摘要
目的 了解手术过程中手术室空气微生物动态变化及其对医院感染的影响。方法 选择 5所医院的手术室 ,于春夏秋冬 4个季节 ,分别在手术室消毒后使用前、患者进入手术室后、麻醉 消毒 铺单等准备阶段、手术开始后 1h和 2h、手术终末等 6个时段 ,采用沉降法进行空气采样。结果 春夏秋冬手术室空气微生物含量平均值和标准差为 :91 7.1 3± 5 78.73,92 2 .30± 5 94 .4 9,6 33.5 3± 2 96 .2 3,4 73.31± 2 0 5 .0 5。手术过程中各时段 (按方法中顺序 )空气微生物含量平均值和标准差分别为 :1 78.4 5± 1 0 3.1 2 ,5 0 1 .6 3± 1 5 2 .2 0 ,896 .0 5± 2 95 .5 3,6 31 .5 2±1 85 .87,973.5 1± 4 84 .5 2 ,1 2 38.32± 5 6 6 .2 8。结论 春夏季微生物含量较高。随着手术进程的开展 ,微生物含量呈上升趋势 ,当空气中微生物含量≥ 1 70 0CFU/m3 ,医院感染相关性增大。
Objective To evaluate the effect of microbial change in operating rooms on nosocomial infection. Methods Air samples of operating rooms in 5 hospitals were taken in four seasons, air precipitating method was used for taking air samples after the operating rooms were disinfected but before patients entered the operating rooms, patients entered operating rooms, operation preparation period, 1 hour and 2 hours after the beginning of operation and the end of operation,respectively. Results The microbial content in the operating rooms in spring, summer, autumn and winter were 917.13 ± 578.73 , 922.30 ± 594.49 , 633.53 ± 296.23 and 473.31 ± 205.05 , respectively, the microbial content in different periods of operation were 178.45 ± 103.12 , 501.63 ± 152.20 , 896.05 ± 295.53 , 631.52 ± 185.87 , 973.51 ± 484.52 and 1 238.32 ± 566.28 , respectively. Conclusion The microbial content in the air was high in the spring and summer, microbial content increases with the process of operation, when the microbial content reached at least 1 700 CFU/m 3, the potentiality of nosocomial infection will increase, the number of staff members in operating rooms and external environment can influence the microbial change in the air.
出处
《中国感染控制杂志》
CAS
2004年第2期161-162,145,共3页
Chinese Journal of Infection Control