摘要
目的 研究竹叶抗氧化物的致畸性和致突变性。方法 设 1 43、 2 87、 4 3 0g/kg体重 3剂量组 ,进行传统致畸研究 ;设 8、 40、 2 0 0、 10 0 0和 5 0 0 0 μg/皿 5个剂量组 ,进行Ames试验 :设 2 5、 5 0和 10 0g/kg体重 3个剂量组 ,观察其对小鼠骨髓细胞微核和小鼠精子畸形的影响。结果 3剂量组各项指标均未见有明显的母体毒性和胚胎毒性、致畸性。各剂量组在体内体外致突变性研究中未见有致突变作用。结论 在本实验条件下 ,竹叶抗氧化物无致畸性和致突变性。
Objective To study the teratogenicity and mutagenicity of the antioxidant of bamboo leaves for its safety assessment.Methods A traditional teratogenic assay was carried out by setting 3 different dose groups with 1.43, 2.87, 4.30g/kg b.w.in rats. And Salmonella typhimurium/mammals micorsomal enzyme test was processed by setting 5 different dose groups with 8, 40, 200, 1000 and 5000μg per plate. Bone marrow cell micronucleus test and sperm abnormality test in mice were processed by setting 3 different dose groups with 2.5,5.0 and 10.0g/kg b.w. Results There were no obvious maternal toxicity, embryotoxicity and teratogenicity in the 3 groups,and there were also no obvious mutagenicity observed in vitro and vivo test. Conclusions The study shows that the antioxidant of bamboo leaves may have no teratogenicity and mutagenicity.
出处
《浙江预防医学》
2004年第3期15-18,共4页
Zhejiang Journal of Preventive Medicine
