摘要
目的 :对食管癌及其癌前病变中Bcl- 2、MDM - 2和HPV - 16进行分析 ,以探讨食管癌发生原因及其机制。方法 :采用免疫组化方法 ,对正常食管上皮、食管癌及其癌前病变中Bcl - 2、MDM - 2蛋白的表达和HPV - 16进行对比观察。结果 :9例正常食管鳞状上皮中有 1例Bcl- 2染色阳性 (11 11% ) ,MDM - 2和HPV - 16全部阴性 ;39例食管癌中Bcl- 2、MDM - 2、HPV - 16染色分别有 33例 (84 6 1% )、2 5例 (6 4 10 % )和 13例 (33 33% )染色阳性 ;31例非典型增生中Bcl - 2、MDM - 2、HPV - 16染色分别有 7例 (2 2 5 8% )、11例 (35 4 8% )和 3例 (9 6 8% )染色阳性。三项指标在三组间比较均具有显著性差异 (P <0 0 5 ) ;食管癌HPV - 16阳性 (33 33% )与非典型增生 (9 6 8% )有显著性差异 (χ2 =4 14 ,P <0 0 5 ) ;食管癌MDM - 2与Bcl- 2染色阳性率间具有明显的相关关系 (χ2 =7 6 89,P <0 0 1)。结论 :食管癌常伴有HPV - 16感染 ,食管癌的发生与Bcl- 2和MDM -
Objective: Immunohistochemical studies of Bcl-2, MDM-2, and HPV-16 were carried out in the carcinoma of esophagus and its pre-cancerous lesions in order to investigate the cause and the mechanism responsible for the genesis of esophageal cancer. Methods: The comparative observation of Bcl-2, MDM-2 and HPV in the esophageal carcinoma and in its pre-cancerous lesions were conducted by means of immunohistochemical technique. Results: The positive staining of Bcl-2 was found in 1 case of normal esophageal epithelium (1/9,11.11%),and MDM-2 and HPV-16 showed totally negative. The positive staining of Bcl-2,MDM-2 and HPV-16 in the carcinoma was 84.61% (33/39),64.10%(25/39) and 33.33%(13/39)respectively. The positive rates of Bcl-2,MDM-2 and HPV-16 in the anaplasia were 22.58% (7/31), 35.48%(11/31) and 9.68% (3/31) respectively. There was a significant staining difference of Bcl-2,MDM-2 and HPV-16 among the three groups. Significant difference in infection rate of HPV-16 was also found between the esophageal (33.33%) carcinoma and atypical hyperplasia (9.68%). A close interrelation between Bcl-2 and MDM-2 staining was found in esophageal cancer. Conclusion: HPV-16 might be involved in the genesis of esophageal-cancer and abnormal high expression of Bcl-2 and MDM-2 genes is closely related to the pathogenesis of esophageal cancer and leads to runaway cell division.
出处
《宁夏医学院学报》
2004年第2期85-87,F004,共4页
Journal of Ningxia Medical College
