摘要
目的 研究基质金属蛋白酶 -9(MMP -9)、基质金属蛋白酶组织抑制因子 -1(TIMP-1)在慢性肝病患者肝纤维化过程中的表达和动态变化。方法 用酶标法检测75例慢性乙型病毒性肝炎和40例乙肝后肝硬化患者血清中MMP -9和TIMP -1的浓度。结果血清MMP-9和TIMP -1浓度在慢性肝病患者肝纤维化过程中逐渐升高 ,与正常对照组比较有显著性意义 (P<0.01) ;其中轻度患者的MMP-9与中度、重度、肝硬化之间 ,肝硬化与中度、重度之间两两比较有显著性差异 (P<0.05或P<0.01) ;除了慢性乙肝重度患者的TIMP -1与肝硬化之间比较无意义外 ,其余各组之间两两比较均有显著性差异 (P<0.01)。结论 血清MMP -9和TIMP -1在慢性肝病患者肝纤维化过程中可能起促进作用 ,能够被用来做为判断肝纤维化程度的指标。
Objective To investigate the dynamic change and expression of Matrix Metalloproteinase-9(MMP-9)and tissue inhibitor metalloproteinase-1(TIMP-1)in patients with chronic hepatiecs B(CHB)and liver cirrhosis(LC). Methods serum MMP-9and TIMP-1were deˉtected by ELISA in 75 patients with CHB and 40 patients with LC and20healthy people. Results (1)serum MMP-9and TIMP-1leveles increased in CHB and LC.there were remarkably significant between patients with chronic liver disease and control group(p<0.01).MMP-9levels in severe degrees of CHB and LC were higher than in mild and moderate of CHB(p<0.05/0.01).and serumMMP-9levels in LC were notably higher than in severe degrees of CHB too.(2)seurm TIMP-1 increased successively in mild,moderate and severe degrees of CHB and LC(p<0.01),besides there were no significiant difference between two groups in severe degrees of CHB and LC(p>0.05). Conclution serum MMP-9 and TIMP-1 leveles increase in CHB and LC.They might participate in the development of hepatic fibrosis and can be used as diagnostic indexes for hepatic fibrosis in chronic liver diseases.
出处
《浙江临床医学》
2004年第3期183-184,共2页
Zhejiang Clinical Medical Journal
