To resolve the ontology understanding problem, the structural features and the potential important terms of a large-scale ontology are investigated from the perspective of complex networks analysis. Through the empiri...To resolve the ontology understanding problem, the structural features and the potential important terms of a large-scale ontology are investigated from the perspective of complex networks analysis. Through the empirical studies of the gene ontology with various perspectives, this paper shows that the whole gene ontology displays the same topological features as complex networks including ＂small world＂ and ＂scale-free＂,while some sub-ontologies have the ＂scale-free＂ property but no ＂small world＂ effect.The potential important terms in an ontology are discovered by some famous complex network centralization methods.An evaluation method based on information retrieval in MEDLINE is designed to measure the effectiveness of the discovered important terms.According to the relevant literature of the gene ontology terms,the suitability of these centralization methods for ontology important concepts discovering is quantitatively evaluated.The experimental results indicate that the betweenness centrality is the most appropriate method among all the evaluated centralization measures.展开更多
This study employs causal inference methods to analyze user behavior on short-video platforms,examining how content characteristics,algorithmic recommendations,and social networks impact engagement.Using Propensity Sc...This study employs causal inference methods to analyze user behavior on short-video platforms,examining how content characteristics,algorithmic recommendations,and social networks impact engagement.Using Propensity Score Matching(PSM),Regression Discontinuity Design(RDD),and Instrumental Variables(IV),findings indicate that algorithmic promotion significantly boosts content diffusion,emotionally charged content is more shareable than neutral content,and influencer interactions increase visibility by 80%.The study shows that platform algorithms shape both information flow and group psychology.The results offer insights for social media marketing,public opinion management,and digital governance,with policy recommendations for content diversity,platform transparency,and algorithm fairness.展开更多
Immune changes and inflammatory responses have been identified as central events in the pathological process of spinal co rd injury.They can greatly affect nerve regeneration and functional recovery.However,there is s...Immune changes and inflammatory responses have been identified as central events in the pathological process of spinal co rd injury.They can greatly affect nerve regeneration and functional recovery.However,there is still limited understanding of the peripheral immune inflammato ry response in spinal cord inju ry.In this study.we obtained microRNA expression profiles from the peripheral blood of patients with spinal co rd injury using high-throughput sequencing.We also obtained the mRNA expression profile of spinal cord injury patients from the Gene Expression Omnibus(GEO)database(GSE151371).We identified 54 differentially expressed microRNAs and 1656 diffe rentially expressed genes using bioinformatics approaches.Functional enrichment analysis revealed that various common immune and inflammation-related signaling pathways,such as neutrophil extracellular trap formation pathway,T cell receptor signaling pathway,and nuclear factor-κB signal pathway,we re abnormally activated or inhibited in spinal cord inju ry patient samples.We applied an integrated strategy that combines weighted gene co-expression network analysis,LASSO logistic regression,and SVM-RFE algorithm and identified three biomarke rs associated with spinal cord injury:ANO10,BST1,and ZFP36L2.We verified the expression levels and diagnostic perfo rmance of these three genes in the original training dataset and clinical samples through the receiver operating characteristic curve.Quantitative polymerase chain reaction results showed that ANO20 and BST1 mRNA levels were increased and ZFP36L2 mRNA was decreased in the peripheral blood of spinal cord injury patients.We also constructed a small RNA-mRNA interaction network using Cytoscape.Additionally,we evaluated the proportion of 22 types of immune cells in the peripheral blood of spinal co rd injury patients using the CIBERSORT tool.The proportions of naive B cells,plasma cells,monocytes,and neutrophils were increased while the proportions of memory B cells,CD8^(+)T cells,resting natural killer cells,resting dendritic cells,and eosinophils were markedly decreased in spinal cord injury patients increased compared with healthy subjects,and ANO10,BST1 and ZFP26L2we re closely related to the proportion of certain immune cell types.The findings from this study provide new directions for the development of treatment strategies related to immune inflammation in spinal co rd inju ry and suggest that ANO10,BST2,and ZFP36L2 are potential biomarkers for spinal cord injury.The study was registe red in the Chinese Clinical Trial Registry(registration No.ChiCTR2200066985,December 12,2022).展开更多
This paper investigates the uplink throughput of Cognitive Radio Cellular Networks(CRCNs).As oppose to traditional performance evaluation schemes which mainly adopt complex system level simulations,we use the theoreti...This paper investigates the uplink throughput of Cognitive Radio Cellular Networks(CRCNs).As oppose to traditional performance evaluation schemes which mainly adopt complex system level simulations,we use the theoretical framework of stochastic geometry to provide a tractable and accurate analysis of the uplink throughput in the CRCN.By modelling the positions of User Equipments(UEs)and Base Stations(BSs)as Poisson Point Processes(PPPs),we analyse and derive expressions for the link rate and the cell throughput in the Primary(PR)and Secondary(SR)networks.The expressions show that the throughput of the CRCN is mainly affected by the density ratios between the UEs and the BSs in both the PR and SR networks.Besides,a comparative analysis of the link rate between random and regular BS deployments is concluded,and the results confirm the accuracy of our analysis.Furthermore,we define the cognitive throughput gain and derive an expression which is dominated by the traffic load in the PR network.展开更多
BACKGROUND Non-suicidal self-injury(NSSI)is common among adolescents and frequently cooccurs with depression.Understanding the distinct patterns of NSSI behaviors,along with their associated risk and protective factor...BACKGROUND Non-suicidal self-injury(NSSI)is common among adolescents and frequently cooccurs with depression.Understanding the distinct patterns of NSSI behaviors,along with their associated risk and protective factors,is crucial for developing effective interventions.AIM To classify NSSI behaviors and examine interactions between risk and resilience factors in Chinese adolescents.METHODS A cross-sectional study involving 3967 Chinese students(51.7%female,mean age 13.58±2.24 years)who completed questionnaires on parenting styles,bullying,childhood maltreatment,depression,resilience,and NSSI.Latent profile analysis(LPA)was used to identify NSSI subtypes,and network analysis explored interactions between risk and resilience factors.RESULTS Three NSSI subtypes were identified:NSSI with depression(18.8%),NSSI without depression(12.3%),and neither(68.9%).Bullying was the central risk factor across subtypes,while emotional control and family support were key protective factors.Statistical analyses showed significant differences between groups(P<0.001).CONCLUSION This study identified three NSSI subtypes among Chinese adolescents.Bullying emerged as a central risk factor,while emotional control and family support were key protective factors.Targeting these areas may help reduce NSSI behaviors in this population.展开更多
Content analysis of scientific papers emanating from Antarctic science research during the 25 years period （1980-- 2004） has been carried out using neural network based algorithm-CATPAC. A total of 10 942 research a...Content analysis of scientific papers emanating from Antarctic science research during the 25 years period （1980-- 2004） has been carried out using neural network based algorithm-CATPAC. A total of 10 942 research articles published in Science Citation Indexed （SCI） journals were used for the study. Normalized co-word matrix from 35 most-used significant words was used to study the semantic association between the words. Structural Equivalence blocks were constructed from these 35 most-used words. Four-block model solution was found to be optimum. The density table was dichotomized using the mean density of the table to derive the binary matrix, which was used to construct the network map. Network maps represent the thematic character of the blocks. The blocks showed preferred connection in establishing semantic relationship with the blocks, characterizing thematic composition of Antarctic science research. The analysis has provided an analytical framework for carrying out studies on the con- tent of scientific articles. The paper has shown the utility of co-word analysis in highlighting the important areas of research in Antarctic science.展开更多
Fractal and self similarity of complex networks have attracted much attention in recent years. The fractal dimension is a useful method to describe the fractal property of networks. However, the fractal features of mo...Fractal and self similarity of complex networks have attracted much attention in recent years. The fractal dimension is a useful method to describe the fractal property of networks. However, the fractal features of mobile social networks （MSNs） are inadequately investigated. In this work, a box-covering method based on the ratio of excluded mass to closeness centrality is presented to investigate the fractal feature of MSNs. Using this method, we find that some MSNs are fractal at different time intervals. Our simulation results indicate that the proposed method is available for analyzing the fractal property of MSNs.展开更多
An array composed of sixteen gas sensors was constructed to analyze gas mixtures quantitatively. The data of responses from the sensor array to ethane, propane and propylene were treated by three-layer ANN with BP alg...An array composed of sixteen gas sensors was constructed to analyze gas mixtures quantitatively. The data of responses from the sensor array to ethane, propane and propylene were treated by three-layer ANN with BP algorithms and PLS. The analytical results indicated that the concentration predicted with ANN is better than that with PLS. The average prediction errors for ethane, propane and propylene were 5.11%, 8.28%, 2.64%, respectively.展开更多
Objective:Network analysis was used to explore the complex inter-relationships between social participation activities and depressive symptoms among the Chinese older population,and the differences in network structur...Objective:Network analysis was used to explore the complex inter-relationships between social participation activities and depressive symptoms among the Chinese older population,and the differences in network structures among different genders,age groups,and urban-rural residency would be compared.Methods:Based on the 2018 wave of the Chinese Longitudinal Healthy Longevity Survey(CLHLS),12,043 people aged 65 to 105 were included.The 10-item Center for Epidemiologic Studies Depression(CESD)Scale was used to assess depressive symptoms and 10 types of social participation activities were collected,including housework,tai-chi,square dancing,visiting and interacting with friends,garden work,reading newspapers or books,raising domestic animals,playing cards or mahjong,watching TV or listening to radio,and organized social activities.R 4.2.1 software was used to estimate the network model and calculate strength and bridge strength.Results:21.60%(2,601/12,043)of the participants had depressive symptoms.The total social participation score was negatively associated with depressive symptoms after adjusting for sociodemographic factors.The network of social participation and depressive symptoms showed that“D9(Inability to get going)”and“S9(Watching TV and/or listening to the radio)”had the highest strength within depressive symptoms and social participation communities,respectively,and“S1(Housework)”,“S9(Watching TV and/or listening to the radio)”,and“D5(Hopelessness)”were the most prominent bridging nodes between the two communities.Most edges linking the two communities were negative.“S5(Graden work)-D5(Hopelessness)”and“S6(Reading newspapers/books)-D4(Everything was an effort)”were the top 2 strongest negative edges.Older females had significantly denser network structures than older males.Compared to older people aged 65e80,the age group 81e105 showed higher network global strength.Conclusions:This study provides novel insights into the complex relationships between social participation and depressive symptoms.Except for doing housework,other social participation activities were found to be protective for depression levels.Different nursing strategies should be taken to prevent and alleviate depressive symptoms for different genders and older people of different ages.展开更多
Purpose:We analyzed the structure of a community of authors working in the field of social network analysis(SNA)based on citation indicators:direct citation and bibliographic coupling metrics.We observed patterns at t...Purpose:We analyzed the structure of a community of authors working in the field of social network analysis(SNA)based on citation indicators:direct citation and bibliographic coupling metrics.We observed patterns at the micro,meso,and macro levels of analysis.Design/methodology/approach:We used bibliometric network analysis,including the“temporal quantities”approach proposed to study temporal networks.Using a two-mode network linking publications with authors and a one-mode network of citations between the works,we constructed and analyzed the networks of citation and bibliographic coupling among authors.We used an iterated saturation data collection approach.Findings:At the macro-level,we observed the global structural features of citations between authors,showing that 80%of authors have not more than 15 citations from other works.At the meso-level,we extracted the groups of authors citing each other and similar to each other according to their citation patterns.We have seen a division of authors in SNA into groups of social scientists and physicists,as well as into other groups of authors from different disciplines.We found some examples of brokerage between different groups that maintained the common identity of the field.At the micro-level,we extracted authors with extremely high values of received citations,who can be considered as the most prominent authors in the field.We examined the temporal properties of the most popular authors.Research limitations:The main challenge in this approach is the resolution of the author’s name(synonyms and homonyms).We faced the author disambiguation,or“multiple personalities”(Harzing,2015)problem.To remain consistent and comparable with our previously published articles,we used the same SNA data collected up to 2018.The analysis and conclusions on the activity,productivity,and visibility of the authors are relative only to the field of SNA.Practical implications:The proposed approach can be utilized for similar objectives and identifying key structures and characteristics in other disciplines.This may potentially inspire the application of network approaches in other research areas,creating more authors collaborating in the field of SNA.Originality/value:We identified and applied an innovative approach and methods to study the structure of scientific communities,which allowed us to get the findings going beyond those obtained with other methods.We used a new approach to temporal network analysis,which is an important addition to the analysis as it provides detailed information on different measures for the authors and pairs of authors over time.展开更多
The Chinese crested duck is a unique duck breed having a bulbous feather shape on its duck head.However,the mechanisms involved in its formation and development are unclear.In the present study,RNA sequencing analysis...The Chinese crested duck is a unique duck breed having a bulbous feather shape on its duck head.However,the mechanisms involved in its formation and development are unclear.In the present study,RNA sequencing analysis was performed on the crested tissues of 6 Chinese crested ducks and the scalp tissues of 6 cherry valley ducks(CVs)from 2 developmental stages.This study identified 261 differentially expressed genes(DEGs),122 upregulated and 139 downregulated,in the E28 stage and 361 DEGs,154 upregulated and 207 downregulated in the D42 stage between CC and CV ducks.The subsequent results of weighted gene co-expression network analysis(WGCNA)revealed that the turquoise and cyan modules were associated with the crest trait in the D42 stage,meanwhile,the green,brown,and pink modules were associated with the crest trait in the E28 stage.Venn analysis of the DEGs and WGCNA showed that 145 and 45 genes are associated between the D42 and E28 stages,respectively.The expression of WNT16,BMP2,SLC35F2,SLC6A15,APOBEC2,ABHD6,TNNC2,MYL1,and TNNI2 were verified by real-time quantitative PCR.This study provides an approach to reveal the molecular mechanisms underlying the crested trait development.展开更多
Data on discrete,isolated attributes of the marine economy are often used in traditional marine economic research.However,as the focus of urban research shifts from internal static attributes to external dynamic linka...Data on discrete,isolated attributes of the marine economy are often used in traditional marine economic research.However,as the focus of urban research shifts from internal static attributes to external dynamic linkages,the importance of marine economic net-work research is beginning to emerge.The construction of the marine economic network in China’s coastal areas is necessary to change the flow of land and sea resources and optimize regional marine economic development.Employing data from headquarters and branches of sea-related A-share listed enterprises to construct the marine economic network in China,we use social network analysis(SNA)to discuss the characteristics of its evolution as of 2010,2015,and 2020 and its governance.The following results were obtained.1)In terms of topological characteristics,the scale of the marine economic network in China’s coastal areas has accelerated and expan-ded,and the connections have become increasingly close;thus,this development has complex network characteristics.2)In terms of spatial structure,the intensity of the connection fluctuates and does not form stable development support;the group structure gradually becomes clear,but the overall pattern is fragmented;there are spatial differences in marine economic agglomeration radiation;the radi-ation effect of the eastern marine economic circle is obvious;and the polarization effect of northern and southern marine economic circles is significant.On this basis,we construct a framework for the governance of a marine economic network with the market,the government,and industry as the three governing bodies.By clarifying the driving factors and building objectives of marine economic network construction,this study aims to foster the high-quality development of China’s marine economy.展开更多
AIM:To conduct a bibliometric analysis of studies on microphthalmos and anophthalmos(M/A),explore research hotspots,and provide information on future research interests in this field to benefit clinicians and research...AIM:To conduct a bibliometric analysis of studies on microphthalmos and anophthalmos(M/A),explore research hotspots,and provide information on future research interests in this field to benefit clinicians and researchers.METHODS:Totally 751 publications related to M/A from the year 2004 to 2023 were collected from the Web of Science Core Collection database.These publications consist of both original and review articles,that are composed in English.The contributions of different countries,institutions,journals,and authors were analyzed,and network analysis was conducted by using Microsoft Excel 2021,VOSviewer,and R Studio to visualize research hotspots.RESULTS:Among all publications included,the highest number of publications came from USA(218,29.03%).China followed with 99 publications(13.18%),and England with 86 publications(11.45%).The publications from the USA had the highest frequency of citations,with 16699 citations,and the highest H-index of 49.The American Journal of Medical Genetics Part A(43,5.73%)published the largest number of papers,and the University of London had the most publications(41,5.46%).The genetic and molecular mechanisms of M/A were still unclear and the clinical intervention for M/A had gained a lot of attention as an emerging area of interest.CONCLUSION:Data have been gathered on the yearly count of published materials and citations,as well as the rise in publication trends,the efficiency of regions or countries,authors,journals,and organizations,along with the high-cited publications in M/A.The recent trend of research has shifted from genetic mechanisms to different clinical phenotypes and corresponding clinical interventions,which can give direction to future research.展开更多
Social Network Theory and methods have emerged as pivotal tools for dissecting intricate interdisciplinary issues in rural communities.This study aims to systematically delineate the application characteristics and tr...Social Network Theory and methods have emerged as pivotal tools for dissecting intricate interdisciplinary issues in rural communities.This study aims to systematically delineate the application characteristics and trends of Social Network Analysis(SNA)in rural community research.Using a twofold approach,we integrate a traditional literature review and CiteSpace bibliometric analysis to assess the application status and evolutionary trends of SNA methods in this context.The key findings include the following:①Chinese research trends:scholars predominantly concentrate on the“three rural”issues(related to agriculture,rural areas,and small-scale farmers)and social support mechanisms for vulnerable rural populations.With policy shifts,rural revitalization,tourism,governance,social trust,and multi-dimensional poverty are poised to emerge as hot topics for the future.For further refinement,we suggest that the application of SNA in rural community research could benefit from content expansion,long-term studies,and innovative modelling techniques.②Research by international scholars has been primarily directed toward the physical and mental health of rural residents,as well as socioeconomic issues.Despite these studies covering a range of typical cases across various nations,a conspicuous lack of thorough,systematic,and prolonged efforts focused on rural community development in specific regions remains.Additionally,health issues affecting rural residents are expected to sustain long-standing and focused international academic attention.This study contributes to a more nuanced understanding of the current applications and potential future directions of SNA in rural community studies,both in China and internationally.展开更多
BACKGROUND Gastrointestinal neoplasm(GN)significantly impact the global cancer burden and mortality,necessitating early detection and treatment.Understanding the evolution and current state of research in this field i...BACKGROUND Gastrointestinal neoplasm(GN)significantly impact the global cancer burden and mortality,necessitating early detection and treatment.Understanding the evolution and current state of research in this field is vital.AIM To conducts a comprehensive bibliometric analysis of publications from 1984 to 2022 to elucidate the trends and hotspots in the GN risk assessment research,focusing on key contributors,institutions,and thematic evolution.METHODS This study conducted a bibliometric analysis of data from the Web of Science Core Collection database using the"bibliometrix"R package,VOSviewer,and CiteSpace.The analysis focused on the distribution of publications,contributions by institutions and countries,and trends in keywords.The methods included data synthesis,network analysis,and visualization of international collaboration networks.RESULTS This analysis of 1371 articles on GN risk assessment revealed a notable evolution in terms of research focus and collaboration.It highlights the United States'critical role in advancing this field,with significant contributions from institutions such as Brigham and Women's Hospital and the National Cancer Institute.The last five years,substantial advancements have been made,representing nearly 45%of the examined literature.Publication rates have dramatically increased,from 20 articles in 2002 to 112 in 2022,reflecting intensified research efforts.This study underscores a growing trend toward interdisciplinary and international collaboration,with the Journal of Clinical Oncology standing out as a key publication outlet.This shift toward more comprehensive and collaborative research methods marks a significant step in addressing GN risks.CONCLUSION This study underscores advancements in GN risk assessment through genetic analyses and machine learning and reveals significant geographical disparities in research emphasis.This calls for enhanced global collaboration and integration of artificial intelligence to improve cancer prevention and treatment accuracy,ultimately enhancing worldwide patient care.展开更多
Chinese Medicine(CM)has been widely used as an important avenue for disease prevention and treatment in China especially in the form of CM prescriptions combining sets of herbs to address patients’symptoms and syndro...Chinese Medicine(CM)has been widely used as an important avenue for disease prevention and treatment in China especially in the form of CM prescriptions combining sets of herbs to address patients’symptoms and syndromes.However,the selection and compatibility of herbs are complex and abstract due to intrinsic relationships between herbal properties and their overall functions.Network analysis is applied to demonstrate the complex relationships between individual herbal efficacy and the overall function of CM prescriptions.To illustrate their connections and correlations,prescription function(PF),prescription herb(PH),and herbal efficacy(HE)intranetworks are proposed based on CM theory to identify relationships between herbs and prescriptions.These three networks are then connected by PF-PH and PH-HE interlayer networks adopting herb dosage to form a multidimensional heterogeneous network,a Prescription-Herb-Function Network(PHFN).The network is applied to 112 classic prescriptions from Treatise on Exogenous Febrile and Miscellaneous Diseases to illustrate the application of PHFN.The PHFN is constructed including 146 functions in PF intra network,89 herbs in the PH intra network,and 163 herbal efficacies in the HE intra network.The results show that herb pairs with synergistic actions have stronger relevance,such as licorice-cassia twig,licorice-Chinese date,fresh ginger-Chinese date,etc.The integration of dosage to the network helps to indicate the main herbs for cluster analysis and automatic formulation.PHFN also reveals the internal relationships between the functions of prescriptions and composed herbal efficacies.展开更多
BACKGROUND Colon cancer is acknowledged as one of the most common malignancies worldwide,ranking third in United States regarding incidence and mortality.Notably,approximately 40%of colon cancer cases harbor oncogenic...BACKGROUND Colon cancer is acknowledged as one of the most common malignancies worldwide,ranking third in United States regarding incidence and mortality.Notably,approximately 40%of colon cancer cases harbor oncogenic KRAS mutations,resulting in the continuous activation of epidermal growth factor receptor signaling.AIM To investigate the key pathogenic genes in KRAS mutant colon cancer holds considerable importance.METHODS Weighted gene co-expression network analysis,in combination with additional bioinformatics analysis,were conducted to screen the key factors driving the progression of KRAS mutant colon cancer.Meanwhile,various in vitro experiments were also conducted to explore the biological function of transglutaminase 2(TGM2).RESULTS Integrated analysis demonstrated that TGM2 acted as an independent prognostic factor for progression-free survival.Immunohistochemical analysis on tissue microarrays revealed that TGM2 was associated with an elevated probability of perineural invasion in patients with KRAS mutant colon cancer.Additionally,biological roles of the key gene TGM2 was also assessed,suggesting that the downregulation of TGM2 attenuated the proliferation,invasion,and migration of the KRAS mutant colon cancer cell line.CONCLUSION This study underscores the potential significance of TGM2 in the progression of KRAS mutant colon cancer.This insight not only offers a theoretical foundation for therapeutic approaches but also highlights the need for additional clinical trials and fundamental research to support our preliminary findings.展开更多
The inter-city linkage heat data provided by Baidu Migration is employed as a characterization of inter-city linkages in order to facilitate the study of the network linkage characteristics and hierarchical structure ...The inter-city linkage heat data provided by Baidu Migration is employed as a characterization of inter-city linkages in order to facilitate the study of the network linkage characteristics and hierarchical structure of urban agglomeration in the Greater Bay Area through the use of social network analysis method.This is the inaugural application of big data based on location services in the study of urban agglomeration network structure,which represents a novel research perspective on this topic.The study reveals that the density of network linkages in the Greater Bay Area urban agglomeration has reached 100%,indicating a mature network-like spatial structure.This structure has given rise to three distinct communities:Shenzhen-Dongguan-Huizhou,Guangzhou-Foshan-Zhaoqing,and Zhuhai-Zhongshan-Jiangmen.Additionally,cities within the Greater Bay Area urban agglomeration play different roles,suggesting that varying development strategies may be necessary to achieve staggered development.The study demonstrates that large datasets represented by LBS can offer novel insights and methodologies for the examination of urban agglomeration network structures,contingent on the appropriate mining and processing of the data.展开更多
Introduction:Osteoarthritis(OA)is still an important health problem,and understanding its pathological mechanisms is essential for its diagnosis and treatment.There is evidence that autophagy may play a role in OA pro...Introduction:Osteoarthritis(OA)is still an important health problem,and understanding its pathological mechanisms is essential for its diagnosis and treatment.There is evidence that autophagy may play a role in OA progression,but the exact mechanism remains unclear.Methods:In this study,we adopted a multi-prong approach to systematically identify the key autophagy-related genes(ARGs)associated with OA.Through weighted gene coexpression network analysis,we initially identified significant gene modules associated with OA.Subsequent differential gene analysis performed on normal and OA specimens.Further analysis later using the MCC algorithm highlighted hub ARGs.These genes were then incorporated into the prediction model of OA.In addition,the expression patterns of these DEGs were verified by in vitro experiments.Results:A total of 104 differentially expressed genes(DEGs)were identified by differential gene analysis,of which 102 were up-regulated and 2 were down-regulated.These differentially expressed genes were closely related to key signaling pathways,such as PI3K-Akt signaling pathway,IL-17 signaling pathway and osteoclast differentiation.Further MCC analysis highlighted 10 hub ARGs,among which ATF3,CYCS,FOXO3,KLF6,NFKBIA and SOCS3 were particularly significant,which were then included in the prediction model of OA,which showed robust prediction ability with an area under the curve of 0.783.In vitro experiments confirmed that the expression pattern of these DEGs was consistent with our prediction.Conclusion:In summary,our comprehensive analysis not only provides new insights into the molecular basis of OA,but also suggests potential biomarkers for its diagnosis.展开更多
基金The National Basic Research Program of China (973Program) (No.2005CB321802)Program for New Century Excellent Talents in University (No.NCET-06-0926)the National Natural Science Foundation of China (No.60873097,90612009)
文摘To resolve the ontology understanding problem, the structural features and the potential important terms of a large-scale ontology are investigated from the perspective of complex networks analysis. Through the empirical studies of the gene ontology with various perspectives, this paper shows that the whole gene ontology displays the same topological features as complex networks including ＂small world＂ and ＂scale-free＂,while some sub-ontologies have the ＂scale-free＂ property but no ＂small world＂ effect.The potential important terms in an ontology are discovered by some famous complex network centralization methods.An evaluation method based on information retrieval in MEDLINE is designed to measure the effectiveness of the discovered important terms.According to the relevant literature of the gene ontology terms,the suitability of these centralization methods for ontology important concepts discovering is quantitatively evaluated.The experimental results indicate that the betweenness centrality is the most appropriate method among all the evaluated centralization measures.
文摘This study employs causal inference methods to analyze user behavior on short-video platforms,examining how content characteristics,algorithmic recommendations,and social networks impact engagement.Using Propensity Score Matching(PSM),Regression Discontinuity Design(RDD),and Instrumental Variables(IV),findings indicate that algorithmic promotion significantly boosts content diffusion,emotionally charged content is more shareable than neutral content,and influencer interactions increase visibility by 80%.The study shows that platform algorithms shape both information flow and group psychology.The results offer insights for social media marketing,public opinion management,and digital governance,with policy recommendations for content diversity,platform transparency,and algorithm fairness.
基金supported by the Notional Natural Science Foundation of China,No.81960417 (to JX)Guangxi Key Research and Development Program,No.GuiKeA B20159027 (to JX)the Natural Science Foundation of Guangxi Zhuang Autonomous Region,No.2022GXNSFBA035545 (to YG)。
文摘Immune changes and inflammatory responses have been identified as central events in the pathological process of spinal co rd injury.They can greatly affect nerve regeneration and functional recovery.However,there is still limited understanding of the peripheral immune inflammato ry response in spinal cord inju ry.In this study.we obtained microRNA expression profiles from the peripheral blood of patients with spinal co rd injury using high-throughput sequencing.We also obtained the mRNA expression profile of spinal cord injury patients from the Gene Expression Omnibus(GEO)database(GSE151371).We identified 54 differentially expressed microRNAs and 1656 diffe rentially expressed genes using bioinformatics approaches.Functional enrichment analysis revealed that various common immune and inflammation-related signaling pathways,such as neutrophil extracellular trap formation pathway,T cell receptor signaling pathway,and nuclear factor-κB signal pathway,we re abnormally activated or inhibited in spinal cord inju ry patient samples.We applied an integrated strategy that combines weighted gene co-expression network analysis,LASSO logistic regression,and SVM-RFE algorithm and identified three biomarke rs associated with spinal cord injury:ANO10,BST1,and ZFP36L2.We verified the expression levels and diagnostic perfo rmance of these three genes in the original training dataset and clinical samples through the receiver operating characteristic curve.Quantitative polymerase chain reaction results showed that ANO20 and BST1 mRNA levels were increased and ZFP36L2 mRNA was decreased in the peripheral blood of spinal cord injury patients.We also constructed a small RNA-mRNA interaction network using Cytoscape.Additionally,we evaluated the proportion of 22 types of immune cells in the peripheral blood of spinal co rd injury patients using the CIBERSORT tool.The proportions of naive B cells,plasma cells,monocytes,and neutrophils were increased while the proportions of memory B cells,CD8^(+)T cells,resting natural killer cells,resting dendritic cells,and eosinophils were markedly decreased in spinal cord injury patients increased compared with healthy subjects,and ANO10,BST1 and ZFP26L2we re closely related to the proportion of certain immune cell types.The findings from this study provide new directions for the development of treatment strategies related to immune inflammation in spinal co rd inju ry and suggest that ANO10,BST2,and ZFP36L2 are potential biomarkers for spinal cord injury.The study was registe red in the Chinese Clinical Trial Registry(registration No.ChiCTR2200066985,December 12,2022).
基金supported by the National Key Basic Research Program of China (973 Program)under Grant No. 2009CB320401the National Natural Science Foundation of China under Grants No. 61171099, No. 61101117+1 种基金the National Key Scientific and Technological Project of China under Grants No. 2012ZX03004005-002, No. 2012ZX03003-007the Fundamental Research Funds for the Central Universities under Grant No. BUPT2012RC0112
文摘This paper investigates the uplink throughput of Cognitive Radio Cellular Networks(CRCNs).As oppose to traditional performance evaluation schemes which mainly adopt complex system level simulations,we use the theoretical framework of stochastic geometry to provide a tractable and accurate analysis of the uplink throughput in the CRCN.By modelling the positions of User Equipments(UEs)and Base Stations(BSs)as Poisson Point Processes(PPPs),we analyse and derive expressions for the link rate and the cell throughput in the Primary(PR)and Secondary(SR)networks.The expressions show that the throughput of the CRCN is mainly affected by the density ratios between the UEs and the BSs in both the PR and SR networks.Besides,a comparative analysis of the link rate between random and regular BS deployments is concluded,and the results confirm the accuracy of our analysis.Furthermore,we define the cognitive throughput gain and derive an expression which is dominated by the traffic load in the PR network.
基金Supported by Yunnan Province High-Level Health Technical Talents,Leading Talents,No.L-2019011.
文摘BACKGROUND Non-suicidal self-injury(NSSI)is common among adolescents and frequently cooccurs with depression.Understanding the distinct patterns of NSSI behaviors,along with their associated risk and protective factors,is crucial for developing effective interventions.AIM To classify NSSI behaviors and examine interactions between risk and resilience factors in Chinese adolescents.METHODS A cross-sectional study involving 3967 Chinese students(51.7%female,mean age 13.58±2.24 years)who completed questionnaires on parenting styles,bullying,childhood maltreatment,depression,resilience,and NSSI.Latent profile analysis(LPA)was used to identify NSSI subtypes,and network analysis explored interactions between risk and resilience factors.RESULTS Three NSSI subtypes were identified:NSSI with depression(18.8%),NSSI without depression(12.3%),and neither(68.9%).Bullying was the central risk factor across subtypes,while emotional control and family support were key protective factors.Statistical analyses showed significant differences between groups(P<0.001).CONCLUSION This study identified three NSSI subtypes among Chinese adolescents.Bullying emerged as a central risk factor,while emotional control and family support were key protective factors.Targeting these areas may help reduce NSSI behaviors in this population.
文摘Content analysis of scientific papers emanating from Antarctic science research during the 25 years period （1980-- 2004） has been carried out using neural network based algorithm-CATPAC. A total of 10 942 research articles published in Science Citation Indexed （SCI） journals were used for the study. Normalized co-word matrix from 35 most-used significant words was used to study the semantic association between the words. Structural Equivalence blocks were constructed from these 35 most-used words. Four-block model solution was found to be optimum. The density table was dichotomized using the mean density of the table to derive the binary matrix, which was used to construct the network map. Network maps represent the thematic character of the blocks. The blocks showed preferred connection in establishing semantic relationship with the blocks, characterizing thematic composition of Antarctic science research. The analysis has provided an analytical framework for carrying out studies on the con- tent of scientific articles. The paper has shown the utility of co-word analysis in highlighting the important areas of research in Antarctic science.
基金Supported by the National Natural Science Foundation of China under Grant Nos 61501217,61363015,61501218 and 61262020the Natural Science Foundation of Jiangxi Province under Grant No 20142BAB206026
文摘Fractal and self similarity of complex networks have attracted much attention in recent years. The fractal dimension is a useful method to describe the fractal property of networks. However, the fractal features of mobile social networks （MSNs） are inadequately investigated. In this work, a box-covering method based on the ratio of excluded mass to closeness centrality is presented to investigate the fractal feature of MSNs. Using this method, we find that some MSNs are fractal at different time intervals. Our simulation results indicate that the proposed method is available for analyzing the fractal property of MSNs.
文摘An array composed of sixteen gas sensors was constructed to analyze gas mixtures quantitatively. The data of responses from the sensor array to ethane, propane and propylene were treated by three-layer ANN with BP algorithms and PLS. The analytical results indicated that the concentration predicted with ANN is better than that with PLS. The average prediction errors for ethane, propane and propylene were 5.11%, 8.28%, 2.64%, respectively.
基金supported by the National Key Research and Development Plan Project(grant number:2022YFC3600904)The funding organization had no role in the survey’s design,implementation,and analysis.
文摘Objective:Network analysis was used to explore the complex inter-relationships between social participation activities and depressive symptoms among the Chinese older population,and the differences in network structures among different genders,age groups,and urban-rural residency would be compared.Methods:Based on the 2018 wave of the Chinese Longitudinal Healthy Longevity Survey(CLHLS),12,043 people aged 65 to 105 were included.The 10-item Center for Epidemiologic Studies Depression(CESD)Scale was used to assess depressive symptoms and 10 types of social participation activities were collected,including housework,tai-chi,square dancing,visiting and interacting with friends,garden work,reading newspapers or books,raising domestic animals,playing cards or mahjong,watching TV or listening to radio,and organized social activities.R 4.2.1 software was used to estimate the network model and calculate strength and bridge strength.Results:21.60%(2,601/12,043)of the participants had depressive symptoms.The total social participation score was negatively associated with depressive symptoms after adjusting for sociodemographic factors.The network of social participation and depressive symptoms showed that“D9(Inability to get going)”and“S9(Watching TV and/or listening to the radio)”had the highest strength within depressive symptoms and social participation communities,respectively,and“S1(Housework)”,“S9(Watching TV and/or listening to the radio)”,and“D5(Hopelessness)”were the most prominent bridging nodes between the two communities.Most edges linking the two communities were negative.“S5(Graden work)-D5(Hopelessness)”and“S6(Reading newspapers/books)-D4(Everything was an effort)”were the top 2 strongest negative edges.Older females had significantly denser network structures than older males.Compared to older people aged 65e80,the age group 81e105 showed higher network global strength.Conclusions:This study provides novel insights into the complex relationships between social participation and depressive symptoms.Except for doing housework,other social participation activities were found to be protective for depression levels.Different nursing strategies should be taken to prevent and alleviate depressive symptoms for different genders and older people of different ages.
基金supported in part by the Slovenian Research Agency(VB,research program P1-0294)(VB,research project J5-2557)+2 种基金(VB,research project J5-4596)COST EU(VB,COST action CA21163(HiTEc)is prepared within the framework of the HSE University Basic Research Program.
文摘Purpose:We analyzed the structure of a community of authors working in the field of social network analysis(SNA)based on citation indicators:direct citation and bibliographic coupling metrics.We observed patterns at the micro,meso,and macro levels of analysis.Design/methodology/approach:We used bibliometric network analysis,including the“temporal quantities”approach proposed to study temporal networks.Using a two-mode network linking publications with authors and a one-mode network of citations between the works,we constructed and analyzed the networks of citation and bibliographic coupling among authors.We used an iterated saturation data collection approach.Findings:At the macro-level,we observed the global structural features of citations between authors,showing that 80%of authors have not more than 15 citations from other works.At the meso-level,we extracted the groups of authors citing each other and similar to each other according to their citation patterns.We have seen a division of authors in SNA into groups of social scientists and physicists,as well as into other groups of authors from different disciplines.We found some examples of brokerage between different groups that maintained the common identity of the field.At the micro-level,we extracted authors with extremely high values of received citations,who can be considered as the most prominent authors in the field.We examined the temporal properties of the most popular authors.Research limitations:The main challenge in this approach is the resolution of the author’s name(synonyms and homonyms).We faced the author disambiguation,or“multiple personalities”(Harzing,2015)problem.To remain consistent and comparable with our previously published articles,we used the same SNA data collected up to 2018.The analysis and conclusions on the activity,productivity,and visibility of the authors are relative only to the field of SNA.Practical implications:The proposed approach can be utilized for similar objectives and identifying key structures and characteristics in other disciplines.This may potentially inspire the application of network approaches in other research areas,creating more authors collaborating in the field of SNA.Originality/value:We identified and applied an innovative approach and methods to study the structure of scientific communities,which allowed us to get the findings going beyond those obtained with other methods.We used a new approach to temporal network analysis,which is an important addition to the analysis as it provides detailed information on different measures for the authors and pairs of authors over time.
基金supported by the earmarked fund for CARS,China(CARS-42)the earmarked fund for Jiangsu Agricultural Industry Technology System,China(JATS(2022)331)the Jiangsu Key Research and Development Program,China(BE2021332)。
文摘The Chinese crested duck is a unique duck breed having a bulbous feather shape on its duck head.However,the mechanisms involved in its formation and development are unclear.In the present study,RNA sequencing analysis was performed on the crested tissues of 6 Chinese crested ducks and the scalp tissues of 6 cherry valley ducks(CVs)from 2 developmental stages.This study identified 261 differentially expressed genes(DEGs),122 upregulated and 139 downregulated,in the E28 stage and 361 DEGs,154 upregulated and 207 downregulated in the D42 stage between CC and CV ducks.The subsequent results of weighted gene co-expression network analysis(WGCNA)revealed that the turquoise and cyan modules were associated with the crest trait in the D42 stage,meanwhile,the green,brown,and pink modules were associated with the crest trait in the E28 stage.Venn analysis of the DEGs and WGCNA showed that 145 and 45 genes are associated between the D42 and E28 stages,respectively.The expression of WNT16,BMP2,SLC35F2,SLC6A15,APOBEC2,ABHD6,TNNC2,MYL1,and TNNI2 were verified by real-time quantitative PCR.This study provides an approach to reveal the molecular mechanisms underlying the crested trait development.
基金Under the auspices of the Key Research Base of Humanities and Social Sciences of the Ministry of Education of China(No.22JJD790029)。
文摘Data on discrete,isolated attributes of the marine economy are often used in traditional marine economic research.However,as the focus of urban research shifts from internal static attributes to external dynamic linkages,the importance of marine economic net-work research is beginning to emerge.The construction of the marine economic network in China’s coastal areas is necessary to change the flow of land and sea resources and optimize regional marine economic development.Employing data from headquarters and branches of sea-related A-share listed enterprises to construct the marine economic network in China,we use social network analysis(SNA)to discuss the characteristics of its evolution as of 2010,2015,and 2020 and its governance.The following results were obtained.1)In terms of topological characteristics,the scale of the marine economic network in China’s coastal areas has accelerated and expan-ded,and the connections have become increasingly close;thus,this development has complex network characteristics.2)In terms of spatial structure,the intensity of the connection fluctuates and does not form stable development support;the group structure gradually becomes clear,but the overall pattern is fragmented;there are spatial differences in marine economic agglomeration radiation;the radi-ation effect of the eastern marine economic circle is obvious;and the polarization effect of northern and southern marine economic circles is significant.On this basis,we construct a framework for the governance of a marine economic network with the market,the government,and industry as the three governing bodies.By clarifying the driving factors and building objectives of marine economic network construction,this study aims to foster the high-quality development of China’s marine economy.
文摘AIM:To conduct a bibliometric analysis of studies on microphthalmos and anophthalmos(M/A),explore research hotspots,and provide information on future research interests in this field to benefit clinicians and researchers.METHODS:Totally 751 publications related to M/A from the year 2004 to 2023 were collected from the Web of Science Core Collection database.These publications consist of both original and review articles,that are composed in English.The contributions of different countries,institutions,journals,and authors were analyzed,and network analysis was conducted by using Microsoft Excel 2021,VOSviewer,and R Studio to visualize research hotspots.RESULTS:Among all publications included,the highest number of publications came from USA(218,29.03%).China followed with 99 publications(13.18%),and England with 86 publications(11.45%).The publications from the USA had the highest frequency of citations,with 16699 citations,and the highest H-index of 49.The American Journal of Medical Genetics Part A(43,5.73%)published the largest number of papers,and the University of London had the most publications(41,5.46%).The genetic and molecular mechanisms of M/A were still unclear and the clinical intervention for M/A had gained a lot of attention as an emerging area of interest.CONCLUSION:Data have been gathered on the yearly count of published materials and citations,as well as the rise in publication trends,the efficiency of regions or countries,authors,journals,and organizations,along with the high-cited publications in M/A.The recent trend of research has shifted from genetic mechanisms to different clinical phenotypes and corresponding clinical interventions,which can give direction to future research.
基金funded by the National Science Foundation of China [Grant No.42071200]Second Tibetan Plateau Scientific Expedition and Research Program [Grant No.2019QZKK0902]the Western China Youth Scholars Project under the Western Light Talent Training and Recruitment Program of the Chinese Academy of Sciences.
文摘Social Network Theory and methods have emerged as pivotal tools for dissecting intricate interdisciplinary issues in rural communities.This study aims to systematically delineate the application characteristics and trends of Social Network Analysis(SNA)in rural community research.Using a twofold approach,we integrate a traditional literature review and CiteSpace bibliometric analysis to assess the application status and evolutionary trends of SNA methods in this context.The key findings include the following:①Chinese research trends:scholars predominantly concentrate on the“three rural”issues(related to agriculture,rural areas,and small-scale farmers)and social support mechanisms for vulnerable rural populations.With policy shifts,rural revitalization,tourism,governance,social trust,and multi-dimensional poverty are poised to emerge as hot topics for the future.For further refinement,we suggest that the application of SNA in rural community research could benefit from content expansion,long-term studies,and innovative modelling techniques.②Research by international scholars has been primarily directed toward the physical and mental health of rural residents,as well as socioeconomic issues.Despite these studies covering a range of typical cases across various nations,a conspicuous lack of thorough,systematic,and prolonged efforts focused on rural community development in specific regions remains.Additionally,health issues affecting rural residents are expected to sustain long-standing and focused international academic attention.This study contributes to a more nuanced understanding of the current applications and potential future directions of SNA in rural community studies,both in China and internationally.
基金Supported by National Natural Science Foundation of China,No.72104183Shanghai Municipal Health Commission Project,No.20234Y0057+4 种基金Shanghai Sailing Program,No.20YF1444900Shanghai Hospital Association Project,No.X2022142Projects of the Committee of Shanghai Science and Technology,No.20Y11913700Guangdong Association of Clinical Trials(GACT)/Chinese Thoracic Oncology Group(CTONG)and Guangdong Provincial Key Lab of Translational Medicine in Lung Cancer,No.2017B030314120Beijing CSCO(Sisco)Clinical Oncology Research Grant,No.Y-HS202101-0205.
文摘BACKGROUND Gastrointestinal neoplasm(GN)significantly impact the global cancer burden and mortality,necessitating early detection and treatment.Understanding the evolution and current state of research in this field is vital.AIM To conducts a comprehensive bibliometric analysis of publications from 1984 to 2022 to elucidate the trends and hotspots in the GN risk assessment research,focusing on key contributors,institutions,and thematic evolution.METHODS This study conducted a bibliometric analysis of data from the Web of Science Core Collection database using the"bibliometrix"R package,VOSviewer,and CiteSpace.The analysis focused on the distribution of publications,contributions by institutions and countries,and trends in keywords.The methods included data synthesis,network analysis,and visualization of international collaboration networks.RESULTS This analysis of 1371 articles on GN risk assessment revealed a notable evolution in terms of research focus and collaboration.It highlights the United States'critical role in advancing this field,with significant contributions from institutions such as Brigham and Women's Hospital and the National Cancer Institute.The last five years,substantial advancements have been made,representing nearly 45%of the examined literature.Publication rates have dramatically increased,from 20 articles in 2002 to 112 in 2022,reflecting intensified research efforts.This study underscores a growing trend toward interdisciplinary and international collaboration,with the Journal of Clinical Oncology standing out as a key publication outlet.This shift toward more comprehensive and collaborative research methods marks a significant step in addressing GN risks.CONCLUSION This study underscores advancements in GN risk assessment through genetic analyses and machine learning and reveals significant geographical disparities in research emphasis.This calls for enhanced global collaboration and integration of artificial intelligence to improve cancer prevention and treatment accuracy,ultimately enhancing worldwide patient care.
文摘Chinese Medicine(CM)has been widely used as an important avenue for disease prevention and treatment in China especially in the form of CM prescriptions combining sets of herbs to address patients’symptoms and syndromes.However,the selection and compatibility of herbs are complex and abstract due to intrinsic relationships between herbal properties and their overall functions.Network analysis is applied to demonstrate the complex relationships between individual herbal efficacy and the overall function of CM prescriptions.To illustrate their connections and correlations,prescription function(PF),prescription herb(PH),and herbal efficacy(HE)intranetworks are proposed based on CM theory to identify relationships between herbs and prescriptions.These three networks are then connected by PF-PH and PH-HE interlayer networks adopting herb dosage to form a multidimensional heterogeneous network,a Prescription-Herb-Function Network(PHFN).The network is applied to 112 classic prescriptions from Treatise on Exogenous Febrile and Miscellaneous Diseases to illustrate the application of PHFN.The PHFN is constructed including 146 functions in PF intra network,89 herbs in the PH intra network,and 163 herbal efficacies in the HE intra network.The results show that herb pairs with synergistic actions have stronger relevance,such as licorice-cassia twig,licorice-Chinese date,fresh ginger-Chinese date,etc.The integration of dosage to the network helps to indicate the main herbs for cluster analysis and automatic formulation.PHFN also reveals the internal relationships between the functions of prescriptions and composed herbal efficacies.
基金Supported by National Nature Science Foundation of China,No.82100195China Postdoctoral Science Foundation,No.2021M700777Medical Research Project of Foshan Municipal Health Bureau,No.20230349.
文摘BACKGROUND Colon cancer is acknowledged as one of the most common malignancies worldwide,ranking third in United States regarding incidence and mortality.Notably,approximately 40%of colon cancer cases harbor oncogenic KRAS mutations,resulting in the continuous activation of epidermal growth factor receptor signaling.AIM To investigate the key pathogenic genes in KRAS mutant colon cancer holds considerable importance.METHODS Weighted gene co-expression network analysis,in combination with additional bioinformatics analysis,were conducted to screen the key factors driving the progression of KRAS mutant colon cancer.Meanwhile,various in vitro experiments were also conducted to explore the biological function of transglutaminase 2(TGM2).RESULTS Integrated analysis demonstrated that TGM2 acted as an independent prognostic factor for progression-free survival.Immunohistochemical analysis on tissue microarrays revealed that TGM2 was associated with an elevated probability of perineural invasion in patients with KRAS mutant colon cancer.Additionally,biological roles of the key gene TGM2 was also assessed,suggesting that the downregulation of TGM2 attenuated the proliferation,invasion,and migration of the KRAS mutant colon cancer cell line.CONCLUSION This study underscores the potential significance of TGM2 in the progression of KRAS mutant colon cancer.This insight not only offers a theoretical foundation for therapeutic approaches but also highlights the need for additional clinical trials and fundamental research to support our preliminary findings.
文摘The inter-city linkage heat data provided by Baidu Migration is employed as a characterization of inter-city linkages in order to facilitate the study of the network linkage characteristics and hierarchical structure of urban agglomeration in the Greater Bay Area through the use of social network analysis method.This is the inaugural application of big data based on location services in the study of urban agglomeration network structure,which represents a novel research perspective on this topic.The study reveals that the density of network linkages in the Greater Bay Area urban agglomeration has reached 100%,indicating a mature network-like spatial structure.This structure has given rise to three distinct communities:Shenzhen-Dongguan-Huizhou,Guangzhou-Foshan-Zhaoqing,and Zhuhai-Zhongshan-Jiangmen.Additionally,cities within the Greater Bay Area urban agglomeration play different roles,suggesting that varying development strategies may be necessary to achieve staggered development.The study demonstrates that large datasets represented by LBS can offer novel insights and methodologies for the examination of urban agglomeration network structures,contingent on the appropriate mining and processing of the data.
文摘Introduction:Osteoarthritis(OA)is still an important health problem,and understanding its pathological mechanisms is essential for its diagnosis and treatment.There is evidence that autophagy may play a role in OA progression,but the exact mechanism remains unclear.Methods:In this study,we adopted a multi-prong approach to systematically identify the key autophagy-related genes(ARGs)associated with OA.Through weighted gene coexpression network analysis,we initially identified significant gene modules associated with OA.Subsequent differential gene analysis performed on normal and OA specimens.Further analysis later using the MCC algorithm highlighted hub ARGs.These genes were then incorporated into the prediction model of OA.In addition,the expression patterns of these DEGs were verified by in vitro experiments.Results:A total of 104 differentially expressed genes(DEGs)were identified by differential gene analysis,of which 102 were up-regulated and 2 were down-regulated.These differentially expressed genes were closely related to key signaling pathways,such as PI3K-Akt signaling pathway,IL-17 signaling pathway and osteoclast differentiation.Further MCC analysis highlighted 10 hub ARGs,among which ATF3,CYCS,FOXO3,KLF6,NFKBIA and SOCS3 were particularly significant,which were then included in the prediction model of OA,which showed robust prediction ability with an area under the curve of 0.783.In vitro experiments confirmed that the expression pattern of these DEGs was consistent with our prediction.Conclusion:In summary,our comprehensive analysis not only provides new insights into the molecular basis of OA,but also suggests potential biomarkers for its diagnosis.
