DNA分子水平上的多态性检测技术是进行基因组研究的基础。自从1975年Crodzicker等人第一次利用RFLP(Restriction Fragment Length Polymorphism限制片段长度多态性)进行腺病毒血清型突变体基因组作图以来,人们便开始广泛利用DNA分子水...DNA分子水平上的多态性检测技术是进行基因组研究的基础。自从1975年Crodzicker等人第一次利用RFLP(Restriction Fragment Length Polymorphism限制片段长度多态性)进行腺病毒血清型突变体基因组作图以来,人们便开始广泛利用DNA分子水平上的多态性进行基因组遗传图谱构建。展开更多
The ITS-Is of 24 accessions belong to 10 species of subgenus Glycine, and 2 species of subgenus Soja of genus Glycine were amplified, cloned and sequenced. According to the homology of the sequences, the phy-logeny of...The ITS-Is of 24 accessions belong to 10 species of subgenus Glycine, and 2 species of subgenus Soja of genus Glycine were amplified, cloned and sequenced. According to the homology of the sequences, the phy-logeny of the 24 accessions were reconstructed. The reconstructed dendrogram showed that there were some divergent genomic types found in the previously classified species, such as G . tomentella, G. canescens and G. tabacina, and they might be some cryptic species by morphologic analysis.展开更多
The detection and exploitation of naturally occurring DNA sequencepolymorphisms represent one of the most significant developments in molecular biology.Restriction endonuclease digestion of total genomic DNA followed ...The detection and exploitation of naturally occurring DNA sequencepolymorphisms represent one of the most significant developments in molecular biology.Restriction endonuclease digestion of total genomic DNA followed by hybridizationwith a radioactively labelled probe reveals differently sized hybridizing fragments. Thisform of polymorphism termed RFLP has been used extensively for genetic studies.However, the technique is not so efficient in detecting polymorphisms of展开更多
The rapid development of molecular biology resulted in the wide application of popula-tion genetics theory to plant pathology.It is the development of efficient molecular DNAmarker systems that gives a great insight i...The rapid development of molecular biology resulted in the wide application of popula-tion genetics theory to plant pathology.It is the development of efficient molecular DNAmarker systems that gives a great insight into the population genetics of a number offungal plant pathogens such as rice blast fungus Magnaporthe grisea,late blight fungusPhytophthora infestans,barley powdery mildew fungus Erysiphe graminis f.sp.hordeiand wheat blotch fungus Septoria tritici.The knowledge on population biology of plantpathogens is essential for screening and identification of disease resistance genes,for devel-展开更多
Soybeans originated in China; therefore, their genetic germplasms, especially of wildsoybeans, are abundant in China. In recent years. more than 5000 accessions of wildsoybeans which account for about 90％ of the worl...Soybeans originated in China; therefore, their genetic germplasms, especially of wildsoybeans, are abundant in China. In recent years. more than 5000 accessions of wildsoybeans which account for about 90％ of the world collections of wild soybeans, have beencollected. These materials not only provide new sources of genes, but also can be used asthe basic materials in the biological study of soybeans. A previous study has shown thatthere are numerous ecological types, and the genetic diversity is rich in Chinese wildsoybean germplasms. A comparison study between wild and cultivated soybeans not onlyprovides basic information for the usage of wild soybeans in soybean breeding, but also fora better understanding of soybean origination and evolution.展开更多
文摘DNA分子水平上的多态性检测技术是进行基因组研究的基础。自从1975年Crodzicker等人第一次利用RFLP(Restriction Fragment Length Polymorphism限制片段长度多态性)进行腺病毒血清型突变体基因组作图以来,人们便开始广泛利用DNA分子水平上的多态性进行基因组遗传图谱构建。
基金Project supported by the Chinese Academy of Sciences.
文摘The ITS-Is of 24 accessions belong to 10 species of subgenus Glycine, and 2 species of subgenus Soja of genus Glycine were amplified, cloned and sequenced. According to the homology of the sequences, the phy-logeny of the 24 accessions were reconstructed. The reconstructed dendrogram showed that there were some divergent genomic types found in the previously classified species, such as G . tomentella, G. canescens and G. tabacina, and they might be some cryptic species by morphologic analysis.
文摘The detection and exploitation of naturally occurring DNA sequencepolymorphisms represent one of the most significant developments in molecular biology.Restriction endonuclease digestion of total genomic DNA followed by hybridizationwith a radioactively labelled probe reveals differently sized hybridizing fragments. Thisform of polymorphism termed RFLP has been used extensively for genetic studies.However, the technique is not so efficient in detecting polymorphisms of
基金Project supported by the Climbing Program of Chinathe Plant Biotechnology Laboratory, Chinese Academy of Sciences.
文摘The rapid development of molecular biology resulted in the wide application of popula-tion genetics theory to plant pathology.It is the development of efficient molecular DNAmarker systems that gives a great insight into the population genetics of a number offungal plant pathogens such as rice blast fungus Magnaporthe grisea,late blight fungusPhytophthora infestans,barley powdery mildew fungus Erysiphe graminis f.sp.hordeiand wheat blotch fungus Septoria tritici.The knowledge on population biology of plantpathogens is essential for screening and identification of disease resistance genes,for devel-
基金Project supported partly by the National Natural Science Foundation of China.
文摘Soybeans originated in China; therefore, their genetic germplasms, especially of wildsoybeans, are abundant in China. In recent years. more than 5000 accessions of wildsoybeans which account for about 90％ of the world collections of wild soybeans, have beencollected. These materials not only provide new sources of genes, but also can be used asthe basic materials in the biological study of soybeans. A previous study has shown thatthere are numerous ecological types, and the genetic diversity is rich in Chinese wildsoybean germplasms. A comparison study between wild and cultivated soybeans not onlyprovides basic information for the usage of wild soybeans in soybean breeding, but also fora better understanding of soybean origination and evolution.
