Flos Abelmoschus manihot has a long history of application.Recent studies show that total flavone of A.manihot(L.)Medic(TFA)has a wide range of pharmacological activity.This article is a review mainly on chemical comp...Flos Abelmoschus manihot has a long history of application.Recent studies show that total flavone of A.manihot(L.)Medic(TFA)has a wide range of pharmacological activity.This article is a review mainly on chemical composition,method of determination and pharmacologic action of TFA,and it raises research and development direction.展开更多
Objective: To study the effects of total flavone of Abelmoschl Manihot L.Medic (TFA) on the function of platelets and to explore its mechanism. Methods: Rat models of artery-veins bypassing thrombus formation were use...Objective: To study the effects of total flavone of Abelmoschl Manihot L.Medic (TFA) on the function of platelets and to explore its mechanism. Methods: Rat models of artery-veins bypassing thrombus formation were used. The platelets of rabbits were collected. Platelet aggregation was induced by collagen and intracellular calcium ion concentration ([Ca 2+ ]i) was assayed by Fura-2 method. Results: TFA (25, 50, 100 mg/kg) significantly and dose-dependently reduced the weight of thrombus. TFA (0.025, 0.05, 0.1 mg/ml) possessed dose-dependant inhibitory effects on rabbits' platelet aggregation induced by collagen. TFA significantly reduced the resting and CaCl 2-induced increase of free intracellular calcium concentration ([Ca 2+ ]i) in rabbit platelet in vitro . Conclusion: TFA has an antiplatelet effect via the inhibition on the influx of Ca 2+ .展开更多
以菜芙蓉花为原料,采用超声协同乙醇⁃硫酸铵双水相提取黄酮,在单因素试验基础上,通过响应面试验优化提取工艺,并利用超高效液相色谱⁃轨道离子阱质谱(ultra⁃high performance liquid chromatography⁃orbit ion trap mass spectrometry,UP...以菜芙蓉花为原料,采用超声协同乙醇⁃硫酸铵双水相提取黄酮,在单因素试验基础上,通过响应面试验优化提取工艺,并利用超高效液相色谱⁃轨道离子阱质谱(ultra⁃high performance liquid chromatography⁃orbit ion trap mass spectrometry,UPLC⁃Orbitrap⁃MS)进行成分分析,与传统方法提取的黄酮进行抗氧化活性比较,并分析黄酮主要单体与抗氧化活性的相关性。结果表明:菜芙蓉花黄酮最佳提取工艺为乙醇质量分数31%、硫酸铵质量分数20%、超声功率210 W、菜芙蓉花粉末质量0.5 g,在此条件下相比为1.16±0.03,分配系数为50.13±2.50,黄酮提取率达(98.31±0.13)%。UPLC⁃Orbitrap⁃MS鉴定出63个黄酮类化合物,其中13个为首次鉴定出的化合物。与双水相法、超声法和醇提法相比,超声协同双水相提取的菜芙蓉花黄酮显示出较强的抗氧化活性,清除DPPH自由基和ABTS+自由基EC_(50)分别为6.37μg/mL和17.71μg/mL。超声协同双水相法能高效提取菜芙蓉花黄酮,且该黄酮具有较高抗氧化活性。展开更多
Objective To explore the molecular mechanisms of the total flavonoids extracted from the flowers of Abelmoschus manihot（TFA） against α-naphthylisothiocyanate（ANIT）-induced cholestasis. Methods The hepatoprotectiv...Objective To explore the molecular mechanisms of the total flavonoids extracted from the flowers of Abelmoschus manihot（TFA） against α-naphthylisothiocyanate（ANIT）-induced cholestasis. Methods The hepatoprotective activities of TFA（125, 250 and 500 mg/kg） were investigated on ANIT-induced cholestatic liver injury in rats. Serum levels of alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, total bilirubin（TBIL）, total bile acid（TBA）, and bile flow were measured to evaluate the protective effect of TFA. Furthermore, the hepatic m RNA and protein levels of transports, multidrug resistance-associated protein 2（MRP2）, bile salt export pump（BSEP）, and Na＋-taurocholate cotransporting polypeptide（NTCP） were investigated to elucidate the protective mechanisms of TFA against ANIT-induced cholestasis. Results Pretreatment of TFA significantly and dose-dependently decreased the ANIT-induced elevation of serum ALT, AST, TBIL, and TBA levels and increased the ANIT-induced suppression of bile flow. Moreover, TFA was found to increase the expression of liver MRP2, BSEP, and NTCP in both protein and m RNA levels in ANIT-induced liver injury in rats with cholestasis. Conclusion TFA exerts a therapeutic effect on ANIT-induced liver injury in rats with cholestasis, possibly through regulating the expressions of hepatic transporters.展开更多
Abelmoschus manihot(L.)Medik.(A.manihot)is a traditional Chinese herbal medicine with a variety of pharmacological properties.It was first recorded in Jiayou Materia Medica dating back to the Song dynasty to eliminate...Abelmoschus manihot(L.)Medik.(A.manihot)is a traditional Chinese herbal medicine with a variety of pharmacological properties.It was first recorded in Jiayou Materia Medica dating back to the Song dynasty to eliminate urinary tract irritation by clearing away heat and diuretic effect.However,its pharmacological action on urinary tract infections has not been investigated.The present study aims to evaluate the anti-inflammatory activity of A.manihot on a mouse model of lipopolysaccharide(LPS)-induced cystitis.The results showed that A.manihot decreased white blood cell(WBC)count in urine sediments of the cystitis mice,alleviated bladder congestion,edema,as well as histopathological damage,reduced the expression levels of tumor necrosis factor-α,interleukin-6,and interleukin-1βsimultaneously.Moreover,A.manihot administration significantly downregulated the expression levels of TLR4,MYD88,IκBα,p-IκBα,NF-κB p65,and p-NF-κB p65 in LPS-induced cystitis mice.These findings demonstrated the protective effect of A.manihot against LPS-induced cystitis,which is attributed to its anti-inflammatory profile by suppressing TLR4/MYD88/NF-κB pathways.Our results suggest that A.manihot could be a potential candidate for cystitis treatment.展开更多
基金Jiangsu Natural Science Foundation Project(BK2008490).
文摘Flos Abelmoschus manihot has a long history of application.Recent studies show that total flavone of A.manihot(L.)Medic(TFA)has a wide range of pharmacological activity.This article is a review mainly on chemical composition,method of determination and pharmacologic action of TFA,and it raises research and development direction.
基金Supported by the Natural Science Fund of Anhui Province (No 99044433) Supported by the Nature and Science of Education Office of Anhui Province (No 2003 KJ 224)
文摘Objective: To study the effects of total flavone of Abelmoschl Manihot L.Medic (TFA) on the function of platelets and to explore its mechanism. Methods: Rat models of artery-veins bypassing thrombus formation were used. The platelets of rabbits were collected. Platelet aggregation was induced by collagen and intracellular calcium ion concentration ([Ca 2+ ]i) was assayed by Fura-2 method. Results: TFA (25, 50, 100 mg/kg) significantly and dose-dependently reduced the weight of thrombus. TFA (0.025, 0.05, 0.1 mg/ml) possessed dose-dependant inhibitory effects on rabbits' platelet aggregation induced by collagen. TFA significantly reduced the resting and CaCl 2-induced increase of free intracellular calcium concentration ([Ca 2+ ]i) in rabbit platelet in vitro . Conclusion: TFA has an antiplatelet effect via the inhibition on the influx of Ca 2+ .
文摘以菜芙蓉花为原料,采用超声协同乙醇⁃硫酸铵双水相提取黄酮,在单因素试验基础上,通过响应面试验优化提取工艺,并利用超高效液相色谱⁃轨道离子阱质谱(ultra⁃high performance liquid chromatography⁃orbit ion trap mass spectrometry,UPLC⁃Orbitrap⁃MS)进行成分分析,与传统方法提取的黄酮进行抗氧化活性比较,并分析黄酮主要单体与抗氧化活性的相关性。结果表明:菜芙蓉花黄酮最佳提取工艺为乙醇质量分数31%、硫酸铵质量分数20%、超声功率210 W、菜芙蓉花粉末质量0.5 g,在此条件下相比为1.16±0.03,分配系数为50.13±2.50,黄酮提取率达(98.31±0.13)%。UPLC⁃Orbitrap⁃MS鉴定出63个黄酮类化合物,其中13个为首次鉴定出的化合物。与双水相法、超声法和醇提法相比,超声协同双水相提取的菜芙蓉花黄酮显示出较强的抗氧化活性,清除DPPH自由基和ABTS+自由基EC_(50)分别为6.37μg/mL和17.71μg/mL。超声协同双水相法能高效提取菜芙蓉花黄酮,且该黄酮具有较高抗氧化活性。
基金National Nature Science Foundation of China(No.30572350)New Drug Foundation of State Administration of Traditional Chinese Medicine(DIX005A)
文摘Objective To explore the molecular mechanisms of the total flavonoids extracted from the flowers of Abelmoschus manihot（TFA） against α-naphthylisothiocyanate（ANIT）-induced cholestasis. Methods The hepatoprotective activities of TFA（125, 250 and 500 mg/kg） were investigated on ANIT-induced cholestatic liver injury in rats. Serum levels of alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, total bilirubin（TBIL）, total bile acid（TBA）, and bile flow were measured to evaluate the protective effect of TFA. Furthermore, the hepatic m RNA and protein levels of transports, multidrug resistance-associated protein 2（MRP2）, bile salt export pump（BSEP）, and Na＋-taurocholate cotransporting polypeptide（NTCP） were investigated to elucidate the protective mechanisms of TFA against ANIT-induced cholestasis. Results Pretreatment of TFA significantly and dose-dependently decreased the ANIT-induced elevation of serum ALT, AST, TBIL, and TBA levels and increased the ANIT-induced suppression of bile flow. Moreover, TFA was found to increase the expression of liver MRP2, BSEP, and NTCP in both protein and m RNA levels in ANIT-induced liver injury in rats with cholestasis. Conclusion TFA exerts a therapeutic effect on ANIT-induced liver injury in rats with cholestasis, possibly through regulating the expressions of hepatic transporters.
基金supported by the“Double First-Class”University project(CPU2018GF06).
文摘Abelmoschus manihot(L.)Medik.(A.manihot)is a traditional Chinese herbal medicine with a variety of pharmacological properties.It was first recorded in Jiayou Materia Medica dating back to the Song dynasty to eliminate urinary tract irritation by clearing away heat and diuretic effect.However,its pharmacological action on urinary tract infections has not been investigated.The present study aims to evaluate the anti-inflammatory activity of A.manihot on a mouse model of lipopolysaccharide(LPS)-induced cystitis.The results showed that A.manihot decreased white blood cell(WBC)count in urine sediments of the cystitis mice,alleviated bladder congestion,edema,as well as histopathological damage,reduced the expression levels of tumor necrosis factor-α,interleukin-6,and interleukin-1βsimultaneously.Moreover,A.manihot administration significantly downregulated the expression levels of TLR4,MYD88,IκBα,p-IκBα,NF-κB p65,and p-NF-κB p65 in LPS-induced cystitis mice.These findings demonstrated the protective effect of A.manihot against LPS-induced cystitis,which is attributed to its anti-inflammatory profile by suppressing TLR4/MYD88/NF-κB pathways.Our results suggest that A.manihot could be a potential candidate for cystitis treatment.
