摘要
目的 :探讨鼻咽癌患者放射治疗后血浆EB病毒 (EBV)DNA水平与肿瘤复发的关系。方法 :11例临床缓解期患者和 9例临床复发患者分别抽血 3ml。所有的标本均采用荧光定量PCR的方法 ,在PE770 0型检测仪上定量检测血浆标本中EB病毒DNA的含量。结果 :肿瘤复发组 89% ( 8/ 9)的患者血浆中可检测到高拷贝数的EB病毒DNA ,中位浓度为4 70 0 0 0 (copies/ml) ,在临床缓解组患者中 ,仅 9% ( 1/ 11)的患者血浆中可检测到较高拷贝数的EB病毒DNA ,中位浓度为 0(copies/ml)。两组阳性率及中位浓度的比较均有显著性差异 (P <0 0 0 1)。结论 :鼻咽癌患者血浆EBVDNA水平与肿瘤复发关系密切 ,值得进一步研究。
Objective:To examine correlation between plasma EBV DNA and tumor recurrence in Nasopharyngeal carcinoma(NPC) patients after treatment. Methods:3ml blood sample was collected from thc every previously treated NPC patients with evidence of tumor recurrence or still in remission .All samples were detected by using fluorogenic quantitative PCR method in the PE Appliance Biosystems 7700 sequence Detector.Results:A high plasma EBV DNA level could be detected in 89%(8/9) patients with recurrence disease ,the median concentration was 470000(copies/ml).However ,in patients in continuous remission, only 9%(1/11) patients could be detected to have a high plasma EBV DNA level, the median concentration was 0(copies/ml)(P<0.001) .Conclusion:Plasma EBV DNA may be a valuable tool for diagnosing tumor recurrence in Nasopharyngeal carcinoma patients. Our results suggest that further investigation is warranted.
出处
《临床肿瘤学杂志》
CAS
2004年第2期122-125,共4页
Chinese Clinical Oncology
基金
中山大学附属肿瘤医院单病种基金资助
