摘要
目的 观察重症急性胰腺炎 (SAP)时脑损伤与血清内皮素 (ET) 1/一氧化氮 (NO)比值的关系以及生长抑素和生长激素的保护作用。方法 80只大鼠经胰胆管逆行注射 3.5 %牛磺胆酸钠 2 .5ml/kg建立SAP模型 ,然后分成 4组 :重症胰腺炎组 (SAP组 ) ,生长抑素组 (S组 ,每天静脉注射 4 2μg/kg,共 2d) ,生长激素组 (G组 ,每天皮下注射 0 .5 μg/kg ,共 2d)和联合治疗组 ,每组 2 0只。另取 2 0只不造模大鼠为正常对照组。观察各组治疗前后脑水肿、血脑屏障、脑细胞凋亡变化以及与血清ET 1/NO的相关性。结果 SAP大鼠血清ET 1/NO显著升高 ,与脑水肿、血脑屏障损伤程度及脑细胞凋亡呈正相关。生长激素和生长抑素可降低血清ET 1/NO水平 ,减轻脑组织水肿 ,减少脑细胞凋亡 ,改善血脑屏障通透性。同时观察到SAP大鼠脑电图呈慢波改变 ,治疗后好转。结论 生长激素和生长抑素可降低SAP大鼠血清ET 1/NO比值 ,改善脑水肿和血脑屏障通透性 ,减少脑细胞凋亡 。
Objective To explore the protective effect of treatment of somatostatin combined with growth hormone on brain injury in early severe acute pancreatitis(SAP)rats, and to investigate the relationship between brain injury and ratio of endothelin 1(ET 1) /nitric oxide(NO). Methods SAP model was established by retrograde pancreatic bile duct injection of 3.5% sodium taurocholate at a dose of 2.5 ml/kg in rats. Eighty SAP rats were equally divided into SAP group, somatostatin (S) group (introvenous injection of somatostatin at a dose of 42 μg/kg once a day for 2 days), somatotropin (G) group (subcutaneous injection of somatotropin at a dose of 0.5 μg/kg once a day for 2 days) and S+G group. Twenty normal rats were used as controls. The changes of encephaledema and blood brain barrier permeability were measured by dry wet method and Evan′s blue staining, respectively. Apoptosis of brain cells was detected by TUNEL method, and the ratio of serum ET 1/NO was also determined. Results The ratio of serum ET 1/NO was remarkably increased in SAP rats, which was correlated with the intensity of brain edema, permeability of blood brain barrier and apoptosis of brain cells. The treatment of somatostatin combined with growth hormone reduced the ratio of ET 1/NO and thus decreased the intensity of encephaledema, the permeability of blood brain barrier and apoptosis of brain cells. The changes of the brain slow wave were found simultaneously by electroencephalography in SAP rats after the therapy. Conclusions The treatment of somatostatin combined with growth hormone in SAP rats can inhibit serum ET 1/NO, prevent the development of brain edema, decrease the permeability of blood brain barrier, and alleviate the brain cells apoptosis. All these result in the relief of the brain injury.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2004年第5期270-273,共4页
Chinese Journal of Digestion
