已有的资料证明：背根神经节(DRG)神经元膜存在有SP受体(Dray和Pinnoek．1982；司军强和李之望，1995；李之望等，1995；胡宏镇等，1995)以及ATP受雄(Krishtal et al，1982；Bean et al，1990；Li et al。1993；胡宏镇和李之望，1995)...已有的资料证明：背根神经节(DRG)神经元膜存在有SP受体(Dray和Pinnoek．1982；司军强和李之望，1995；李之望等，1995；胡宏镇等，1995)以及ATP受雄(Krishtal et al，1982；Bean et al，1990；Li et al。1993；胡宏镇和李之望，1995)。本文研究的目的是探索SP对ATP受体是否有调制作用。实验在用酶和机械分离的大鼠DRG神经元应用全细胞膜片钳技术记录ATP-激活内向电流，在所检测的85个细胞中绝大多数细胞均对ATP敏感(82／85，96．5％)。展开更多
我室以往的研究证明；在蟾蜍大多数细胞(84%)对谷氨酸敏感，引起膜去极化反应，而免疫组织化学检测有86%的细胞呈谷氨酸样免疫反应性(Glu-LI)，因而我们提出了初级感觉神经元膜可能存在有Glu自身受体的推断(周小萍等1991；李之望1992，1...我室以往的研究证明；在蟾蜍大多数细胞(84%)对谷氨酸敏感，引起膜去极化反应，而免疫组织化学检测有86%的细胞呈谷氨酸样免疫反应性(Glu-LI)，因而我们提出了初级感觉神经元膜可能存在有Glu自身受体的推断(周小萍等1991；李之望1992，1993)。此后日本大阪大学Tohyama实验室(Satoetsl，1993；Tohyama et a1。1994)应用原位杂交及免疫组化实验也提出了同样的推论。展开更多
The roles of sympathicus, sensory neuropeptides (SNP),cyclooxygenase metabolites (COX-M), lipoxygenase metabolites (LOX-M), endothelium derived relaxing factor (EDRF),reactive oxygen (ROS) and potassium channels (PC) ...The roles of sympathicus, sensory neuropeptides (SNP),cyclooxygenase metabolites (COX-M), lipoxygenase metabolites (LOX-M), endothelium derived relaxing factor (EDRF),reactive oxygen (ROS) and potassium channels (PC) in the hypoxic pulmonary vasoconstriction (HPV) and hypoxic cerebral vasodilation (HCVD) were investigated in intact rats, rabbits and dogs. The results showed that during hypoxia,the excitation of sympathicus caused a constriction of both pulmonary and cerebral vessels,while SNP, EDRF and the opening of voltage sensitive PC caused the dilation of both of them; LOX-M mediated HPV and HCVD, COX-M might serve as their modulators; the blockade of ATP sensitive PC induced by hypoxia mediated HPV, but had no effect on HCVD; the reduction of O_2￣- in the lung might potentiate HPV, however, O_2￣- remained unchanged in brain during hypoxia. It is suggested that the alterations of LOX-M,ROS and the ATP sensitive PC are the factors accounting for the difference in the response of pulmonary and cerebral vessels to hypoxia.展开更多
文摘已有的资料证明：背根神经节(DRG)神经元膜存在有SP受体(Dray和Pinnoek．1982；司军强和李之望，1995；李之望等，1995；胡宏镇等，1995)以及ATP受雄(Krishtal et al，1982；Bean et al，1990；Li et al。1993；胡宏镇和李之望，1995)。本文研究的目的是探索SP对ATP受体是否有调制作用。实验在用酶和机械分离的大鼠DRG神经元应用全细胞膜片钳技术记录ATP-激活内向电流，在所检测的85个细胞中绝大多数细胞均对ATP敏感(82／85，96．5％)。
文摘我室以往的研究证明；在蟾蜍大多数细胞(84%)对谷氨酸敏感，引起膜去极化反应，而免疫组织化学检测有86%的细胞呈谷氨酸样免疫反应性(Glu-LI)，因而我们提出了初级感觉神经元膜可能存在有Glu自身受体的推断(周小萍等1991；李之望1992，1993)。此后日本大阪大学Tohyama实验室(Satoetsl，1993；Tohyama et a1。1994)应用原位杂交及免疫组化实验也提出了同样的推论。
文摘The roles of sympathicus, sensory neuropeptides (SNP),cyclooxygenase metabolites (COX-M), lipoxygenase metabolites (LOX-M), endothelium derived relaxing factor (EDRF),reactive oxygen (ROS) and potassium channels (PC) in the hypoxic pulmonary vasoconstriction (HPV) and hypoxic cerebral vasodilation (HCVD) were investigated in intact rats, rabbits and dogs. The results showed that during hypoxia,the excitation of sympathicus caused a constriction of both pulmonary and cerebral vessels,while SNP, EDRF and the opening of voltage sensitive PC caused the dilation of both of them; LOX-M mediated HPV and HCVD, COX-M might serve as their modulators; the blockade of ATP sensitive PC induced by hypoxia mediated HPV, but had no effect on HCVD; the reduction of O_2￣- in the lung might potentiate HPV, however, O_2￣- remained unchanged in brain during hypoxia. It is suggested that the alterations of LOX-M,ROS and the ATP sensitive PC are the factors accounting for the difference in the response of pulmonary and cerebral vessels to hypoxia.
