目的探讨千层纸素A(oroxylin A,OA)对鸡二型胶原诱导的关节炎(chicken type II collagen-induced arthritis,CIA)小鼠的治疗作用并观察免疫细胞在其中的变化。方法建立CIA小鼠模型,从第28天起,连续10 d腹腔注射40 mg/kg OA进行治疗,给...目的探讨千层纸素A(oroxylin A,OA)对鸡二型胶原诱导的关节炎(chicken type II collagen-induced arthritis,CIA)小鼠的治疗作用并观察免疫细胞在其中的变化。方法建立CIA小鼠模型,从第28天起,连续10 d腹腔注射40 mg/kg OA进行治疗,给药期间分3次处死小鼠,在每个时间节点对关节进行临床评分;HE染色观察小鼠踝关节病理情况;流式细胞仪检测小鼠脾脏以及腹股沟淋巴结中Th17、Treg以及巨噬细胞在治疗过程中的变化;ELISA检测小鼠血清中IL-1β、IL-18和IL-6的表达水平;qRT-PCR检测小鼠脾脏中IL-1β、IL-18、IL-6和TNF-α的表达水平。结果OA给药后第34天起,CIA小鼠关节肿胀程度明显缓解,病理评分降低,炎性细胞浸润减少;流式细胞术结果显示,OA组的CIA小鼠脾脏和腹股沟淋巴结中Th17细胞、巨噬细胞比例下降,而Treg细胞比例增加;ELISA和qRT-PCR结果表明,OA可以抑制CIA小鼠体内的炎症水平。结论OA可以通过调节免疫细胞的比例抑制CIA小鼠体内的炎症水平,进而缓解CIA小鼠的症状。展开更多
Radioimmunoassay,enzyme linked immunospot assay and enzyme immunoassay were used for the determinations of plasma steroid hormone's level,antibody producing cell's counting and IgM level in this study.The de...Radioimmunoassay,enzyme linked immunospot assay and enzyme immunoassay were used for the determinations of plasma steroid hormone's level,antibody producing cell's counting and IgM level in this study.The decreased number of antibody producing cells and low IgM levels were observed in sexual immature rainbow trout during the spawning season.These fish were reared under almost constant water temperature and natural photoperiod.Moreover,low IgM level was also observed in immature rainbow trout,which were reared under short photoperiod,and IgM level was not changed by treatment of testosterone.The results suggest that photoperoid may cause the changes in immune competence.It is possible that circadian rhythm accompanied with photoperiod may influence physiological function of fish,so that immune competence is changed.展开更多
The relationships between 17α,20β dihydroxy 4 pregnene 3 one (17α,20β DP) and plasma IgM and total protein levels were investigated in rainbow trout, Oncorhynchus mykiss .IgM and total protein levels decre...The relationships between 17α,20β dihydroxy 4 pregnene 3 one (17α,20β DP) and plasma IgM and total protein levels were investigated in rainbow trout, Oncorhynchus mykiss .IgM and total protein levels decreased in both sexes of mature rainbow trout when 17α,20β DP levels increased during the spawning season,while the elevated 17α,20β DP suppressed IgM levels and reversibly enhanced total protein concentrations in immature trout.This represents the first report of the effect of 17α,20β DP on fish immunity.These data suggest that the increase of 17α,20β DP in spawning season may be related to infectious skin diseases.展开更多
文摘目的探讨千层纸素A(oroxylin A,OA)对鸡二型胶原诱导的关节炎(chicken type II collagen-induced arthritis,CIA)小鼠的治疗作用并观察免疫细胞在其中的变化。方法建立CIA小鼠模型,从第28天起,连续10 d腹腔注射40 mg/kg OA进行治疗,给药期间分3次处死小鼠,在每个时间节点对关节进行临床评分;HE染色观察小鼠踝关节病理情况;流式细胞仪检测小鼠脾脏以及腹股沟淋巴结中Th17、Treg以及巨噬细胞在治疗过程中的变化;ELISA检测小鼠血清中IL-1β、IL-18和IL-6的表达水平;qRT-PCR检测小鼠脾脏中IL-1β、IL-18、IL-6和TNF-α的表达水平。结果OA给药后第34天起,CIA小鼠关节肿胀程度明显缓解,病理评分降低,炎性细胞浸润减少;流式细胞术结果显示,OA组的CIA小鼠脾脏和腹股沟淋巴结中Th17细胞、巨噬细胞比例下降,而Treg细胞比例增加;ELISA和qRT-PCR结果表明,OA可以抑制CIA小鼠体内的炎症水平。结论OA可以通过调节免疫细胞的比例抑制CIA小鼠体内的炎症水平,进而缓解CIA小鼠的症状。
文摘Radioimmunoassay,enzyme linked immunospot assay and enzyme immunoassay were used for the determinations of plasma steroid hormone's level,antibody producing cell's counting and IgM level in this study.The decreased number of antibody producing cells and low IgM levels were observed in sexual immature rainbow trout during the spawning season.These fish were reared under almost constant water temperature and natural photoperiod.Moreover,low IgM level was also observed in immature rainbow trout,which were reared under short photoperiod,and IgM level was not changed by treatment of testosterone.The results suggest that photoperoid may cause the changes in immune competence.It is possible that circadian rhythm accompanied with photoperiod may influence physiological function of fish,so that immune competence is changed.
文摘The relationships between 17α,20β dihydroxy 4 pregnene 3 one (17α,20β DP) and plasma IgM and total protein levels were investigated in rainbow trout, Oncorhynchus mykiss .IgM and total protein levels decreased in both sexes of mature rainbow trout when 17α,20β DP levels increased during the spawning season,while the elevated 17α,20β DP suppressed IgM levels and reversibly enhanced total protein concentrations in immature trout.This represents the first report of the effect of 17α,20β DP on fish immunity.These data suggest that the increase of 17α,20β DP in spawning season may be related to infectious skin diseases.
