摘要
目的 探讨慢性前列腺炎疼痛的神经机制以及疼痛产生与盆底肌功能障碍的关系。方法 用荧光素逆行双标法和免疫组化方法进行研究 ,实验组将荧光素碘化丙啶 (PropidiumIodide ,PI)注入前列腺 ,双苯甲亚胺 (Bisbenzimide ,Bb)注入肛门外括约肌、生殖股神经、股外侧皮神经、髂腹股沟、股神经、髂腹下神经 ;此后在有双标细胞的切片上行免疫组化检查。结果 在L1 ～L2 和L6 ～S2 的后根神经节 (Dorsalrootganglia ,DRG)中发现荧光素双标细胞 ,对照无双标细胞 ;部分双标细胞含降钙素基因相关肽 (Calcitoningene relatedpeptide ,CGRP)和P物质 (Psubstance ,SP)。结论 前列腺炎引起的会阴部牵涉痛的发生机制可能与发生在DRG水平的轴突反射以及由此引起的神经源性炎症有关 ,并与盆底肌功能障碍密切相关。
Objective To explore the mechanism of the referred pains in pelvic pain due to chronic prostatitis and the relation between pain due to prostatitis and pelvic floor muscle dysfunction. Methods The retrograde fluorescent double labeling and immunohistochemistry were used. A total of 24 Wistar rats were divided two groups, 12 in each group. In the first group, Propidium Iodide(PI) was injected into the prostate, Bisbenzimide(Bb)into the external anal sphincter. In the second group, PI was injected into the prostate, Bb into genitofemoral, iliohypogastric, ilioinguinal, femoral and lateral femoral cutaneous nerves. Results The fluorescent double labeled cells were found in the L 1 L 2 and L 6～S 3 dorsal root ganglia(DRG) and some of them were confirmed to contain calcitonin gene related peptide(CGRP) and P substance (SP) by immunohistochemistry. Conclusion The findings confirm that the peripheral process of DRG cells dichotomizes into prostate, external anal sphincter and somatic parties, and some of these cells contain CGRP and SP, which indicates the referred pains in pelvic floor may be caused by axon reflex in the peripheral process of DRG cells. Meanwhile, neurogenic inflammation might play an important role in this persistent pain status. Pain due to prostatitis is correlated with pelvic floor muscle dysfunction.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第15期1358-1360,共3页
Journal of Third Military Medical University
基金
重庆市应用基础研究资助项目 ( 2 0 0 2 )
