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小鼠GITRL基因的克隆和序列分析 被引量：5

Cloning and Sequence Analysis of Mouse Glucocorticoid-induced Tumor Necrosis Factor Receptor Ligand Gene
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摘要 目的 :克隆小鼠GITRL基因全长编码区的cDNA ,同时对其序列分析。方法 :采用RT PCR方法 ,从小鼠树突状细胞获得GITRL基因的cDNA ,克隆至pMD18 T载体 ,选择阳性克隆并进行序列测定。结果 :扩增得到的GITRL基因编码区cDNA的全长 5 19bp ,编码 173个氨基酸残基 ,与GeneBank中发表的序列完全一致。结论 :获得小鼠GITRL基因的克隆 ,为进一步研究其生物学功能提供基础。 Objective: To clone and analyze the cDNA encoding mouse glucocorticoid-induced tumor necrosis factor receptor ligand (GITRL)gene, a type Ⅱ transmembrance protein of the tumor necrosis factor (TNF) superfamily. Methods: The cDNA of GITRL was amplified from total RNA extracted from mouse dendritic cells (DC) by RT-PCR and inserted into pMD18-T vector, and the sequence of the DNA was analyzed. Results: The cDNA of GITRL has the length of 519bp with an complete open reading frame, which encodes a product of 173 amino acid and shares 100% homology with the sequence of mRNA for mouse GITRL in GeneBank. Conclusion: The cDNA of GITRL was successfully cloned, which brought a foundation for further researching on its biological function.
作者 王胜军 马斌 仝佳 许化溪 杨胜利
机构地区 江苏大学医学技术学院免疫学研究室 江苏大学生命科学研究院
出处 《江苏大学学报（医学版）》 CAS 2004年第2期97-99,102,共4页 Journal of Jiangsu University：Medicine Edition
基金 国家自然科学基金资助项目 ( 3 0 3 0 0 169) 江苏省社会发展资助项目 (BS2 0 0 0 0 2 6)
关键词 GITRL基因 CDNA克隆 RT-PCR 调节性T细胞 小鼠 Glucocorticoid-induced tumor necrosis factor receptor ligand cDNA cloning RT-PCR Dendritic cell Mouse
分类号 R394 [医药卫生—医学遗传学]
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参考文献8

  • 1Nocentini G, Giunchi L, Ronchetti S, et al. A new member of the tumor necrosis factor/nerve growth factor receptor family inhibits T cell receptor-induced apoptosis [J]. Proc Natl Acad Sci USA, 1997, 94(12):6216-6221.
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  • 4Kime JD, Choi BK, Bae JS, et al. Cloning and characterization of GITR ligand [J]. Genes Immun, 2003, 4(8):564-569.
  • 5Sakaguchi S, Sakaguchi N, Asano M, et al. Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor α-chains(CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases[J]. J Immunol, 1995, 155(3):1151-1164.
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同被引文献48

  • 1王胜军,许化溪,钱晖,邵启祥,马斌,杨胜利.重组FoxP3腺相关病毒转染小鼠CD4^+CD25^-T细胞的实验研究[J].中国免疫学杂志,2006,22(4):291-293. 被引量：3
  • 2Tone M, Tone Y, Adams E et al. Mouse glucocorticoid-induced tumor necrosos factor receptor ligand is costimulatory for T cells [ J ]. PNAS USA, 2003 , 100(25) : 15059-15064.
  • 3Ji H B, Liao G, Faubion W A et al. Cutting edge: the nature ligand for glucocoritcoid-induced TNF receptor-related protein abrogates regulatory T cell suppression[J] .J Immunology, 2004, 172(10) :5823-5827.
  • 4Locksley R M, Killen N, Lenardo M J. The TNF and TNF receptor superfamilies: integrating mammalian biology[J]. Cell, 2001 , 104(4) :487-501.
  • 5Shimizu J,Yamazakis S,Takahashi T et al .Stimulation of CD25( + )CD4 ( + ) regulatory T cells through G1TR breaks immunological self-tolerance [J]. Nat Immunol,2(I)2,3 : 135-142.
  • 6Kim J D, Choi B K, Bae J S et ol. Cloning and characterization of GITR ligand [ J ]. Genes Immunol, 2003 , 4 (8) : 564-569.
  • 7Tamada K, and Chen L. Renewed interest in cancer immunotherapy with the tumor necrosis factor superfamily molecules[J]. Cancer Immtmol Immunother, 2006 , 55 ( 4 ) : 355 -362.
  • 8Tone M,Tone Y,Adams E,et al.Mouse glucocorticoid-induced tumor necrosos factor receptor ligand is costimulatory for T cells[J].PNAS USA,2003,100(25):15059-15064.
  • 9Ji HB,Liao G,Fanbion WA,et al.Cutting Edge:The nature ligand for glucocoritcoid-induced TNF receptor-related protein abrogates regulatory T cell suppression[J].J Immunol,2004,172(10):5823-5827.
  • 10Locksley RM,Killen N,Lenardo MJ.The TNF and TNF receptor superfamilies:integrating mammalian biology[J].Cell,2001,104(4):487-501.

引证文献5

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江苏大学学报（医学版）
2004年 第2期

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