系统性红斑狼疮(SLE)是一种影响多个器官的全身性自身免疫疾病,其特点是免疫细胞、免疫因子和免疫途径的复杂相互作用导致各种临床表现。抗中性粒细胞胞浆抗体(ANCA)相关性血管炎(AAV)是一组以中小血管坏死性炎症和外周血中出现ANCA为...系统性红斑狼疮(SLE)是一种影响多个器官的全身性自身免疫疾病,其特点是免疫细胞、免疫因子和免疫途径的复杂相互作用导致各种临床表现。抗中性粒细胞胞浆抗体(ANCA)相关性血管炎(AAV)是一组以中小血管坏死性炎症和外周血中出现ANCA为特征的自身免疫综合征。SLE/AAV重叠综合征(overlap syndrome, OS)一种混合表现的炎症性疾病,具有SLE和AAV的临床特征。在极少数情况下,SLE和AAV同时发病表现出更严重的临床症状。缺血性结肠炎(IC)是一组存在血管闭塞性或非闭塞性疾病的临床综合征,以结肠供血不足为特征。本文报告了1例SLE/AAV OS伴随缺血性结肠炎患者。经过血浆置换、足量激素冲击、免疫抑制剂环磷酰胺和硫酸羟氯喹、抑酸药、生长激素抑制类药物等治疗后,患者病情缓解。Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that affects multiple organs and is characterized by a complex interplay of immune cells, immune factors, and immune pathways leading to a variety of clinical manifestations. Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a group of autoimmune syndromes characterized by necrotizing inflammation of small and medium-sized blood vessels and the presence of ANCA in the peripheral blood. Overlap syndrome (OS) is a mixed manifestation of inflammatory disease with clinical features of SLE and AAV. In rare cases, the concurrent onset of SLE and AAV results in SLE/AAV OS, which exhibits more severe clinical symptoms. Ischemic colitis (IC) is a group of clinical syndromes in the presence of vaso-occlusive or non-occlusive disorders characterized by inadequate blood supply to the colon. In this article, we report a patient with SLE/AAV OS combined with ischemic colitis. After treatment with adequate hormonal shocks, the immunosuppressants cyclophosphamide and hydroxychloroquine sulfate, plasma exchange, acid-suppressing drugs, and anti-growth hormone analogs, the patient's condition resolved.展开更多
以往的研究主要集中在肿瘤细胞在免疫逃逸中的变化,而对肿瘤微环境(tumor microenvironment, TME)对免疫逃逸的影响知之甚少。肿瘤相关成纤维细胞(tumor-associated fibroblasts, TAFs)是TME的重要组成部分,具有特殊的生理生化特性,但...以往的研究主要集中在肿瘤细胞在免疫逃逸中的变化,而对肿瘤微环境(tumor microenvironment, TME)对免疫逃逸的影响知之甚少。肿瘤相关成纤维细胞(tumor-associated fibroblasts, TAFs)是TME的重要组成部分,具有特殊的生理生化特性,但其具体机制尚不清楚。为了研究TAF对胃癌细胞PD-L1表达的影响,通过transwell将胃癌细胞株MNK45、SGC7901与TAFs非接触共培养1、3、7 d。采用qRT-PCR和流式细胞仪检测PD-L1 mRNA和蛋白表达。然后选择95例胃癌组织,通过免疫组化检测PD-L1和TAFs的表达。结果显示,实验组PD-L1 mRNA和蛋白表达量均显著高于对照组。胃癌中PD-L1的表达与大量淋巴细胞浸润、弥漫性/混合性组织学和瘤内TAFs有关。综上所述,TAFs通过提高PD-L1的表达促进胃癌细胞株的生长。Previous studies have focused on the changes of tumour cells in immune escape, and less is known about the effect of the tumour microenvironment (TME) on immune escape. Tumour-associated fibroblasts (TAFs) cells are an important part of the TME and have special physiological and biochemical characteristics, but the specific mechanism has not been clarified. In order to investigate the effect of TAFs on the expression of PD-L1 in gastric cancer cells, gastric cancer cell lines MNK45, SGC7901 were non-contact coculturing with TAFs 1, 3 and 7 d via transwell. PD-L1 mRNA and protein expression were detected using qRT-PCR and FCM. Then, 95 cases of gastric cancer tissues were selected and PD-L1 and TAFs expressions were determined by immunohistochemical examination. The results showed that the mRNA and protein expression of PD-L1 in the experiment group were significantly higher than that in the control group. PD-L1 expression was associated with massive lymphocyte infiltration, diffuse/mixed histology and intratumoral TAFs in gastric cancers. In conclusion, TAFs promoted the growth of gastric cancer cell lines by increasing the PD-L1 expression.展开更多
为探究大豆分离蛋白(Soy protein isolate,SPI)结构变化对茄替胶-改性大豆分离蛋白共价复合物乳化性能的影响。文章以SPI为原料,采用超声波处理得到USPI、超声结合热处理得到UHSPI,与茄替胶(Gum ghatti,GG)在温度80℃、相对湿度79%条件...为探究大豆分离蛋白(Soy protein isolate,SPI)结构变化对茄替胶-改性大豆分离蛋白共价复合物乳化性能的影响。文章以SPI为原料,采用超声波处理得到USPI、超声结合热处理得到UHSPI,与茄替胶(Gum ghatti,GG)在温度80℃、相对湿度79%条件下进行美拉德反应,制备获得GG-改性SPI共价复合物。傅里叶变换红外光谱和圆二色谱结果表明,超声和加热影响SPI二级结构,α-螺旋相对含量减少,β-折叠相对含量增加,证实改性SPI和GG发生共价结合。pH为7时,采用超声结合热处理工艺,SPI溶解度由22.31%提高至47.68%,表面疏水性从104.13提高至162.19。pH为4.5时,与SPI相较,美拉德反应制备得到的GG-UHSPI-MR复合物溶解度提高5倍,表现出在酸性条件下应用的可能性。褐变程度结果表明,溶解度高的UHSPI样品更易和GG间形成共价键。水包油乳液特征分析结果表明,储存14 d后,GG-UHSPI-MR乳液粒径(D 4,3)最小为0.672μm,乳液稳定性较好。综上表明,GG和UHSPI共价结合后乳化性能提高,有望作为一种新型乳化剂在乳制品领域应用。展开更多
文摘系统性红斑狼疮(SLE)是一种影响多个器官的全身性自身免疫疾病,其特点是免疫细胞、免疫因子和免疫途径的复杂相互作用导致各种临床表现。抗中性粒细胞胞浆抗体(ANCA)相关性血管炎(AAV)是一组以中小血管坏死性炎症和外周血中出现ANCA为特征的自身免疫综合征。SLE/AAV重叠综合征(overlap syndrome, OS)一种混合表现的炎症性疾病,具有SLE和AAV的临床特征。在极少数情况下,SLE和AAV同时发病表现出更严重的临床症状。缺血性结肠炎(IC)是一组存在血管闭塞性或非闭塞性疾病的临床综合征,以结肠供血不足为特征。本文报告了1例SLE/AAV OS伴随缺血性结肠炎患者。经过血浆置换、足量激素冲击、免疫抑制剂环磷酰胺和硫酸羟氯喹、抑酸药、生长激素抑制类药物等治疗后,患者病情缓解。Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that affects multiple organs and is characterized by a complex interplay of immune cells, immune factors, and immune pathways leading to a variety of clinical manifestations. Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a group of autoimmune syndromes characterized by necrotizing inflammation of small and medium-sized blood vessels and the presence of ANCA in the peripheral blood. Overlap syndrome (OS) is a mixed manifestation of inflammatory disease with clinical features of SLE and AAV. In rare cases, the concurrent onset of SLE and AAV results in SLE/AAV OS, which exhibits more severe clinical symptoms. Ischemic colitis (IC) is a group of clinical syndromes in the presence of vaso-occlusive or non-occlusive disorders characterized by inadequate blood supply to the colon. In this article, we report a patient with SLE/AAV OS combined with ischemic colitis. After treatment with adequate hormonal shocks, the immunosuppressants cyclophosphamide and hydroxychloroquine sulfate, plasma exchange, acid-suppressing drugs, and anti-growth hormone analogs, the patient's condition resolved.
文摘以往的研究主要集中在肿瘤细胞在免疫逃逸中的变化,而对肿瘤微环境(tumor microenvironment, TME)对免疫逃逸的影响知之甚少。肿瘤相关成纤维细胞(tumor-associated fibroblasts, TAFs)是TME的重要组成部分,具有特殊的生理生化特性,但其具体机制尚不清楚。为了研究TAF对胃癌细胞PD-L1表达的影响,通过transwell将胃癌细胞株MNK45、SGC7901与TAFs非接触共培养1、3、7 d。采用qRT-PCR和流式细胞仪检测PD-L1 mRNA和蛋白表达。然后选择95例胃癌组织,通过免疫组化检测PD-L1和TAFs的表达。结果显示,实验组PD-L1 mRNA和蛋白表达量均显著高于对照组。胃癌中PD-L1的表达与大量淋巴细胞浸润、弥漫性/混合性组织学和瘤内TAFs有关。综上所述,TAFs通过提高PD-L1的表达促进胃癌细胞株的生长。Previous studies have focused on the changes of tumour cells in immune escape, and less is known about the effect of the tumour microenvironment (TME) on immune escape. Tumour-associated fibroblasts (TAFs) cells are an important part of the TME and have special physiological and biochemical characteristics, but the specific mechanism has not been clarified. In order to investigate the effect of TAFs on the expression of PD-L1 in gastric cancer cells, gastric cancer cell lines MNK45, SGC7901 were non-contact coculturing with TAFs 1, 3 and 7 d via transwell. PD-L1 mRNA and protein expression were detected using qRT-PCR and FCM. Then, 95 cases of gastric cancer tissues were selected and PD-L1 and TAFs expressions were determined by immunohistochemical examination. The results showed that the mRNA and protein expression of PD-L1 in the experiment group were significantly higher than that in the control group. PD-L1 expression was associated with massive lymphocyte infiltration, diffuse/mixed histology and intratumoral TAFs in gastric cancers. In conclusion, TAFs promoted the growth of gastric cancer cell lines by increasing the PD-L1 expression.
