Bacterioplankton play key roles in the biogeochemical cycle and in organic contaminant degradation. The species richness and abundance of bacterial subgroups are generally distinct from each other, and this is attribu...Bacterioplankton play key roles in the biogeochemical cycle and in organic contaminant degradation. The species richness and abundance of bacterial subgroups are generally distinct from each other, and this is attributed to their different functions in aquatic ecosystems. The spatiotemporal variations of eight phylogenetic subgroups （Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, Planctomycetes, alpha-, beta-, and gamma-Proteobacteria） derived from Donghu Lake were investigated using PCR-DGGE fingerprinting, to explore their responses to environmental factors. Results indicate that Actinobacteria and beta-Proteobacteria were the two largest bacterial subgroups detected. These two groups and Bacteroidetes showed clear seasonal patterns in composition of the operational taxonomic unit. Results also suggest that the bacterioplankton subgroups in Donghu Lake were significantly correlated with different environmental factors. In brief, the total nitrogen was one of the major factors regulating all the bacterioplankton except for Actinobacteria. However, total phosphorus, another important eutrophication factor, contributed to the two largest bacterial groups （Actinobacteria and beta-Proteobacteria）, as well as to the Cyanobacteria and Firmicutes. Therefore, the responses of bacterioplankton subgroups to environmental factors were different, and this should be attributed to the differences in the fimctions of different groups.展开更多
基金Supported by the National Natural Science Foundation of China(No.31071896)the National Basic Research Program of China(973 Program)(No.2008CB418105)+2 种基金the Knowledge Innovation Program of Chinese Academy of Sciences(No.Y15E04)the Youth Innovation Promotion Association,Chinese Academy of Sciences(No.Y22Z07)the Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture(No.201007)
文摘Bacterioplankton play key roles in the biogeochemical cycle and in organic contaminant degradation. The species richness and abundance of bacterial subgroups are generally distinct from each other, and this is attributed to their different functions in aquatic ecosystems. The spatiotemporal variations of eight phylogenetic subgroups （Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, Planctomycetes, alpha-, beta-, and gamma-Proteobacteria） derived from Donghu Lake were investigated using PCR-DGGE fingerprinting, to explore their responses to environmental factors. Results indicate that Actinobacteria and beta-Proteobacteria were the two largest bacterial subgroups detected. These two groups and Bacteroidetes showed clear seasonal patterns in composition of the operational taxonomic unit. Results also suggest that the bacterioplankton subgroups in Donghu Lake were significantly correlated with different environmental factors. In brief, the total nitrogen was one of the major factors regulating all the bacterioplankton except for Actinobacteria. However, total phosphorus, another important eutrophication factor, contributed to the two largest bacterial groups （Actinobacteria and beta-Proteobacteria）, as well as to the Cyanobacteria and Firmicutes. Therefore, the responses of bacterioplankton subgroups to environmental factors were different, and this should be attributed to the differences in the fimctions of different groups.
