摘要
目的探讨应用和拮抗血管内皮生长因子(VEGF)对骨折愈合相关因子表达的影响,并观察骨折愈合过程中的病理改变。方法用105只大耳白兔制作骨折模型,随机分为对照组、应用VEGF组和拮抗VEGF组,分别于伤后8、24、72h和1、3、5、8周测定各组动物骨折端相关因子的表达变化,同时取骨折端标本脱钙进行光镜观察。结果应用VEGF使骨折端对BMP的表达时间提前和延长,拮抗VEGF抑制了BMP的表达。应用VEGF组伤后3周时骨折端充填有纤维性骨痂、软骨骨痂和骨性骨痂,5周时新生骨以编织骨为主,8周时骨折正常愈合;拮抗VEGF导致伤后初期骨细胞坏死增多,1～5周各时相点骨折端均有灶性坏死出现,3周时骨折部位血管生成明显减少。结论骨折愈合过程需要多因子参与、协调,VEGF有可能是作为骨折愈合过程中的一种重要因子在发挥作用。
Objective To study the effect of application of vascular endoth el ial growth factor (VEGF) and anti-VEGF on the expression of fracture healing rel ated factors and observe the pathological changes at fractured site. Methods Fra cture models were established in 105 rabbits. All rabbits were randomly assigned into controlled group, VEGF treated group and anti-VEGF treated group. The fact ors related to fracture healing were detected, and pathological changes were obs erved at 8, 24, 72 h and 1, 3, 5, 8 weeks after fracture. Results After the appl ication of VGEF, BMP was expressed earlier and the express period was prolonged . On the contrary, anti-VEGF completely inhibited the expression of BMP in the f ractured ends. The fractured sites were filled with fibrous callus, cartilage ca llus and bony callus at the 3rd week and they were converted into woven bone at the 5th week. The fracture reached to normal healing at the 8th week in the VEGF treated group. Cellular necrosis increased in the earlier period in the VEGF po lyclonal antibody treated group, continuous focal necrosis was seen in the fract ured sites between 1st week to 5th week, vascularization reduced obviously at th e 3rd week. Conclusion Fracture healing is the result of mutual modulation and c oordination among many factors. VEGF may be an important factor which plays impo rtant role in the course of fracture healing.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
2003年第4期235-238,共4页
Chinese Journal of Orthopaedics
基金
国家重点基础研究发展规划项目(G1999054204)
关键词
血管内皮生长因子
骨折愈合
调控
动物模型
Fracture healing
Endothelial growth factors
Bone morphogenetic proteins
