摘要
以适宜西北地区种植的优质牧草冰草属(AgropyronGaertn)中的三个不同种———蒙古冰草新品系(A.mongolicumKeng)、航道冰草(A.cristatumcv.Fairway)、诺丹冰草(A.desertorumcv.Nordan)和一个种间杂种冰草———蒙农杂种冰草(A.cristatum×A.desertorumcv.Mengnong)为材料,分别以幼穗为外植体建立了冰草组织培养再生体系。诱导愈伤组织的培养基为改良MS+2,4 D2.0mg/L,分化培养基为MS(无附加成分),在1/2MS培养基上生根后得到完整小植株。结果表明在本试验条件下,不同长度的幼穗在培养时,其愈伤组织发生的部位及其增殖速度不同;4种材料愈伤组织诱导率和分化率无明显差异,均可有效诱导愈伤组织并分化成再生植株,再生植株的产生主要通过直接器官发生途径。
Plant regeneration from immature inflorescence through direct callus induction was investigated in the case of different species of wheatgrass(Agropyron Gaertn -A.mongolicum Keng,A.cristatum cv.Fair- way,A.desertorum cv.Nordan.)and one of a hybrid wheatgrass(A.cristatum × A.desertorum cv.Mengnong).Explants were directly cultured on advanced MS solid medium with 2.0 mg/L 2,4-D to induce calli which were then transferred to hormone free MS medium for callus differentiation and 1/2MS medium for rooting.There were apparent differences in response of callus induction and proliferation to immature inflorescence ranging in different lengths.The result showed no obvious differences in callus initiation frequency and plant regeneration frequency among four materials.The plant regeneration occurred mainly by means of direct organogenesis.
出处
《华北农学报》
CSCD
北大核心
2004年第1期17-20,共4页
Acta Agriculturae Boreali-Sinica
基金
国家转基因植物研究与产业化专项资助项目(J2002-B-008)
关键词
冰草属植物
组织培养
再生体系
外植体
培养基
Wheatgrass(Agropyron Gaertn)
Immature inflorescence culture
Plants regeneration
