The transgene technology showed a potentiali-ty in crop improvement such as disease and in-sect resistance,anti-adversity,and grain qual-ity.The inheritance of bar gene for herbicideBASTA resistance in stable transfor...The transgene technology showed a potentiali-ty in crop improvement such as disease and in-sect resistance,anti-adversity,and grain qual-ity.The inheritance of bar gene for herbicideBASTA resistance in stable transformed ricelines was studied for an understanding of theforeign gene inheritance pattern. BASTA resistant transgenic rice plantletswere regenerated from the embryos of rice va-rieties JY119 bombarded with bar gene.A sta-ble BASTA resistant TRline was consecutiv-展开更多
[Objective]The aim was to study the inheritance property of the color of seed in black wheat.[Method]Segregation for seed coat colors was studied in F1,F2 and F3 population from eight crosses of four black-seed wheat ...[Objective]The aim was to study the inheritance property of the color of seed in black wheat.[Method]Segregation for seed coat colors was studied in F1,F2 and F3 population from eight crosses of four black-seed wheat cultivars（Heixiaomai76,Heixiaomai18,96-45 and S-1）with four white-seed wheat cultivars（9-231,Ningchun16,Ningchun17 and Xinchun22）.[Result]The results showed that the color of Heixiaomai 76 maternal genetic traits is controlled by two incompletely dominant genes,the segregation in F3 populations fits 9（dark）∶7（white）ratio.Color of S-1,96-45,and Heixiaomai 18 is controlled by two Independent and complementary genetic genes,the segregation for black kernel color in F2 populations fits 9（dark）∶7（white）ratio.[Conclusion]Different kinds of new species can be breed through the hybrid of black wheat and white wheat varieties.展开更多
Bar gene driven under the control of CaMV35s promotor was delivered into the immatureembroys of a japonica rice cultivar Jingying 119through biolistic approach. Two putativetransgenic plants were produced, which ex-pr...Bar gene driven under the control of CaMV35s promotor was delivered into the immatureembroys of a japonica rice cultivar Jingying 119through biolistic approach. Two putativetransgenic plants were produced, which ex-pressed Basta-resistance. One of the Basta-re-sistance transgenic rices was completely sterile(JY 119-3), the other was self-fertile (JY119-4).展开更多
We have recently developed a systematic method for the study on the inheritance of resistance to sheath blight. The key of the system is an innovated method of inoculation and investigation along with the employment o...We have recently developed a systematic method for the study on the inheritance of resistance to sheath blight. The key of the system is an innovated method of inoculation and investigation along with the employment of the permanent population. This paper reported the procedure of the system and the result of its verification.展开更多
Two sympatric populations of brown planthopper （BPH）, one from rice and the other from Leersia hexandra were collected from each of five locations in Malaysia. All the tested malathion-resistant individuals of the r...Two sympatric populations of brown planthopper （BPH）, one from rice and the other from Leersia hexandra were collected from each of five locations in Malaysia. All the tested malathion-resistant individuals of the rice BPH population and F1 generation （cross between malathion-resistant [usually caught on rice] and malathion- susceptible [usually caught on Leersia]） showed high esterase activity, while all malathion- susceptible individuals on L. hexandra showed low esterase activity. In the F2 generation, all the individuals tested against malathion were approximately 75% resistant and 25% susceptible and the inheritance pattern of esterase activity （high and low esterase activity） segregated in the same manner to a 3：1 ratio. This confirms that resistance to malathion is mono-factorial and inheritance pattern ofesterase activity is also linked to malathion resistance. Carboxylesterase or total esterase activity in BPH is inherited in a simple Mendelian fashion that is encoded by a single dominant gene. For the total esterase assay, average esterase activity levels in the rice-infesting population ranged from 17.64 to 19.37 nmoles 1-napthol/mg protein while that in the Leersia-infesting population ranged from 5.29 to 6.11 nmoles 1-napthol/mg protein. In terms of esterase activity, the two sympatric Nilaparvata lugens populations separated into two distinct groups. Results based on the tube color intensity test showed 96% and 98% resistant and susceptible individuals were present in the rice- and Leersia-infesting populations, respectively. In a filter paper test, the rice-infesting population had 94% with high esterase activity while the Leersia-infesting population had 96% with low esterase activity.展开更多
文摘The transgene technology showed a potentiali-ty in crop improvement such as disease and in-sect resistance,anti-adversity,and grain qual-ity.The inheritance of bar gene for herbicideBASTA resistance in stable transformed ricelines was studied for an understanding of theforeign gene inheritance pattern. BASTA resistant transgenic rice plantletswere regenerated from the embryos of rice va-rieties JY119 bombarded with bar gene.A sta-ble BASTA resistant TRline was consecutiv-
基金Supported by the "Eleventh-Five"Breeding Critical Program of Xin-jiang Production and Construction Corps(2006GG04)Science and Technology Start-up Fund of Higher Layer Personnel of Shihezi University(RCZX200720)~~
文摘[Objective]The aim was to study the inheritance property of the color of seed in black wheat.[Method]Segregation for seed coat colors was studied in F1,F2 and F3 population from eight crosses of four black-seed wheat cultivars（Heixiaomai76,Heixiaomai18,96-45 and S-1）with four white-seed wheat cultivars（9-231,Ningchun16,Ningchun17 and Xinchun22）.[Result]The results showed that the color of Heixiaomai 76 maternal genetic traits is controlled by two incompletely dominant genes,the segregation in F3 populations fits 9（dark）∶7（white）ratio.Color of S-1,96-45,and Heixiaomai 18 is controlled by two Independent and complementary genetic genes,the segregation for black kernel color in F2 populations fits 9（dark）∶7（white）ratio.[Conclusion]Different kinds of new species can be breed through the hybrid of black wheat and white wheat varieties.
文摘Bar gene driven under the control of CaMV35s promotor was delivered into the immatureembroys of a japonica rice cultivar Jingying 119through biolistic approach. Two putativetransgenic plants were produced, which ex-pressed Basta-resistance. One of the Basta-re-sistance transgenic rices was completely sterile(JY 119-3), the other was self-fertile (JY119-4).
文摘We have recently developed a systematic method for the study on the inheritance of resistance to sheath blight. The key of the system is an innovated method of inoculation and investigation along with the employment of the permanent population. This paper reported the procedure of the system and the result of its verification.
文摘Two sympatric populations of brown planthopper （BPH）, one from rice and the other from Leersia hexandra were collected from each of five locations in Malaysia. All the tested malathion-resistant individuals of the rice BPH population and F1 generation （cross between malathion-resistant [usually caught on rice] and malathion- susceptible [usually caught on Leersia]） showed high esterase activity, while all malathion- susceptible individuals on L. hexandra showed low esterase activity. In the F2 generation, all the individuals tested against malathion were approximately 75% resistant and 25% susceptible and the inheritance pattern of esterase activity （high and low esterase activity） segregated in the same manner to a 3：1 ratio. This confirms that resistance to malathion is mono-factorial and inheritance pattern ofesterase activity is also linked to malathion resistance. Carboxylesterase or total esterase activity in BPH is inherited in a simple Mendelian fashion that is encoded by a single dominant gene. For the total esterase assay, average esterase activity levels in the rice-infesting population ranged from 17.64 to 19.37 nmoles 1-napthol/mg protein while that in the Leersia-infesting population ranged from 5.29 to 6.11 nmoles 1-napthol/mg protein. In terms of esterase activity, the two sympatric Nilaparvata lugens populations separated into two distinct groups. Results based on the tube color intensity test showed 96% and 98% resistant and susceptible individuals were present in the rice- and Leersia-infesting populations, respectively. In a filter paper test, the rice-infesting population had 94% with high esterase activity while the Leersia-infesting population had 96% with low esterase activity.
