摘要
目的 观察抗 EB(Epstein- Barr)病毒口服液对 EB病毒抗原表达的影响及对鼻咽癌 CNE2 细胞的细胞毒作用。方法 用间接荧光法测定抗 EB病毒口服液对 Raji细胞早期抗原 (early antigen,EA)表达及 B95- 8细胞病毒壳抗原 (virus capsid antigen,VCA)表达的影响 ;用 MTT法测定其对鼻咽癌 CNE2 细胞的细胞毒作用。结果 抗EB病毒口服液在无毒的浓度下 ,对 Raji细胞 EB病毒 EA抗原表达有较强的抑制作用 ,其 IC50 值为 0 .6 6 7m g/m L;对 B95- 8细胞 EB病毒 VCA抗原表达有较强的抑制作用 ,其 IC50 值为 0 .89mg/m L;对正丁酸钠激发的 B95- 8细胞 EB病毒 VCA抗原表达亦有较强的抑制作用 ,其 IC50 为 1.4 mg/m L。抗 EB病毒口服液对人鼻咽癌细胞 CNE2的 IC50 为 7.5 7mg/m L。结论 抗 EB病毒口服液能抑制 EB病毒抗原的表达 ,在较高的浓度下对鼻咽癌细胞CNE2 具细胞毒作用。
Object To study the inhibitory effect of Anti-EB Virus Liquor (AEVL) on EB (Epstein-Barr) virus antigen expression and its cytotoxicity. Methods The effect of AEVL on Raji cell early antigen (EA) expression and B 95-8 cell virus capsid antigen (VCA) expression was assayed by indirect fluorescent technique; the cytotoxicity on nasopharyngeal carcinoma CNE 2 cells was determined by MTT method. Results At the non-toxic concentration, AEVL had markedly inhibitory effect on Raji cell EB-virus, IC 50 was 0.667 mg/mL and showed powerfully inhibitory effect on B 95-8 cell EB-virus VCA expression, IC 50 was 0.89 mg/mL; and it had strongly inhibitory effect on B 95-8 cell EB virus VCA expression stimulated by sodium n-butyric acid, IC 50 was 1.4 mg/mL. The IC 50 of AEVL on human nasopharyngeal carcinoma CNE 2 cell was 7.57 mg/mL. Conclusion AEVL could inhibit EB virus antigen expression and have cytotoxicity on nasopharyngel carcinoma cells at high concentration.
出处
《中草药》
CAS
CSCD
北大核心
2004年第5期542-545,共4页
Chinese Traditional and Herbal Drugs
基金
国家"十五"科技攻关计划重大项目基金资助 ( 2 0 0 1BA70 1A0 7-10 )
中山大学重点学科课题基金联合资助 ( 980 96)
关键词
抗EB病毒口服液
EB病毒
抗原表达
鼻咽癌
Anti-EB Virus Liquor (AEVL)
EB virus
antigen expression
nasopharyngeal carcinoma
