摘要
【目的】观察复方肝毒清的体内外抗乙肝病毒作用。【方法】体内实验采用先天感染鸭乙肝模型,检测用药前后鸭血清乙肝病毒脱氧核糖核酸(DHBV DNA)的动态变化;体外实验采用2.2.15细胞为模型,检测用药后复方肝毒清对细胞培养上清中乙型肝炎表面抗原(HBsAg)和乙型肝炎e抗原(HBeAg)的抑制作用。【结果】体内实验:给药第10天时血清DHBVDNA滴度显著下降(与模型组比较P<0.01).而停药第3天后又有回升,但仍低于模型组(P<0.01);体外实验:复方肝毒清对细胞分泌HBsAg的治疗指数(50％毒性浓度:50％抑制浓度)为66.81,对分泌HBeAg的治疗指数为34.18。【结论】复方肝毒清在体内外均具有抗乙肝病毒作用。
[Objective] To observe the in-vitro and in-vivo inhibitory effect of compound Ganduqing, a Chinese herbal prescription, on hepatitis B virus (HBV). [Methods] Duck models of congenital infection of HBV was used in the invivo test and the dynamic changes of serum duck HBV DNA titer was observed. 2.2.15 cell line was applied in the invitro test and the inhibitory effect of Ganduqing on HBsAg and HBeAg was evaluated. [Results] In the in-vivo test, serum duck HBV DNA titer was obviously decreased after 10 days of administration (P<0.01) and began to rebound after 3 days of suspension but still lower than that in model group (P<0.01). In the in-vitro test, the therapeutic index (the ratio of 50% toxic concentration and 50% inhibitory concentration) of Ganduqing was 66.81 for HBsAg and 34.18 for HBeAg. [Conclusion] Compound Ganduqing exerts a certain in-vitro and in-vivo inhibitory effect on HBV.
出处
《广州中医药大学学报》
CAS
2004年第2期134-136,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
关键词
复方肝毒清
乙型肝炎病毒
抑制作用
体外实验
乙型肝炎表面抗原
HEPATITIS B VIRUS, DUCK/drug effects
HEPATITIS B SURFACE ANTIGENS
HEPATITIS B E ANTIGENS
COMPOUND GANDUQING/therapeutic use
HEPATITIS B/TCD therapy
ACYCLOVIR/therapeutic use
