测COX 2mRNA ,免疫细胞化学法检测COX 2蛋白表达 ,ELISA法检测前列腺素 (PG)E2 的含量。结果 黄连素在浓度大于 0 3μmol/L时则有明显的量效关系。黄连素在浓度大于 0 3μmol/L时对COX 2mRNA水平和蛋白的表达有明显的抑制作用。黄连素对PGE2 的生成有显著抑制作用。结论 黄连素抑制COX 2在mRNA水平和蛋白质水平的表达 ,同时也抑制COX 2活性 ,进而抑制PGE2 的生成 ,这可能为黄连素抑制HT 2 9细胞生长和增殖的机制之一。
Objective To investigate the effect of berberine on human colon cancer cell line HT 29 and the mechanisms of the effects.Methods Add 0 1、0 3、3 0、30 0 μmol/L berberine into the HT 29 cell culture media The related values were recorded everyday. NS 398 was used as a cyclooxygenase 2 (COX 2) enzyme activity inhibiting agent. The related values included cell growth, test of COX 2 mRNA using RT PCR method, expression of COX 2 protein with immunocytochemical method, and concentration of prostaglandin E 2 with ELISA method. Results When the concentration of berberine was higher than 0 3 μmol/L, there was a significant dose dependent effect When the berberine concentration was higher than 0 3 μmol/L, berberine could inhibit the COX 2 in mRNA and protein level It could decrease the concentration of prostaglandin E 2 in the culture media Conclusions Berberine can inhibit COX 2 in mRNA and protein level It can also inhibit the activity of COX 2. This may be one of the mechanisms that berberine inhibit the growth and proliferation of HT 29 human colon cell line.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2003年第8期558-560,共3页
Chinese Journal of Internal Medicine
基金
国家自然科学基金资助项目 (3 9970 90 4)
