Background: The prevalence of overweight and obesity associated with oxidative stress and immune abnormalities is continuously increasing. Antioxidant supplementations might counteract potential damage caused by ROS t...Background: The prevalence of overweight and obesity associated with oxidative stress and immune abnormalities is continuously increasing. Antioxidant supplementations might counteract potential damage caused by ROS to cellular tissues. Objective: To determine the role of vitamins on immune improvement during obesity, we investigated in vitro effects of vitamins C, E, and NADH on mitogen-stimulated proliferation, Th1- and Th2-type cytokine production, and oxidant/antioxidant status of lymphocytes isolated from obese patients. Methods: Peripheral blood lymphocytes were isolated using a density gradient of Histopaque. They were in vitro cultured and stimulated by Con A in the presence or absence of vitamins. Cell proliferation was determined by MTT assay and interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions. Cell oxidant/antioxidant balance was studied by assaying glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronucli frequency. Results: Obesity is associated with enhanced oxidative stress response. Indeed, vitamin C, E and NADH improved significantly lymphocyte proliferation and diminished cellular oxidative stress. Conclusion: Treatments of lymphocytes with vitamins had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, generating an anti-inflammatory profile and should be considered in therapeutic approaches for normalizing immune cell function in obesity.展开更多
Diabetes mellitus is a predominant chronic disease which causes mortality of millions of people yearly. Its prevalence is on the rise worldwide. Water kefir is fermented food produced by a matrix of polysaccharides co...Diabetes mellitus is a predominant chronic disease which causes mortality of millions of people yearly. Its prevalence is on the rise worldwide. Water kefir is fermented food produced by a matrix of polysaccharides containing bacteria and yeasts, with therapeutic properties. Our study aimed to evaluate anti-hyperglycemic and anti-hyperlipidemic activities of water kefir on streptozotocin-induced diabetic Wistar rats. Adult Wistar rats were made diabetic by intraperitoneal injection of streptozotocin, and were given or not kefir in drinking water for 5 weeks. Body weight, glucose and lipid levels were measured. The results demonstrated evident improvement in body weight, glucose, and lipid profiles of treated rats comparing with diabetic or control rats. Water kefir is found to be less cost hypoglycemic and hypolipidimic treatment and less time consuming. Water kefir can potentially be useful food for diabetes to control glucose and lipid levels.展开更多
Background: Dietary fatty acids have important homeostatic functions in regulating the immune response and may exert beneficial effects on immune alterations during obesity. Objective: To assess the in vitro effects o...Background: Dietary fatty acids have important homeostatic functions in regulating the immune response and may exert beneficial effects on immune alterations during obesity. Objective: To assess the in vitro effects of oil fatty acids, different oils (olive, linseed, Nigel, sunflower) were tested on T-lymphocyte proliferation, Th1- and Th2-type cytokine production, and intracellular oxidant/antioxidant status in obese patients. Methods: Peripheral blood lymphocytes were isolated using Histopaque and were in vitro cultured and stimulated by Con A in the presence or absence of the oils. Cell proliferation, interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions and intracellular oxidative status (glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronuclei frequency) were investigated. Results: Abnormalities in lymphocyte function and intracellular oxidative stress were observed in obesity. Linseed oil induced a reduction in T-lymphocyte proliferation and IL-2 production while Nigel oil increased them in both obese and control groups. In addition, Nigel oil enhanced IFNγ and IL-4 secretion. Olive and sunflower oils had no effect on lymphocyte proliferation and cytokine secretion in both groups. Linseed and Nigel oils induced an increase in T cell GSH concentrations and catalase activity with a concomitant decrease in MDA, carbonyl protein contents and micronuclei frequency especially in obese patients. Conclusion: Linseed and Nigel oils had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, while olive and sunflower oils had no effects on immune cell function in obesity.展开更多
Antioxidants such as vitamin C and NADH can protect against pesticide adverse effects on immune function. The aim of this work was to study the in vitro effects of vitamin C and NADH on the proliferative responses of ...Antioxidants such as vitamin C and NADH can protect against pesticide adverse effects on immune function. The aim of this work was to study the in vitro effects of vitamin C and NADH on the proliferative responses of human lymphocytes exposed to pesticides （Mancozeb fungicide and Metribuzin herbicide） and on Thl and Th2 cytokine secretion. Their possible protective role on intracellular stress oxidative induced by pesticides was also investigated. Peripheral blood lymphocytes were isolated using differential centrifugation on a density gradient of Histopaque. They were cultured with mitogen concanavalin A （Con A）, Mancozeb and Metribuzin （50 μM） in the presence or the absence of vitamin C （50 μM） or NADH （50 μM）. Proliferation （MTT assay）, IL-2, INFγ and IL-4 （Elisa kits）, oxidative markers （intracellular superoxide anion, hydroperoxides, carbonyl proteins, GSH, catalase and SOD） were determined, The results showed that pesticides were immunosuppressive and decreased cytokine secretion with a shift away from to Thl phenotype. These immunomodulatory properties were accompanied by an increase in lymphocyte intracellular oxidative stress. The presence of vitamin C or NADH, in the medium, exhibited protective effects in human lymphocytes by inhibiting pesticide---induced lymphocyte proliferation suppression, inflammatory status and oxidative stress generation. In conclusion, vitamin C and NADH can result in a safe and effective method to reduce pesticide adverse effects and help to restore immune function.展开更多
文摘Background: The prevalence of overweight and obesity associated with oxidative stress and immune abnormalities is continuously increasing. Antioxidant supplementations might counteract potential damage caused by ROS to cellular tissues. Objective: To determine the role of vitamins on immune improvement during obesity, we investigated in vitro effects of vitamins C, E, and NADH on mitogen-stimulated proliferation, Th1- and Th2-type cytokine production, and oxidant/antioxidant status of lymphocytes isolated from obese patients. Methods: Peripheral blood lymphocytes were isolated using a density gradient of Histopaque. They were in vitro cultured and stimulated by Con A in the presence or absence of vitamins. Cell proliferation was determined by MTT assay and interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions. Cell oxidant/antioxidant balance was studied by assaying glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronucli frequency. Results: Obesity is associated with enhanced oxidative stress response. Indeed, vitamin C, E and NADH improved significantly lymphocyte proliferation and diminished cellular oxidative stress. Conclusion: Treatments of lymphocytes with vitamins had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, generating an anti-inflammatory profile and should be considered in therapeutic approaches for normalizing immune cell function in obesity.
文摘Diabetes mellitus is a predominant chronic disease which causes mortality of millions of people yearly. Its prevalence is on the rise worldwide. Water kefir is fermented food produced by a matrix of polysaccharides containing bacteria and yeasts, with therapeutic properties. Our study aimed to evaluate anti-hyperglycemic and anti-hyperlipidemic activities of water kefir on streptozotocin-induced diabetic Wistar rats. Adult Wistar rats were made diabetic by intraperitoneal injection of streptozotocin, and were given or not kefir in drinking water for 5 weeks. Body weight, glucose and lipid levels were measured. The results demonstrated evident improvement in body weight, glucose, and lipid profiles of treated rats comparing with diabetic or control rats. Water kefir is found to be less cost hypoglycemic and hypolipidimic treatment and less time consuming. Water kefir can potentially be useful food for diabetes to control glucose and lipid levels.
文摘Background: Dietary fatty acids have important homeostatic functions in regulating the immune response and may exert beneficial effects on immune alterations during obesity. Objective: To assess the in vitro effects of oil fatty acids, different oils (olive, linseed, Nigel, sunflower) were tested on T-lymphocyte proliferation, Th1- and Th2-type cytokine production, and intracellular oxidant/antioxidant status in obese patients. Methods: Peripheral blood lymphocytes were isolated using Histopaque and were in vitro cultured and stimulated by Con A in the presence or absence of the oils. Cell proliferation, interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions and intracellular oxidative status (glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronuclei frequency) were investigated. Results: Abnormalities in lymphocyte function and intracellular oxidative stress were observed in obesity. Linseed oil induced a reduction in T-lymphocyte proliferation and IL-2 production while Nigel oil increased them in both obese and control groups. In addition, Nigel oil enhanced IFNγ and IL-4 secretion. Olive and sunflower oils had no effect on lymphocyte proliferation and cytokine secretion in both groups. Linseed and Nigel oils induced an increase in T cell GSH concentrations and catalase activity with a concomitant decrease in MDA, carbonyl protein contents and micronuclei frequency especially in obese patients. Conclusion: Linseed and Nigel oils had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, while olive and sunflower oils had no effects on immune cell function in obesity.
文摘Antioxidants such as vitamin C and NADH can protect against pesticide adverse effects on immune function. The aim of this work was to study the in vitro effects of vitamin C and NADH on the proliferative responses of human lymphocytes exposed to pesticides （Mancozeb fungicide and Metribuzin herbicide） and on Thl and Th2 cytokine secretion. Their possible protective role on intracellular stress oxidative induced by pesticides was also investigated. Peripheral blood lymphocytes were isolated using differential centrifugation on a density gradient of Histopaque. They were cultured with mitogen concanavalin A （Con A）, Mancozeb and Metribuzin （50 μM） in the presence or the absence of vitamin C （50 μM） or NADH （50 μM）. Proliferation （MTT assay）, IL-2, INFγ and IL-4 （Elisa kits）, oxidative markers （intracellular superoxide anion, hydroperoxides, carbonyl proteins, GSH, catalase and SOD） were determined, The results showed that pesticides were immunosuppressive and decreased cytokine secretion with a shift away from to Thl phenotype. These immunomodulatory properties were accompanied by an increase in lymphocyte intracellular oxidative stress. The presence of vitamin C or NADH, in the medium, exhibited protective effects in human lymphocytes by inhibiting pesticide---induced lymphocyte proliferation suppression, inflammatory status and oxidative stress generation. In conclusion, vitamin C and NADH can result in a safe and effective method to reduce pesticide adverse effects and help to restore immune function.
