摘要
目的 探讨Ca2 + CaM在M3 R介导逼尿肌细胞收缩中作用。方法 将原代培养逼尿肌细胞分为实验组和对照组 ,接种于 6孔培养板上培养 ,加入 10 -4mol/Lcarbachol和methoctramine ,采用Ca2 + 浓度和CaM活性检测试剂盒分别测定Ca2 + 浓度和CaM活性。结果 实验组中 ,[Ca2 + ]i和CaM的平均通道荧光对数值分别为 ( 3 2 6± 0 3 8)和 ( 2 87± 0 3 4) ,与对照组 [分别为 ( 2 0 6± 0 12 )、( 2 14± 0 2 4) ]相比 ,均有显著性的升高 (P <0 0 5 )。结论 Ca2 + CaM信号传导途径参与了M3
Objective To investigate the roles of Ca 2+ CaM in M3R mediated detrusor contraction Methods The primary cultured detrusor cells divided into experiment and control groups were inoculated in 6 hole plate, and then 10 -4 M carbachol and methoctramine were added The Ca 2+ concentrations and CaM activity were detected by Ca 2+ Test Kit and CaM Test Kit Results The Mean Channel Fluorescence values (lg) of Ca 2+ and CaM were (3 26±0 38) and (2 87±0 34) in the experiment group, respectively, and (2 06±0 12) and (2 14±0 24) in the control group, respectively Significant difference was found between the experiment group and the control group( P <0 05) Conclusion Ca 2+ CaM signal transduction pathway participates in the regulation of M3R mediated detrusor cell contraction
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第23期2123-2124,共2页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 39770 4 71)~~
