摘要
〔目的〕提高对艾滋病合并播散性荚膜组织胞浆菌病、马尔尼菲青霉菌病的实验诊断水平。〔方法〕取患者血液置于Bact alert12 0血培养仪配套培养瓶培养 ,然后转种哥伦比亚血平板、沙保劳琼脂平板 ,观察荚膜组织胞浆菌、马尔尼菲青霉菌分别于 2 5℃及 3 7℃孵育时的菌落特征、镜下形态特点。〔结果〕荚膜组织胞浆菌及马尔尼菲青霉菌均为双相型真菌 ,2 5℃培养为霉菌相。荚膜组织胞浆菌 ,无色素 ,生长缓慢 ,7-10d后可见白色绒毛样或棉花团样菌落 ,镜下可见特征性的齿轮状或棘状大分生孢子 ;3 7℃培养时为酵母相 ,镜下可见圆形、卵圆形 2 -4 μm的芽生孢子。马尔尼菲青霉菌 2 5℃培养 3 -4d为白色绒毛样菌落 ,菌落周围出现水溶性的葡萄酒样红色色素 ,镜下可见特征性的帚状枝 ;3 7℃孵育时为白色酵母样菌落 ,无色素 ,镜下可见圆形、卵圆形及特征性腊肠样孢子。〔结论〕艾滋病患者对荚膜组织胞浆菌、马尔尼菲青霉菌易感 ,易至播散性感染 ,从患者体内检出荚膜组织胞浆菌、马尔尼菲青霉菌是诊断荚膜组织胞浆菌病、马尔尼菲青霉菌病的金标准。
Objective To raise the level of experimental diagnosis of acute disseminated histoplasmosis capsulalum (HC) and penicillium marneffei (PM) secondary to AIDS.〔Methods〕 The blood samples were collected from patients and cultured in Bact/Alert 120 at 37℃ for 3-4 days and then inoculated on Columbia's blood agar plates Sabourand's agar plates at 25℃ and 37℃,respectively.The colony of the yeast and the mold were observed.Meanwhile,the features of HC and PM were observed under the microscope.〔Results〕Both HC and PM were dimorphic fungi.The HC grew slowly and had no pigment.It appeared to be mold at 25℃ and the macrocoridium cogwheel or thorn forms were found under the microscope.However,it appeared to be yeast at 37℃ and grew slower than in mold form.The gemmation spore was found under the microscope.PM appeared to be penicillium type at 25℃.A red pigment-like port wine was observed in the agar.Typical penicillus was found under microscope.It showed yeast type at 37℃ and no pigment,typical sausage-shaped spore were observed under the microscope.〔Conclusions〕 The patients with AIDS are easily infected by HC or PM.Detection of HC or PM from the patients is the golden standard for diagnosis of their infection.
出处
《中国卫生检验杂志》
CAS
2004年第2期135-137,共3页
Chinese Journal of Health Laboratory Technology
