摘要
目的 探讨创伤愈合过程中感觉神经肽P物质 (substanceP ,SP)对表皮干细胞迁移及NK -1受体表达的影响。 方法 将实验鼠分为SP组、辣椒素组和对照组 ,三组均致背部全层皮肤缺损 ,SP组致伤当天开始创面给予SP(1次 /d) ,辣椒素组致伤前 1周皮下预注射辣椒素 (cap saicin) ,利用核标记物BrdU作为表皮干细胞的示踪剂 ,观察三组创面愈合速度、NK -1受体表达情况和表皮干细胞的迁移特征。 结果 SP组创面愈合速度最快 ,创缘BrdU阳性细胞率最高 ,峰值出现最早 ;辣椒素组愈合速度最慢 ,BrdU阳性细胞率最低 ,峰值出现最晚 ;SP组除创缘外 ,在创面肉芽组织中也出现了BrdU阳性细胞 ,另两组未出现。三组表皮干细胞表面NK -1受体染色阳性。 结论 表皮干细胞存在NK -1受体。SP能明显加速创面愈合速度 。
Objective To study the effects of substance P (SP) o n migration of epidermal stem cells (ESC) and expression of neurokinin 1 (NK-1) r eceptor in wound healing. Methods The rats with full thickn ess skin injury on back were randomly divided into three groups, ie, SP group (i njected with SP at the day of injury, once per day), capsaicin group (subcutaneo usly injected with capsaicin before injury) and control group. BrdU, a nuclei ma rker, was used as the trace marker to observe wound healing, expression of NK-1 receptor and migration of ESC based on slow cell cycling and label restraining of ESC. Results Among three groups, the wound healing in the SP group was the fastest while that in the capsaicin group the slowest; the proportion of BrdU positive cells in the SP group ranked the highest while that in the capsaicin group the least. BrdU positive cells appeared in the granulatio n tissues in the SP group rather than in the other two groups. There could be se en positive staining of NK-1 receptors in the surface of ESC. Conclus ions NK-1 receptors exist in the epidermal stem cells. SP can not on ly obviously promote the wound healing and shorten the healing duration, but als o induce aggregation of ESC towards wound border or migration of ESC to granulat ion tissues.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2004年第3期142-145,共4页
Chinese Journal of Trauma
基金
国家重点基础研究发展规划资助项目(G19990 542 0 4 )
