摘要
对重复片段PCR(rep PCR)基因指纹分析应用于链霉菌分子分型进行研究 ,结果表明rep PCR基因指纹分析具有分辨率高、稳定、重现性好、简便易行等特点 ,在一定程度上与 1 6SrDNA序列比较结果相一致 ,是一种快速而有效的DNA指纹技术 ,能反映出链霉菌种和菌株水平的基因型、系统发育和分类学关系 ,可应用于种及以下水平的分类和快速鉴定 。
Repetitive element PCR (rep PCR) genomic fingerprinting with the BOXA1R primer was investigated as a molecular typing tool for strains of Streptomyces. The results indicated that rep PCR DNA fingerprints of Streptomyces strains were relatively unique, simple, stable, and reproducible. To some extent, rep PCR generated genomic fingerprint analyses yield results that are in agreement with 16S rDNA sequences study. This method appears to reflect the genotypic, phylogenetic and taxonomic relationships of the genus Streptomyces in the species strain level. Therefore, it can be used as a rapid and efficient means of determining taxonomic diversity and phylogenetic structure, especially of large collections of strains.
出处
《微生物学报》
CAS
CSCD
北大核心
2004年第3期281-285,共5页
Acta Microbiologica Sinica
基金
北京理工大学基础研究基金资助项目 (BIT_UBF_2 0 0 3 0 6C0 8)~~
关键词
链霉菌
rep—PCR基因指纹分析
快速鉴定
Streptomyces, Repetitive-element PCR genomic fingerprinting, Rapid identification
