摘要
目的 应用木瓜蛋白酶雾化吸入和静脉注射法来复制兔肺气肿模型。方法 兔耳缘静脉注入 8%木瓜蛋白酶和让兔雾化吸入 5 %木瓜蛋白酶液制作模型。观测其动脉血气及肺功能并对肺组织进行病理学观察。结果 与正常组相比模型组兔动脉血pH值、PaO2 浓度降低 ,PaCO2 浓度升高 ;潮气量下降 ,呼吸总阻力、中心阻力、总气道阻力升高 ;病理组织切片光镜下见肺泡壁变薄、断裂 ,肺泡腔扩张、融合 ,典型肺气肿表现。结论 木瓜蛋白酶雾化吸入和静脉注射能成功复制出兔肺气肿模型。
Objective To establish proper animal model of emphysema by inhaling papain of ultrasonic atomization and injecting papain. Methods Rabbits were injected with 8% papain into auricle vena and inhaled 5% papain of ultrasonic atomization. Blood gas values and pulmonary function were examined. And the pulmonary tissues were investigated under microscope. Results Compared with normal group, the lung indications of pH, PaO 2, PaCO 2, Vt, Zrs, Rc, R(5Hz) in model group changed in evidence. The pathologic changes of pulmonary tissues accorded with emphysema's. Conclusion The rabbit model of emphysema was made with success, and it could meet all kinds of study requirement.
出处
《中国比较医学杂志》
CAS
2004年第2期108-111,I001,共5页
Chinese Journal of Comparative Medicine
