潜标系统在海洋工程,海洋观测和海洋养殖中被广泛应用。本文构建了潜标系统的动力学模型,在多种海洋环境中针对于以潜标为模型的单点系泊系统,讨论了其投放过程中的动力学响应和系统受到的载荷。文中采用集中质量下多边形近似法讨论了...潜标系统在海洋工程,海洋观测和海洋养殖中被广泛应用。本文构建了潜标系统的动力学模型,在多种海洋环境中针对于以潜标为模型的单点系泊系统,讨论了其投放过程中的动力学响应和系统受到的载荷。文中采用集中质量下多边形近似法讨论了系泊系统的动力学特性并考虑了节点之间水动力作用和缆绳的弹性形变。在时域内搭建系泊系统动力学模型并对系统中各节点的运动、整体姿态变化和缆绳受力状况进行分析。在布放过程中,通过分析潜标系统在不同海洋条件下的定位数据以及各个系统部分所受到的水动力影响和标体布放时的姿态变化规律,利用了水动力分析软件OrcaFlex构建了一套简化模型。其涵盖了在各种海况和工作状态下的情况,旨在精确计算系统在布放阶段的动态响应。The buoy system is one of the most commonly used positioning methods in the fields of marine engineering, ocean observation, and marine aquaculture. In this paper, the dynamics modeling of the buoy system is carried out. The dynamic response and load of the single point mooring system based on the model of the buoy system during deployment process are studied in different marine environments. The polygon approximation method based on concentrated mass is used to study the dynamics performance of the mooring system. Considering the hydrodynamic force of each node and the elastic deformation of the cable, the dynamics model of the mooring system was carried out in the time domain, analyzing the motion of the nodes, the attitude of the system and the force of the cable in the mooring system. Considering the positioning information of the buoy system during the deployment process, as well as the hydrodynamic effects of each part of the system and the attitude changes during the deployment process of the buoy, a simplified hydrodynamic analysis model under different sea conditions and different working conditions is established by using the hydrodynamic analysis software OrcaFlex, and the dynamic response of the system during the deployment process is calculated.展开更多
根据樟叶越桔Vaccinium dunalianum叶芽转录组测序实验获得的糖基转移酶Vd UGT1基因部分c DNA序列设计引物,采用RACE-PCR技术克隆了全长1 620 bp c DNA序列的Vd UGT1基因,包括1 398 bp c DNA序列的完整开放阅读框,推测编码由465个氨基...根据樟叶越桔Vaccinium dunalianum叶芽转录组测序实验获得的糖基转移酶Vd UGT1基因部分c DNA序列设计引物,采用RACE-PCR技术克隆了全长1 620 bp c DNA序列的Vd UGT1基因,包括1 398 bp c DNA序列的完整开放阅读框,推测编码由465个氨基酸残基组成、相对分子质量为50.89 k D的糖基转移酶Vd UGT1。序列分析表明,Vd UGT1理论等电点为5.53,负电荷残基(Asp+Glu)总数为53个,正电荷残基(Arg+Lys)总数为41个,不稳定系数为48.38,属于不稳定蛋白;其二级结构的主要构件为α-螺旋和随机卷曲,无跨膜结构域,属于亲水性蛋白质。Vd UGT1位于C末端含有尿嘧啶核苷二磷酸糖基转移酶所特有UDPGT功能域,推测与尿嘧啶核苷二磷酸糖的结合有关。该研究为后期Vd UGT1的异源表达和功能研究奠定了基础。展开更多
文摘潜标系统在海洋工程,海洋观测和海洋养殖中被广泛应用。本文构建了潜标系统的动力学模型,在多种海洋环境中针对于以潜标为模型的单点系泊系统,讨论了其投放过程中的动力学响应和系统受到的载荷。文中采用集中质量下多边形近似法讨论了系泊系统的动力学特性并考虑了节点之间水动力作用和缆绳的弹性形变。在时域内搭建系泊系统动力学模型并对系统中各节点的运动、整体姿态变化和缆绳受力状况进行分析。在布放过程中,通过分析潜标系统在不同海洋条件下的定位数据以及各个系统部分所受到的水动力影响和标体布放时的姿态变化规律,利用了水动力分析软件OrcaFlex构建了一套简化模型。其涵盖了在各种海况和工作状态下的情况,旨在精确计算系统在布放阶段的动态响应。The buoy system is one of the most commonly used positioning methods in the fields of marine engineering, ocean observation, and marine aquaculture. In this paper, the dynamics modeling of the buoy system is carried out. The dynamic response and load of the single point mooring system based on the model of the buoy system during deployment process are studied in different marine environments. The polygon approximation method based on concentrated mass is used to study the dynamics performance of the mooring system. Considering the hydrodynamic force of each node and the elastic deformation of the cable, the dynamics model of the mooring system was carried out in the time domain, analyzing the motion of the nodes, the attitude of the system and the force of the cable in the mooring system. Considering the positioning information of the buoy system during the deployment process, as well as the hydrodynamic effects of each part of the system and the attitude changes during the deployment process of the buoy, a simplified hydrodynamic analysis model under different sea conditions and different working conditions is established by using the hydrodynamic analysis software OrcaFlex, and the dynamic response of the system during the deployment process is calculated.
文摘根据樟叶越桔Vaccinium dunalianum叶芽转录组测序实验获得的糖基转移酶Vd UGT1基因部分c DNA序列设计引物,采用RACE-PCR技术克隆了全长1 620 bp c DNA序列的Vd UGT1基因,包括1 398 bp c DNA序列的完整开放阅读框,推测编码由465个氨基酸残基组成、相对分子质量为50.89 k D的糖基转移酶Vd UGT1。序列分析表明,Vd UGT1理论等电点为5.53,负电荷残基(Asp+Glu)总数为53个,正电荷残基(Arg+Lys)总数为41个,不稳定系数为48.38,属于不稳定蛋白;其二级结构的主要构件为α-螺旋和随机卷曲,无跨膜结构域,属于亲水性蛋白质。Vd UGT1位于C末端含有尿嘧啶核苷二磷酸糖基转移酶所特有UDPGT功能域,推测与尿嘧啶核苷二磷酸糖的结合有关。该研究为后期Vd UGT1的异源表达和功能研究奠定了基础。
文摘作为最丰富的油体结合蛋白,油质蛋白在油体的发生到分解消失过程中起着重要的生物学功能。本实验应用生物信息学和分子生物学技术分离克隆了麻疯树Jatrophacurcas全长593 bp的油质蛋白JcOle14.3基因序列,无内含子结构。JcOle14.3基因转录的m RNA包含了完整的开放阅读框,推测编码由137个氨基酸残基组成、相对分子质量为14.3 k D的稳定、两性的油质蛋白JcOle14.3。JcOle14.3理论等电点为9.65,正电荷残基(Arg+Lys)总数为10个,负电荷残基(Asp+Glu)总数为6个;不稳定系数为25.90,属于稳定性蛋白;其二级结构的主要构件为α-螺旋、随机卷曲和延伸链。根据高级结构预测结果推测JcOle14.3含有N-末端两亲性结构域、中间疏水性结构域以及C-末端两亲性结构域。C-末端两亲性结构域包含1个α-螺旋结构域,中间疏水性结构域包含由3个α-螺旋构成的跨膜结构域,并具有oleosin蛋白特征性的高度保守的脯氨酸结模体,推测在oleosin蛋白与单层磷脂层和油体锚定结合上起着重要的作用。该研究为后期JcOle14.3的异源表达和功能研究奠定了基础。
