The proliferation of vascular smooth muscle cells(VSMCs)contributes to the pathogenesis of atherosclerosis and restenosis after angioplasty and vein graft.In this study,MTT colormetry was used to test the effective sc...The proliferation of vascular smooth muscle cells(VSMCs)contributes to the pathogenesis of atherosclerosis and restenosis after angioplasty and vein graft.In this study,MTT colormetry was used to test the effective scope of emodin to inhibit VSMCs proliferation.Flow cytometry and confocal image were adopted to investigate its inhibitive mechanism.The results show that emodin could inhibit the growth and proliferation of VSMCs and the inhibition rate of emodin on VSMCs is 24.6%-94.58%,which is time-and concentration-dependent.Emodin could reduce S phase entry,increase the apoptosis of VSMCs,and reduce the intensity of[Ca^(2+)]_(i)in hPDGF B/B stimulated VSMCs.This research provides theoretical basis for medical application of emodin.It is concluded that emodin could inhibit the growth and proliferation of VSMCs effectively.Decreasing the DNA synthesis,increasing the cell apoptosis and reducing the intensity of[Ca^(2+)]_(i)in hPDGF B/B stimulated VSMCs may be the inhibitive mechanism of emodin against VSMCs proliferation.展开更多
Phospholipids are believed to be important biomaterials. However, limited information is available on their cytocompatibilities. The objective of this study is to evaluate the effects of different phospholipids on the...Phospholipids are believed to be important biomaterials. However, limited information is available on their cytocompatibilities. The objective of this study is to evaluate the effects of different phospholipids on the proliferation of hepatic Bel\|7402 cells by comparing the adhesion, viability and proliferation of Bel\|7402 cells cultured on different phospholipid surfaces. The cell adhesion, determined by counting the number of adhered cells to the surface, indicated that the cell adhesion was enhanced on charged phospolipid membranes. The cell viability evaluated by MTT\[3\|(4,5\|dimethylthiazole\|2\|yl)\|2,5\|diphenyl tetrazolium\|bromide\] showed that cells cultured on charged phospholipids have greater viability than those cultured on the control, while cells cultured on neutral phospholipids showed lower viability. The cell cycle analysis using flow cytometry demonstrated that S phase entry increased on charged phospholipids, while S phase entry decreased on neutral phospholipids. The results suggested that charged phospholipids, especially positively charged phospholipids, show better cytocompatibilities than neutral phospholipids to hepatic Bel\|7402 cell.展开更多
文摘The proliferation of vascular smooth muscle cells(VSMCs)contributes to the pathogenesis of atherosclerosis and restenosis after angioplasty and vein graft.In this study,MTT colormetry was used to test the effective scope of emodin to inhibit VSMCs proliferation.Flow cytometry and confocal image were adopted to investigate its inhibitive mechanism.The results show that emodin could inhibit the growth and proliferation of VSMCs and the inhibition rate of emodin on VSMCs is 24.6%-94.58%,which is time-and concentration-dependent.Emodin could reduce S phase entry,increase the apoptosis of VSMCs,and reduce the intensity of[Ca^(2+)]_(i)in hPDGF B/B stimulated VSMCs.This research provides theoretical basis for medical application of emodin.It is concluded that emodin could inhibit the growth and proliferation of VSMCs effectively.Decreasing the DNA synthesis,increasing the cell apoptosis and reducing the intensity of[Ca^(2+)]_(i)in hPDGF B/B stimulated VSMCs may be the inhibitive mechanism of emodin against VSMCs proliferation.
基金theNationalNaturalScienceFoundationof China! (No .5 94932 0 8)
文摘Phospholipids are believed to be important biomaterials. However, limited information is available on their cytocompatibilities. The objective of this study is to evaluate the effects of different phospholipids on the proliferation of hepatic Bel\|7402 cells by comparing the adhesion, viability and proliferation of Bel\|7402 cells cultured on different phospholipid surfaces. The cell adhesion, determined by counting the number of adhered cells to the surface, indicated that the cell adhesion was enhanced on charged phospolipid membranes. The cell viability evaluated by MTT\[3\|(4,5\|dimethylthiazole\|2\|yl)\|2,5\|diphenyl tetrazolium\|bromide\] showed that cells cultured on charged phospholipids have greater viability than those cultured on the control, while cells cultured on neutral phospholipids showed lower viability. The cell cycle analysis using flow cytometry demonstrated that S phase entry increased on charged phospholipids, while S phase entry decreased on neutral phospholipids. The results suggested that charged phospholipids, especially positively charged phospholipids, show better cytocompatibilities than neutral phospholipids to hepatic Bel\|7402 cell.
