摘要
目的:观察心脏骤停患者心肺复苏后外周血中促炎/抗炎细胞因子、内皮素(ET-1)含量变化及其与预后的关系。方法:56例心脏骤停后心肺复苏患者依据复苏效果分为三组,A组:20例心肺复苏30 min无效死亡;B组:20例,复苏成功,自主循环建立 ≥ 2 h,但最终死亡;C组:16例,复苏成功,存活出院。选择20例健康门诊体检者作为对照D组。于心肺复苏即刻留取静脉血,用ELISA法检测外周血中白细胞介素(IL)-1β、TNF-α、IL-4和IL-10含量变化;采用放射免疫法检测ET-1含量。结果:与D组比较,A、B和C组外周血中IL-1β、TNF-α、IL-4、IL-10和ET-1含量明显增高(p <0.05);与C组比较,A和B组IL-1β、TNF-α、IL-4、IL-10和ET-1含量明显增高(p <0.05);与B组比较,A组IL-1β、TNF-α、IL-4和IL-10含量无明显不同,ET-1含量明显增高(p <0.05)。结论:促炎/抗炎细胞因子(IL-1β,TNF-α,IL-4,IL-10)异常释放与ET-1参与心肺复苏后缺血再灌注损伤的发生发展,且与心肺复苏预后密切相关。
Objective: To investigate the changes of ET-1 in the peripheral blood of patients with cardiopulmonary resuscitation and its relationship with prognosis. Methods: 56 patients with cardiopulmonary resuscitation after cardiac arrest were divided into group A (20 cases, cardiopulmonary resuscitation lasting for 30 min, then died), group B (20 cases, independent circulation was more than or equal to 2 h, but eventually died), and group C (16 cases, survival). Twenty healthy subjects were used as control (group D). Interleukin (IL)-1β, TNF-α, IL-4 and IL-10 content was determined by ELISA. ET-1 content was determined by radioimmunoassay. Results: Compared with group D, IL-1β, TNF-α, IL-4, IL-10 and ET-1 contents were significantly higher in peripheral blood of group A, B and C (p < 0.05). Compared with group C, IL-1β, TNF-α, IL-4, IL-10 and ET-1 contents were significantly increased in group A and B (p < 0.05). IL-1β, TNF-α, IL-4 and IL-10 content was not significantly different between group A and B, but the content of ET-1 was increased significantly in group B than group A (p < 0.05). Conclusion: The abnormal release of pro/ anti-inflammatory cytokine (IL-1β, TNF-α, IL-4, IL-10) and ET-1 is involved in the development of ischemia reperfusion injury after cardiopulmonary resuscitation, and is closely related with the prognosis of cardiopulmonary resuscitation.
出处
《亚洲急诊医学病例研究》
2017年第1期10-13,共4页
Asian Case Reports in Emergency Medicine
