At Baiyin dairy farm, the Chinese herbal additive was added into feed which was then fed to dairy cows from August to October, 2014, and the changes in milk production and quality were observed. The test showed that t...At Baiyin dairy farm, the Chinese herbal additive was added into feed which was then fed to dairy cows from August to October, 2014, and the changes in milk production and quality were observed. The test showed that the additive added into the feed had obvious milk-increasing effect, the milk production was im- proved by 12.67%-17.26%, and the milk quality was improved. The additive has the effects of preventing miscarriage, expelling parasite and preventing diseases. The nutritional components in the feed additive were determined, and the results showed that the contents of protein, crude fat, Ca and P in the additive were 12.29%, 2.66%, 1.8% and 0.22%, respectively.展开更多
AIM: To investigate changes over time in, and effects of sealing technology on, milk test results following pancreatectomy. METHODS: From April 2008 to October 2013, 66 pancreatic resections were performed at the Iwak...AIM: To investigate changes over time in, and effects of sealing technology on, milk test results following pancreatectomy. METHODS: From April 2008 to October 2013, 66 pancreatic resections were performed at the Iwakuni Clinical Center. The milk test has been routinely conducted at the institute whenever possible during pancreatectomy. The milk test comprises the following procedure: A nasogastric tube is inserted until the third portion of the duodenum, followed by injection of 100 mL of milk through the tube. If a chyle leak is present, the patient tests positive in this milk test based on the observation of a white milky discharge. Positive milk test rates, leakage sites, and chylous ascites incidence were examined. Liga Sure?(LS; Covidien, Dublin, Ireland), a vessel-sealing device, is routinely used in pancreatectomy. Positive milk test rates before and after use of LS, as well as drain discharge volume at the 2^(nd) and 3^(rd) postoperative days, were compared retrospectively. Finally, positive milk test rates and chylous ascites incidence were compared with the results of a previous report.RESULTS: Fifty-nine milk tests were conducted during pancreatectomy. The positive milk test rate for all pancreatectomy cases was 13.6%(8 of 59 cases). One case developed postoperative chylous ascites(2.1% among the pancreatoduedenectomy cases and 1.7% among all pancreatectomies). Positive rates by procedure were 12.8% for pancreatoduodenectomy and 22.2% for distal pancreatectomy. Positive rates by disease were 17.9% for pancreatic and 5.9% for biliary diseases. When comparing results from before and after use of LS, positive milk test rates in pancreatoduodenectomy were 13.0% before and 12.5% after, while those in distal pancreatectomy were 33.3% and 0%. Drainage volume tended to decrease when LS was used on the 3^(rd) postoperative day(volumes were 424 ± 303 mL before LS and 285 ± 185 mL after, P = 0.056). Both chylous ascites incidence and positive milk test rates decreased slightly compared with those rates from the previous study. CONCLUSION: Positive milk test rates and chylous ascites incidence decreased over time. Sealing technology may thus play an important role in preventing postoperative chylous ascites.展开更多
The aim of the study was to determine the impact of residues of detergents and disinfectants on the results of most commonly used inhibitor tests for raw milk. Microbiological test (Delvotest SP-NT) and three rapid te...The aim of the study was to determine the impact of residues of detergents and disinfectants on the results of most commonly used inhibitor tests for raw milk. Microbiological test (Delvotest SP-NT) and three rapid tests (Charm 3 MRL BL/TET2, Charm ROSA MRL BL/TET and Penzym) were used in the study. Three concentrations (recommended by the manufacturer, 10 times lower and twice higher) of 36 detergents and disinfectants in raw milk were investigated. All methods did not detect concentrations of detergents and disinfectants of alkaline and acid origin 10 times lower than recommended by the manufacturer. 39% of the investigated substances of alkaline origin were detected by Delvotest SP-NT and Penzym;Charm tests showed non-typical results only. Delvotest SP-NT did not detect substances of acid origin;Penzym detected 50% of these substances, Charm tests showed only non-typical results. Delvotest SP-NT and Penzym appeared to be more sensitive to the substances used for teat hygiene and disinfection. The scope of rapid tests (receptor or enzymatic) does not cover the detection of detergent and disinfectant re- sidues in milk. However, according to the non-typical results of the test, it is possible to suspect the presence of these substances in milk. McNemar’s and Cochran’s Q tests were used for statistical analysis of the data.展开更多
(Co) variance components and genetic parameters were estimated for milk yield of Iranian Holstein cows. A total number of 68,945 milk test-day records of first, second and third lactations of 8515 animals from 100 sir...(Co) variance components and genetic parameters were estimated for milk yield of Iranian Holstein cows. A total number of 68,945 milk test-day records of first, second and third lactations of 8515 animals from 100 sires and 7743 dams originated from 34 herds collected during 2007 to 2009 by Iranian animal breeding center were used. The ASReml computer program was used to analyze the milk test-day records using the random regression procedure. Herd test date (HTD), milking times per day (milking frequency), number of lactations, year of birth, year of calving, age of animal at calving and days in milk (DIM) considered as fixed effects and additive genetic effects and animal permanent environmental effects were considered as the random effects. Additive genetic variance, animal permanent environment variance, residual variance, phenotypic variance, heritability and repeatability were estimated during different months of lactation between 5.7 - 19.6, 15.3 - 27.1, 31.4 - 17.2, 45.8 - 64.83, 0.1 - 0.32 and 0.4 - 0.6, respectively. Genetic correlation and phenotypic correlation were also estimated between months of lactation in range of -0.35 - 0.98 and 0.03 - 0.67, respectively. Genetic correlation and phenotypic correlation both showed the same changing pattern and they decreased as the interval between months of lactation increased.展开更多
Objective:To determine the presence and levels of microbes in unexpired pasteurized milk from randomly selected supermarkets in Kingston,Jamaica.Methods:The quantitative study used a stratified random sampling techniq...Objective:To determine the presence and levels of microbes in unexpired pasteurized milk from randomly selected supermarkets in Kingston,Jamaica.Methods:The quantitative study used a stratified random sampling technique in the selection of the 20 representative milk samples from six(6) supermarkets.Microbiological tests such as methylene blue reduction,standard plate count(SPC),coliform plate count(CPC),purity plate culture,gram staining and biochemical tests were performed to examine the microbes in purchased unexpired pasteurized milk.Results:One sample(BCr016) had a pH of 4.0.a rancid odour and curdled appearance.It decolourized within one hour during the methylene blue reduction test and was classified as class 4 milk.Seven of the samples were sterile with no microbe growth on the plate count agar and violet red bile salt agar(VRBA).The milk samples that appeared to be safe for consumption were all 10,11,12 and 13 days before expiration.The VRBA sample BCr016,had a colony count of 13 400 CFU/ mL.There was the presence of Escherichia coli in sample LCr021 which had a standard plate count of 1 580 SPC/mL and a coliform count of 500 CFU/mL.Enterobacter sp.was present in colonies from BCr016 and all the other milk samples.Conclusions:Unacceptable levels of Entembacter spp.and Escherichia coli were found in most of the samples.Effective measures to ensure safe milk for human consumption such as the phosphatase test and methylene blue reduction test should be routinely performed on each batch of milk processed by dairy plants.展开更多
AIM： To assess the role of lactase non-persistence/per- sistence in school-aged children and their milk-related symptoms. METHODS： The genotypes for the C/T-13910 variant associated with lactase non-persistence/ per...AIM： To assess the role of lactase non-persistence/per- sistence in school-aged children and their milk-related symptoms. METHODS： The genotypes for the C/T-13910 variant associated with lactase non-persistence/ persistence were determined using PCR-minisequencing in a group of 172 children with a mean age of 8.6 years （SE = 0.02, 93 boys） participating in a follow-up study for cow＇s milk allergy. The parents were asked to assess their children＇s milk consumption and abdominal symptoms. RESULTS： The presence of allergy to cow＇s milk was not associated with the C/C-13910 genotype related with a decline of lactase enzyme activity during childhood （lactase non-persistence）. The frequency of the C/C-13910 genotype （16%） was similar to published figures for the prevalence of adult-type hypolactasia in Finland. The majority of the children （90%） in this series consumed milk but 26% of their families suspected that their children had milk-related symptoms. Forty-eight percent of the children with the C/C-13910 genotype did not drink milk at all or consumed a low lactose containing diet prior to the genotyping （P〈 0.004 when compared to the other genotypes）. CONCLUSION： Analysis of the C/T-13910 polymorphism is an easy and reliable method for excluding adult-type hypolactasia in children with milk-related symptoms. Genotyping for this variant can be used to advise diets for children with a previous history of cow＇s milk allergy.展开更多
The aim of this study was to evaluate the impact of the somatic cell count (SCC) levels measured at 28-test-day intervals or pregnancy rate at the first artificial insemination of Holstein Friesian cows. All necessary...The aim of this study was to evaluate the impact of the somatic cell count (SCC) levels measured at 28-test-day intervals or pregnancy rate at the first artificial insemination of Holstein Friesian cows. All necessary information was taken from test day and farm records. Levels of SCC in milk, 30 days before, and 30 days after the first artificial insemination (FAI) date were divided into 4 categories and their interconnection with pregnancy rate (PR) at FAI was evaluated by applying logistic models. A SCC threshold of 150.000 cells/ml of milk was used to differentiate uninfected udders and infected udders of Holstein Friesian cows. A level of SCC > 150.000 cells/ml in milk, and a level of SCC < 150.000 cells/ml before and after FAI respectively were considered udders with new infections. A level of SCC > 150.000 cells/ml in milk before FAI as well as after FAI, was considered chronically infected udders or subclinical intramammary infections (IMI). Data from 792 FAIs from farm and test day records over a 4-year period (2019-2022) were evaluated. The outcome of this study revealed that the risk for low PR at the first AI was increased in cows with infected udders (OR = 1.33, CI 0.99 - 1.78), compared with uninfected udders. Increased levels of SCC after FAI had a negative effect on PR, while before FAI was not affected. To achieve a good PR, mastitis control measures must be fully adopted to a large extent around the first AI.展开更多
文摘At Baiyin dairy farm, the Chinese herbal additive was added into feed which was then fed to dairy cows from August to October, 2014, and the changes in milk production and quality were observed. The test showed that the additive added into the feed had obvious milk-increasing effect, the milk production was im- proved by 12.67%-17.26%, and the milk quality was improved. The additive has the effects of preventing miscarriage, expelling parasite and preventing diseases. The nutritional components in the feed additive were determined, and the results showed that the contents of protein, crude fat, Ca and P in the additive were 12.29%, 2.66%, 1.8% and 0.22%, respectively.
文摘AIM: To investigate changes over time in, and effects of sealing technology on, milk test results following pancreatectomy. METHODS: From April 2008 to October 2013, 66 pancreatic resections were performed at the Iwakuni Clinical Center. The milk test has been routinely conducted at the institute whenever possible during pancreatectomy. The milk test comprises the following procedure: A nasogastric tube is inserted until the third portion of the duodenum, followed by injection of 100 mL of milk through the tube. If a chyle leak is present, the patient tests positive in this milk test based on the observation of a white milky discharge. Positive milk test rates, leakage sites, and chylous ascites incidence were examined. Liga Sure?(LS; Covidien, Dublin, Ireland), a vessel-sealing device, is routinely used in pancreatectomy. Positive milk test rates before and after use of LS, as well as drain discharge volume at the 2^(nd) and 3^(rd) postoperative days, were compared retrospectively. Finally, positive milk test rates and chylous ascites incidence were compared with the results of a previous report.RESULTS: Fifty-nine milk tests were conducted during pancreatectomy. The positive milk test rate for all pancreatectomy cases was 13.6%(8 of 59 cases). One case developed postoperative chylous ascites(2.1% among the pancreatoduedenectomy cases and 1.7% among all pancreatectomies). Positive rates by procedure were 12.8% for pancreatoduodenectomy and 22.2% for distal pancreatectomy. Positive rates by disease were 17.9% for pancreatic and 5.9% for biliary diseases. When comparing results from before and after use of LS, positive milk test rates in pancreatoduodenectomy were 13.0% before and 12.5% after, while those in distal pancreatectomy were 33.3% and 0%. Drainage volume tended to decrease when LS was used on the 3^(rd) postoperative day(volumes were 424 ± 303 mL before LS and 285 ± 185 mL after, P = 0.056). Both chylous ascites incidence and positive milk test rates decreased slightly compared with those rates from the previous study. CONCLUSION: Positive milk test rates and chylous ascites incidence decreased over time. Sealing technology may thus play an important role in preventing postoperative chylous ascites.
基金supported by the Lithuanian Ministry of Agriculture.
文摘The aim of the study was to determine the impact of residues of detergents and disinfectants on the results of most commonly used inhibitor tests for raw milk. Microbiological test (Delvotest SP-NT) and three rapid tests (Charm 3 MRL BL/TET2, Charm ROSA MRL BL/TET and Penzym) were used in the study. Three concentrations (recommended by the manufacturer, 10 times lower and twice higher) of 36 detergents and disinfectants in raw milk were investigated. All methods did not detect concentrations of detergents and disinfectants of alkaline and acid origin 10 times lower than recommended by the manufacturer. 39% of the investigated substances of alkaline origin were detected by Delvotest SP-NT and Penzym;Charm tests showed non-typical results only. Delvotest SP-NT did not detect substances of acid origin;Penzym detected 50% of these substances, Charm tests showed only non-typical results. Delvotest SP-NT and Penzym appeared to be more sensitive to the substances used for teat hygiene and disinfection. The scope of rapid tests (receptor or enzymatic) does not cover the detection of detergent and disinfectant re- sidues in milk. However, according to the non-typical results of the test, it is possible to suspect the presence of these substances in milk. McNemar’s and Cochran’s Q tests were used for statistical analysis of the data.
文摘(Co) variance components and genetic parameters were estimated for milk yield of Iranian Holstein cows. A total number of 68,945 milk test-day records of first, second and third lactations of 8515 animals from 100 sires and 7743 dams originated from 34 herds collected during 2007 to 2009 by Iranian animal breeding center were used. The ASReml computer program was used to analyze the milk test-day records using the random regression procedure. Herd test date (HTD), milking times per day (milking frequency), number of lactations, year of birth, year of calving, age of animal at calving and days in milk (DIM) considered as fixed effects and additive genetic effects and animal permanent environmental effects were considered as the random effects. Additive genetic variance, animal permanent environment variance, residual variance, phenotypic variance, heritability and repeatability were estimated during different months of lactation between 5.7 - 19.6, 15.3 - 27.1, 31.4 - 17.2, 45.8 - 64.83, 0.1 - 0.32 and 0.4 - 0.6, respectively. Genetic correlation and phenotypic correlation were also estimated between months of lactation in range of -0.35 - 0.98 and 0.03 - 0.67, respectively. Genetic correlation and phenotypic correlation both showed the same changing pattern and they decreased as the interval between months of lactation increased.
文摘Objective:To determine the presence and levels of microbes in unexpired pasteurized milk from randomly selected supermarkets in Kingston,Jamaica.Methods:The quantitative study used a stratified random sampling technique in the selection of the 20 representative milk samples from six(6) supermarkets.Microbiological tests such as methylene blue reduction,standard plate count(SPC),coliform plate count(CPC),purity plate culture,gram staining and biochemical tests were performed to examine the microbes in purchased unexpired pasteurized milk.Results:One sample(BCr016) had a pH of 4.0.a rancid odour and curdled appearance.It decolourized within one hour during the methylene blue reduction test and was classified as class 4 milk.Seven of the samples were sterile with no microbe growth on the plate count agar and violet red bile salt agar(VRBA).The milk samples that appeared to be safe for consumption were all 10,11,12 and 13 days before expiration.The VRBA sample BCr016,had a colony count of 13 400 CFU/ mL.There was the presence of Escherichia coli in sample LCr021 which had a standard plate count of 1 580 SPC/mL and a coliform count of 500 CFU/mL.Enterobacter sp.was present in colonies from BCr016 and all the other milk samples.Conclusions:Unacceptable levels of Entembacter spp.and Escherichia coli were found in most of the samples.Effective measures to ensure safe milk for human consumption such as the phosphatase test and methylene blue reduction test should be routinely performed on each batch of milk processed by dairy plants.
基金Supported by The Sigrid Juselius Foundation, Helsinki, Finland The Helsinki University Hospital Research Funding, Helsinki, Finland The Helsinki University Science Foundation, Helsinki, Finland and The Foundation of Nutrition Research, Helsinki, Finland
文摘AIM： To assess the role of lactase non-persistence/per- sistence in school-aged children and their milk-related symptoms. METHODS： The genotypes for the C/T-13910 variant associated with lactase non-persistence/ persistence were determined using PCR-minisequencing in a group of 172 children with a mean age of 8.6 years （SE = 0.02, 93 boys） participating in a follow-up study for cow＇s milk allergy. The parents were asked to assess their children＇s milk consumption and abdominal symptoms. RESULTS： The presence of allergy to cow＇s milk was not associated with the C/C-13910 genotype related with a decline of lactase enzyme activity during childhood （lactase non-persistence）. The frequency of the C/C-13910 genotype （16%） was similar to published figures for the prevalence of adult-type hypolactasia in Finland. The majority of the children （90%） in this series consumed milk but 26% of their families suspected that their children had milk-related symptoms. Forty-eight percent of the children with the C/C-13910 genotype did not drink milk at all or consumed a low lactose containing diet prior to the genotyping （P〈 0.004 when compared to the other genotypes）. CONCLUSION： Analysis of the C/T-13910 polymorphism is an easy and reliable method for excluding adult-type hypolactasia in children with milk-related symptoms. Genotyping for this variant can be used to advise diets for children with a previous history of cow＇s milk allergy.
文摘The aim of this study was to evaluate the impact of the somatic cell count (SCC) levels measured at 28-test-day intervals or pregnancy rate at the first artificial insemination of Holstein Friesian cows. All necessary information was taken from test day and farm records. Levels of SCC in milk, 30 days before, and 30 days after the first artificial insemination (FAI) date were divided into 4 categories and their interconnection with pregnancy rate (PR) at FAI was evaluated by applying logistic models. A SCC threshold of 150.000 cells/ml of milk was used to differentiate uninfected udders and infected udders of Holstein Friesian cows. A level of SCC > 150.000 cells/ml in milk, and a level of SCC < 150.000 cells/ml before and after FAI respectively were considered udders with new infections. A level of SCC > 150.000 cells/ml in milk before FAI as well as after FAI, was considered chronically infected udders or subclinical intramammary infections (IMI). Data from 792 FAIs from farm and test day records over a 4-year period (2019-2022) were evaluated. The outcome of this study revealed that the risk for low PR at the first AI was increased in cows with infected udders (OR = 1.33, CI 0.99 - 1.78), compared with uninfected udders. Increased levels of SCC after FAI had a negative effect on PR, while before FAI was not affected. To achieve a good PR, mastitis control measures must be fully adopted to a large extent around the first AI.
