摘要
Modulating healing factors could avoid or minimize some possible pathological processes in collagen deposition. The present study was aimed to evaluated the role of active biomolecules such as PDGF-BB and PRP loaded or not into polymeric biomaterial to seek potential mediators in types I and III collagen deposition and epithelization. The healing phases were investigated by using an in vivo full-thickness wound rat model. At zero, 3<sup>rd</sup>, 7<sup>th</sup> and 14<sup>th</sup> days after the experimental model, the size of the wound areas was photographed. The nanofibrous materials were biocompatible and did not cause any local adverse reaction and/or inflammation. On day 14 the wounds had healed almost 100% with better signs of healing, however there was no obvious difference in the wound contraction rates. At the end of 14 days, samples from the center of the lesion were collected when histological features and immunopositivity for collagen I and III expressions were assessed. There was no significant difference in the epithelization among the groups. Wounds treated with PRP and with PA-6/SOMA plus PDGF-BB had significantly lower amounts of type III collagen. The amounts of type I collagen did not have a statistically different deposition among the experimental groups. The association of PDGF-BB with PA-6/SOMA emerges as an alternative for topical application to unfavorable anatomical sites, suggesting that these associations may have a positive modulation on the process of accelerated healing remodeling.
Modulating healing factors could avoid or minimize some possible pathological processes in collagen deposition. The present study was aimed to evaluated the role of active biomolecules such as PDGF-BB and PRP loaded or not into polymeric biomaterial to seek potential mediators in types I and III collagen deposition and epithelization. The healing phases were investigated by using an in vivo full-thickness wound rat model. At zero, 3<sup>rd</sup>, 7<sup>th</sup> and 14<sup>th</sup> days after the experimental model, the size of the wound areas was photographed. The nanofibrous materials were biocompatible and did not cause any local adverse reaction and/or inflammation. On day 14 the wounds had healed almost 100% with better signs of healing, however there was no obvious difference in the wound contraction rates. At the end of 14 days, samples from the center of the lesion were collected when histological features and immunopositivity for collagen I and III expressions were assessed. There was no significant difference in the epithelization among the groups. Wounds treated with PRP and with PA-6/SOMA plus PDGF-BB had significantly lower amounts of type III collagen. The amounts of type I collagen did not have a statistically different deposition among the experimental groups. The association of PDGF-BB with PA-6/SOMA emerges as an alternative for topical application to unfavorable anatomical sites, suggesting that these associations may have a positive modulation on the process of accelerated healing remodeling.
作者
Anderson Ricardo Ingracio
Natália Fontana Nicoletti
Fernanda Trindade Gonzalez Dias
Manuela Peletti-Figueiró
Otávio Bianchi
Asdrubal Falavigna
Anderson Ricardo Ingracio;Natália Fontana Nicoletti;Fernanda Trindade Gonzalez Dias;Manuela Peletti-Figueiró;Otávio Bianchi;Asdrubal Falavigna(Health Sciences Graduate Program, Universidade de Caxias do Sul (UCS), Caxias do Sul, RS, Brazil;Cell Therapy Laboratory (LATEC), Universidade de Caxias do Sul (UCS), Caxias do Sul, RS, Brazil;Materials Science Graduate Program (PGMAT), Universidade de Caxias do Sul (UCS), Caxias do Sul, RS, Brazil)
