摘要
目的 探讨白细胞在早期糖尿病大鼠视网膜毛细血管中粘附、堆积及其与视网膜微血管形态学变化的关系。 方法 健康成年雄性 Wistar大鼠 90只 ,随机分成正常对照组和链脲佐菌素 (strepto-zotocin,STZ)诱导的糖尿病组各 4 5只 (3、7、14 d组各 5只 ,30、90、180 d组各 10只 )。取大鼠右眼制备视网膜消化铺片 ,进行形态学观察及白细胞共有抗原 (leukocyte common antigen,CD4 5 )单克隆抗体免疫组织化学研究。 结果 正常对照组视网膜毛细血管中有少许 CD4 5阳性细胞 ;糖尿病发生 3d后 ,CD4 5的表达明显增加 ,病程第 14 d达高峰 ,以后基本稳定。病程 90 d时 ,视网膜毛细血管已出现节段性膨大、囊样扩张及闭锁等形态学改变 ;病程 180 d时 ,上述病变进一步加重。 结论 白细胞粘附发生在糖尿病视网膜病变 (diabetic retinopathy,DR)的早期阶段 ,是 DR在微血管水平病理变化的开端。白细胞粘附可能作为视网膜微血管形态学改变的基础 ,在 DR的发生发展中起着重要作用。
Objective To observe the adhension and stracking of leukocyte in the capillary vessels, and investigate the relationship between leukocyte and microvascular morphologic changes in retinal microvessels of rats with early diabetes. Methods A total of 90 healthy adult male Wistar rats were randomly divided into control and diabetes (induced by Streptozotocin, STZ) groups with 45 rats in each group. The rats in the diabetic group were further divided into 3, 7, and 14 days groups with 5 rats in each group, and 30, 90, and 180 days groups with 10 rats in each group. The right eyes of rats in each group were prepared for retinal digest preparations. The expression of leukocyte common antigen (CD45) was detected by immunohistochemical staining. Results Few CD45 positive cells in the retinal capillaries were seen in the control group. The expression of CD45 was significantly increased in the retinal capillaries 3 days after diabetes induction, and reached a peak at the 14th day. Morphological changes including capillary telangiectasia, atresia, and irregularity of capillary caliber were found in the retinal capillaries of rats 90 days after diabetes induction. The changes were aggravated 180 days after diabetes induction. Conclusion Leukocyte adhesion occurs in the early stage of diabetic retinopathy (DR), and is the beginning of the microvascular pathological changes. Leukocyte adhesion may play an important role in the occurrence and development of DR as the foundation of microvascular morphological changes.
出处
《中华眼底病杂志》
CAS
CSCD
2003年第6期344-347,共4页
Chinese Journal of Ocular Fundus Diseases
基金
"广东省眼科视觉科学重点实验室"专项基金资助项目(B60 1 0 5)
