摘要
以甜樱桃矮化砧木‘Gisela 5’和‘Gisela 6’的组培苗为试材,研究建立叶片的离体再生体系。结果表明,Gisela 6的再生能力高于Gisela 5;叶片正面接触培养基比背面接触培养基更有利于分化再生。用新梢顶端1-3节新展开的叶片制备外植体,分别接种在WPM+BA 7 mg/L+IBA 0.3 mg/L和WPM+BA 5 mg/L+IBA 0.5 mg/L的培养基上,黑暗处理15 d,Gisela 5的最高分化频率为75.1％,Gisela 6为100％。再生芽苗在三角瓶中生根容易,移入大田后生长正常。
An efficient and simple method for shoots regeneration from in vitro leaves of ' Gisela 5 ' and ' Gisela 6' (Prunus cerasus × P. canescens) was developed. Factors influencing in vitro leaf regeneration were studied. The ability to regenerate shoot from in vitro leaves of ' Gisela 6' was much greater than that of ' Gisela 5' ; Optimal results were obtained by culturing leaves adaxial side down on WPM medium containing BA 5 mg/L + IBA 0. 5 mg/L and BA 7 mg/L + IBA 0. 3 mg/L respectively in the dark for 15 days. Shoot regeneration rate of ' Gisela 6' reached up to 100% and that of ' Gisela 5 ' was 75. 1%. The regeneration shoot was easy to rooting and to transplant to the field.
出处
《园艺学报》
CAS
CSCD
北大核心
2004年第2期221-223,共3页
Acta Horticulturae Sinica
基金
山东省科技厅科技攻关项目(991010306)
山东省农业良种产业化工程项目(2000-3009)
