摘要
目的 建立赤丹退黄颗粒中芍药苷的含量测定方法。方法 高效液相色谱法 ,KromasilC1 8分析柱 ;乙腈 - 0 .1 %磷酸水溶液 (1 5∶85 )为流动相 ;检测波长 2 30nm。结果 芍药苷回归方程为Area =1 1 97.6 3×Amt.+2 1 .5 0 ,r =0 .9992 (n =6 ) ,在0 .0 5～ 2 .0 0 μg范围内呈线性关系 ,平均回收率为 1 0 2 .1 7% ,RSD为 1 .1 8%。结论 方法简便、准确、快速 ,可作为该制剂的定量方法。
Aim To establish an HPLC method for the determination of paeoniflorin in Chidan Tuihuang Keli.Method The paeoniflorin was separated on a Kromasil C 18 column at 230nm by ACN-H 2O with 0.1% H 3PO 4(15∶85) as a mobile phase.Result A linear relationship of paeoniflorin was good at 0.05μg to 2.00μg.The average recovery of paeoniflorin was 102.17% with RSD 1.18%.Conclusion The method is simple,accurate and reliable for the determination of paeoniflorin in Chidan Tuihuang Keli.
出处
《解放军药学学报》
CAS
2004年第2期134-136,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
