摘要
采用 75MeV u16O8+ 离子 (降能至 36MeV u ,使其在胚乳中的射程约为 2 4mm ) ,从种子顶部垂直注入胚乳 ,通过“嫁接”技术后进行萌发。处理后观测结果表明 ,当代根尖细胞染色体的微核率和畸变率与注入剂量呈正相关 ;测定了当代抗氧化酶活性、丙二醛 (MDA)含量和蛋白质含量的变化 ;经多代大田培育获得了矮杆与各种穗型的变异 ,且具有遗传性。结果显示离子注入胚乳不仅对生物的修复系统有较大影响 ,而且能够诱发后代突变。
MeV/u 16 O 8+ ions (degraded to 36MeV/u) were used to implant into endosperm about 2.4mm on top of the seeds. Germination started after a `grafting' technique was employed. Chromosomal aberration frequency and micronucleus frequency of the root-tip cells in M 0 were measured. The results indicate that the frequencies were proportional to implanted dose. Antioxidant enzyme activity, MDA content and protein content of present generation M 0 were assayed. Farm culture was carried out in many generations. Short-stem and various variation of ear-type were obtained and the variation possess heredity. It showed that the endosperm implanted by the ions not only affected biological repair system, but also induced the mutation of offspring.
出处
《核农学报》
CAS
CSCD
北大核心
2004年第2期93-96,共4页
Journal of Nuclear Agricultural Sciences
基金
甘肃省自然科学基金 (SJ970 60 7)
中国科学院寒区旱区环境与工程研究所创新项目 ( 2 10 0 69)
