摘要
目的 研究西沙某岛水域海水细菌的菌种、菌量和药敏情况。方法 对西沙某岛水域海水进行细菌学研究 ,按离岛远近海区共采集海水样本 80份 ,进行细菌学鉴定和药敏试验。结果 80份标本分离出 87株共 2 1种细菌 ,海水菌量平均为 337 4 4± 174 88cfu/ml,从海岸线由近而远菌量呈明显的梯级下降。溶藻弧菌检出率最高(4 5 98% ) ,离海岸线由近而远 ,检出率呈典型梯级上升 ;少动鞘氨醇单胞菌和莫拉菌属与溶藻弧菌相反 ,呈典型梯级下降 ;CDCgroupEO 2主要在近海水域检出 ,近海岸线和远海岸线水域均未检得。本次调查未检出霍乱弧菌、沙门菌、痢疾杆菌、大肠杆菌和金黄色葡萄球菌 ,16种抗生素对 4种主要检出菌均有很高的敏感度 ,对常用的四环素、庆大霉素、氯霉素和阿米卡星等均无耐药菌株。结论 西沙某岛水域海水洁净度好 ,主要菌种为溶藻弧菌、少动鞘氨醇单胞菌、莫拉菌属和土生丛毛单胞菌等 ,不同菌种在海水中的检出率呈明显的梯级变化 ;在远离海岸的海水中 77 2 7%为溶藻弧菌 ;
Objective To explore the bacterial species and total number of bacteria in seawater around an island in Xi-Sha archipelago and evaluate the drug sensitivity of the bacteria. Methods We studied the bacteria in various sea districts of the island. We collected 80 samples of seawater and cultured to identify bacterial species and their drug sensitivity. Results Vibrio ranked first on the isolation rate (45.98%), and from the near to distant sea districts, its isolation rate presented a typical stepwise upward tendency. The isolation rates of Sphingomonas paucimobilis and Moraxella species presented a typical stepwise downward tendency in contrast to Vibrio alginolyticus. CDC group EO-2 was mainly found in near sea districts but not along the seashore or in distant sea districts. The average bacteria number was 337.44±174.88 cfu/ml. The quantities of bacteria presented an obvious gradient downward trend from the near to distant sea districts. Vibrio cholerae, Samenella. dysenteridae, E.coli and Staphylococcus aureus were not detected. The four main kinds of bacteria found by us were all highly susceptible to 16 kinds of antibiotics, and we found no resistant strains to Tetracycline, Gentanycin. Chloromycin, and Amikacin. Conclusions The quality of seawater in Xi-Sha was fine in so far as bacterial contamination was concerned. Main bacterial species found included Vibrio alginolyticus, Sphingomonas paucimobilis, Moraxella species and Comamonas terrigena etc. The isolation rate of the different bacterial species presented obvious gradient variations. In sea districts far off the seashore, Vibrio alginolyticus accounted for 77.27% of the all bacteria. All the main bacterial species were highly sensitive to antibiotics.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第5期463-464,共2页
Medical Journal of Chinese People's Liberation Army
基金
军队医学科研"十五"计划指令性课题 (编号 0 1L0 55)
关键词
海水
细菌
数据收集
seawater
bacteria
data collection
