在丝网印刷碳电极上,采用层层自组装法制备普鲁士蓝薄膜,同时基于戊二醛交联法在薄膜上固定葡萄糖氧化酶,从而构建一种高灵敏度、低成本的葡萄糖生物传感器。考察组装温度和层数对薄膜形貌的影响。在最佳组装条件35℃和40层下,获得了均...在丝网印刷碳电极上,采用层层自组装法制备普鲁士蓝薄膜,同时基于戊二醛交联法在薄膜上固定葡萄糖氧化酶,从而构建一种高灵敏度、低成本的葡萄糖生物传感器。考察组装温度和层数对薄膜形貌的影响。在最佳组装条件35℃和40层下,获得了均匀、连续分布的具有纳米立方颗粒结构的普鲁士蓝薄膜。普鲁士蓝立方结构有利于薄膜催化活性的提高,而戊二醛交联法可有效用于酶的固定并保持酶的活性,从而提高传感器的灵敏度和稳定性。在-0.05 V工作电位下,制备的传感器具有超高的灵敏度(111.834 m A/(mol·L-1·cm2)),宽的线性范围(0~1.2 mmol/L),低的检测极限(1μmol/L),同时具有优良的重复性、稳定性和抗干扰能力。本研究中,普鲁士蓝薄膜的制备及酶的固定简单易行,同时结合丝网印刷技术,可实现葡萄糖生物传感器的批量化制备,具有显著的应用前景。展开更多
Photocatalytic degradation is one of the most promising remediation technologies in terms of advanced oxidation processes(AOPs) for water treatment. In this study, novel graphitic carbon nitride/titanium dioxide(gC3N4...Photocatalytic degradation is one of the most promising remediation technologies in terms of advanced oxidation processes(AOPs) for water treatment. In this study, novel graphitic carbon nitride/titanium dioxide(gC3N4/Ti O2) composites were synthesized by a facile sonication method. The physicochemical properties of the photocatalyst with different mass ratios of g-C3N4 to Ti O2 were investigated by X-ray diffraction(XRD), scanning electron microscope(SEM), transmission electron microscopy(TEM), N2 sorption, Fourier transform infrared spectroscopy(FT-IR), X-ray photoelectron spectroscopy(XPS), and UV–vis DRS. The photocatalytic performances were evaluated by degradation of methylene blue. It was found that g-C3N4/Ti O2 with a mass ratio of 1.5:1 exhibited the best degradation performance. Under UV, the degradation rate of g-C3N4/Ti O2 was 6.92 and 2.65 times higher than g-C3N4 and Ti O2, respectively. While under visible light, the enhancement factors became 9.27(to g-C3N4) and 7.03(to Ti O2). The improved photocatalytic activity was ascribed to the interfacial charge transfer between g-C3N4 and Ti O2. This work suggests that hybridization can produce promising solar materials for environmental remediation.展开更多
文摘在丝网印刷碳电极上,采用层层自组装法制备普鲁士蓝薄膜,同时基于戊二醛交联法在薄膜上固定葡萄糖氧化酶,从而构建一种高灵敏度、低成本的葡萄糖生物传感器。考察组装温度和层数对薄膜形貌的影响。在最佳组装条件35℃和40层下,获得了均匀、连续分布的具有纳米立方颗粒结构的普鲁士蓝薄膜。普鲁士蓝立方结构有利于薄膜催化活性的提高,而戊二醛交联法可有效用于酶的固定并保持酶的活性,从而提高传感器的灵敏度和稳定性。在-0.05 V工作电位下,制备的传感器具有超高的灵敏度(111.834 m A/(mol·L-1·cm2)),宽的线性范围(0~1.2 mmol/L),低的检测极限(1μmol/L),同时具有优良的重复性、稳定性和抗干扰能力。本研究中,普鲁士蓝薄膜的制备及酶的固定简单易行,同时结合丝网印刷技术,可实现葡萄糖生物传感器的批量化制备,具有显著的应用前景。
基金Supported by the Innovative Research Team Program by the Ministry of Education of China(IRT13070)the Nature Science Foundation of Jiangsu Province(BK2012423,BK20130925)the Opening Project of State Key Laboratory of Materials-Oriented Chemical Engineering of China(KL13-02)
文摘Photocatalytic degradation is one of the most promising remediation technologies in terms of advanced oxidation processes(AOPs) for water treatment. In this study, novel graphitic carbon nitride/titanium dioxide(gC3N4/Ti O2) composites were synthesized by a facile sonication method. The physicochemical properties of the photocatalyst with different mass ratios of g-C3N4 to Ti O2 were investigated by X-ray diffraction(XRD), scanning electron microscope(SEM), transmission electron microscopy(TEM), N2 sorption, Fourier transform infrared spectroscopy(FT-IR), X-ray photoelectron spectroscopy(XPS), and UV–vis DRS. The photocatalytic performances were evaluated by degradation of methylene blue. It was found that g-C3N4/Ti O2 with a mass ratio of 1.5:1 exhibited the best degradation performance. Under UV, the degradation rate of g-C3N4/Ti O2 was 6.92 and 2.65 times higher than g-C3N4 and Ti O2, respectively. While under visible light, the enhancement factors became 9.27(to g-C3N4) and 7.03(to Ti O2). The improved photocatalytic activity was ascribed to the interfacial charge transfer between g-C3N4 and Ti O2. This work suggests that hybridization can produce promising solar materials for environmental remediation.
