Objective： To explore the mechanism of acupuncture in treating brain aging. Methods： Forty healthy Wistar rats were randomly divided into 4 groups with 10 rats in each group. The rats in the control group didn＇t re...Objective： To explore the mechanism of acupuncture in treating brain aging. Methods： Forty healthy Wistar rats were randomly divided into 4 groups with 10 rats in each group. The rats in the control group didn＇t received modeling and treatment. The rats in the model group, the Governor Vessel acupoints group （GVA group） and the body acupoints group （BA group） were induced as subacute aging rat model. The rats in the model group didn＇t take treatment. On the modeling day, the rats in the GVA group received acupuncture at Baihui （GV 20） and Dazhui （GV 14） while the rats in the BA group received acupuncture at Guanyuan （CV 3） and Zusanli （ST 36）. The contents of NO and NOS, as well as the activity of Ca^2＋-Mg^2＋-ATPase in the brain tissue of rats in each group were observed. Results： After modeling, the contents of NO and NOS of brain tissue increased significantly and the activity of Ca2^＋-Mg^2＋-ATPase decreased markedly. Acupuncture treatment could inhibit the increases of NO and NOS contents, improve the decrease of the activity of Ca^2＋-Mg^2＋-ATPase. The results of the GVA group were better than those of BA group （both P〈0.05）. Conclusion： Acupuncture improved the activity of Ca^2＋-Mg^2＋-ATPase and stabilized the NOS concentration. As a result, the NO content was balanced and the ohvsiological function of NO was fully induced for anti-a^in~, esoeciallv in the GVA group.展开更多
Primary cultures of rat cortical neurons were treated with H2O2 in an in vitro model of free radical neurotoxicity. Flavonoids extracted from the stems and leaves of Scutellaria baicalensis Georgi, known as SSF, at co...Primary cultures of rat cortical neurons were treated with H2O2 in an in vitro model of free radical neurotoxicity. Flavonoids extracted from the stems and leaves of Scutellaria baicalensis Georgi, known as SSF, at concentrations of 18.98, 37.36 and 75.92 μg/mL, protected neurons against H2O2 injury in a dose-dependent manner. SSF increased cell survival, reduced lactate dehydrogenase release and inhibited malondialdehyde production. SSF also inhibited reductions in superoxide dismutase, glutathione peroxidase and Na＋-K＋-ATPase activities. These results in-dicate that SSF can protect rat cortical neurons against H2O2-induced oxidative injury.展开更多
基金supported by the Science and Technology Research Project of the Department of Education of Heilongjiang Province(11551449)
文摘Objective： To explore the mechanism of acupuncture in treating brain aging. Methods： Forty healthy Wistar rats were randomly divided into 4 groups with 10 rats in each group. The rats in the control group didn＇t received modeling and treatment. The rats in the model group, the Governor Vessel acupoints group （GVA group） and the body acupoints group （BA group） were induced as subacute aging rat model. The rats in the model group didn＇t take treatment. On the modeling day, the rats in the GVA group received acupuncture at Baihui （GV 20） and Dazhui （GV 14） while the rats in the BA group received acupuncture at Guanyuan （CV 3） and Zusanli （ST 36）. The contents of NO and NOS, as well as the activity of Ca^2＋-Mg^2＋-ATPase in the brain tissue of rats in each group were observed. Results： After modeling, the contents of NO and NOS of brain tissue increased significantly and the activity of Ca2^＋-Mg^2＋-ATPase decreased markedly. Acupuncture treatment could inhibit the increases of NO and NOS contents, improve the decrease of the activity of Ca^2＋-Mg^2＋-ATPase. The results of the GVA group were better than those of BA group （both P〈0.05）. Conclusion： Acupuncture improved the activity of Ca^2＋-Mg^2＋-ATPase and stabilized the NOS concentration. As a result, the NO content was balanced and the ohvsiological function of NO was fully induced for anti-a^in~, esoeciallv in the GVA group.
基金the State Administration of Traditional Chinese Medicine, People’s Republic of China, No.02-03-ZP18Hebei Provincial Education Department,No. 20015Hebei Provincial Hundred Outstanding Innovated Talents, First Batch
文摘Primary cultures of rat cortical neurons were treated with H2O2 in an in vitro model of free radical neurotoxicity. Flavonoids extracted from the stems and leaves of Scutellaria baicalensis Georgi, known as SSF, at concentrations of 18.98, 37.36 and 75.92 μg/mL, protected neurons against H2O2 injury in a dose-dependent manner. SSF increased cell survival, reduced lactate dehydrogenase release and inhibited malondialdehyde production. SSF also inhibited reductions in superoxide dismutase, glutathione peroxidase and Na＋-K＋-ATPase activities. These results in-dicate that SSF can protect rat cortical neurons against H2O2-induced oxidative injury.
