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Real-time crash prediction on freeways using data mining and emerging techniques 被引量:5
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作者 Jinming You Junhua Wang Jingqiu Guo 《Journal of Modern Transportation》 2017年第2期116-123,共8页
Recent advances in intelligent transportation system allow traffic safety studies to extend from historic data-based analyses to real-time applications. The study presents a new method to predict crash likelihood with... Recent advances in intelligent transportation system allow traffic safety studies to extend from historic data-based analyses to real-time applications. The study presents a new method to predict crash likelihood with traffic data collected by discrete loop detectors as well as the web-crawl weather data. Matched case-control method and support vector machines (SVMs) technique were employed to identify the risk status. The adaptive synthetic over-sampling technique was applied to solve the imbalanced dataset issues. Random forest technique was applied to select the contributing factors and avoid the over-fitting issues. The results indicate that the SVMs classifier could successfully classify 76.32% of the crashes on the test dataset and 87.52% of the crashes on the overall dataset, which were relatively satisfactory compared with the results of the previous studies. Compared with the SVMs classifier without the data, the SVMs classifier with the web-crawl weather data increased the crash prediction accuracy by 1.32% and decreased the false alarm rate by 1.72%, showing the potential value of the massive web weather data. Mean impact value method was employed to evaluate the variable effects, and the results are identical with the results of most of previous studies. The emerging technique based on the discrete traffic data and web weather data proves to be more applicable on real- time safety management on freeways. 展开更多
关键词 Crash prediction detectors Web-crawl data real time - Discrete loop Support vector machines
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人5-羟色胺2c受体基因实时荧光定量PCR方法的建立 被引量:1
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作者 刀筱芳 何进宇 +5 位作者 伍学英 龚玉来 高利民 徐亚欧 李宁 袁忠 《四川医学》 CAS 2008年第5期503-505,共3页
目的建立5-羟色胺2c受体(5-HTR2c)基因mRNA表达水平的TaqMan real—time PcR检测方法。方法以β-actin为内参基因,根据GenBank中人5-HTR2c及β-actin基因序列,分别设计了2套特异性引物和TaqMan探针,接着对反应的退火温度、引物浓... 目的建立5-羟色胺2c受体(5-HTR2c)基因mRNA表达水平的TaqMan real—time PcR检测方法。方法以β-actin为内参基因,根据GenBank中人5-HTR2c及β-actin基因序列,分别设计了2套特异性引物和TaqMan探针,接着对反应的退火温度、引物浓度、探针浓度、Mg^2+浓度进行优化,然后以优化的条件建立相对定量标准曲线,并对该方法的稳定性进行了分析。结果5-HTR2c及β-actin基因的real—time PCR扩增效率分别为99.9%和100.0%;相对定量标准曲线的CT值线性范围分别为12.2~35.1和11.5~31.5,相关系数分别为0.999及1.000;批内及批问变异系数(8.0%。结论本研究所建立的针对5-HTR2c mRNA表达水平的Taqman real-time PCR检测方法具有扩增效率高、稳定性好等特点,为进一步探索5-HTR2c的功能及其mRNA表达水平的变化和各种疾病发生、发展的相关性提供了有用的方法学基础。 展开更多
关键词 5-羟色胺2c受体 TAQMAN探针 realtime PCR
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小麦醇溶-谷蛋白盒结合因子基因表达特性的研究 被引量:3
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作者 牛静勇 陈占宽 贺浩华 《麦类作物学报》 CAS CSCD 北大核心 2009年第6期941-946,共6页
为了给小麦品质改良的基因工程研究提供参考依据,以小麦醇溶-谷蛋白盒结合因子(Wheat Prola-min-Box Binding Factor,WPBF)基因为研究对象,从定性和定量两个方面,利用RT-PCR与Real Time PCR系统地研究了WPBF基因在小麦不同组织器官中以... 为了给小麦品质改良的基因工程研究提供参考依据,以小麦醇溶-谷蛋白盒结合因子(Wheat Prola-min-Box Binding Factor,WPBF)基因为研究对象,从定性和定量两个方面,利用RT-PCR与Real Time PCR系统地研究了WPBF基因在小麦不同组织器官中以及在小麦胚乳整个发育过程中表达的时空特异性。结果表明,WPBF基因的表达是胚乳特异性的,表达跨胚乳的整个发育过程,在其表达的高峰阶段,表达强度为参照基因β-Actin的43%。 展开更多
关键词 小麦 醇溶-谷蛋白盒结合因子 RT-PCR real time PCR
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气象观测场在汽车试验场中的应用研究
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作者 陈海建 《时代汽车》 2024年第14期172-174,178,共4页
汽车试验场作为汽车开展道路测试的重要场所,用于验证汽车产品的品质以及可靠性。除了场地道路外,气象条件作为汽车道路测试的重要一环,在《GB/T12534-1990汽车道路试验方法通则》中也有明确要求,如:试验时应是无雨无雾天气,相对湿度小... 汽车试验场作为汽车开展道路测试的重要场所,用于验证汽车产品的品质以及可靠性。除了场地道路外,气象条件作为汽车道路测试的重要一环,在《GB/T12534-1990汽车道路试验方法通则》中也有明确要求,如:试验时应是无雨无雾天气,相对湿度小于95%,气温0-40℃,风速不大于3m/s。同时气象条件也作为试验场道路管控的重要依据,实时风速、雨量、能见度等信息为场地管理者发布限速、限行、封场等通知提供必要参考依据,直接影响道路测试安全管控的及时性。因此,文章从气象观测场的建设、气象服务、异常天气道路管控等方面开展气象观测场在汽车试验场中的应用研究。 展开更多
关键词 汽车试验场 气象服务 道路管控 products. In addition to the SITE roads METEOROLOGICAL conditions are an important part of AUTOMOTIVE ROAD testing and there are also clear requirements in the GB/T12534-1990 General Rules for AUTOMOTIVE ROAD Test Methods. For example the test should be conducted in rain and fog free weather with a relative humidity of less than 95% a temperature of 0-40 and a wind SPEED of no more than 3m/s. At the same time METEOROLOGICAL conditions also serve as an important basis for ROAD control in the test site. real time information such as wind SPEED rainfall and visibility provides necessary reference for SITE managers to issue notices on SPEED limits SITE closures and traffic restrictions directly affecting the timeliness of ROAD testing safety control. Therefore this article conducts research on the application of METEOROLOGICAL observation SITES in AUTOMOTIVE testing SITES from the construction of METEOROLOGICAL observation SITES METEOROLOGICAL services and abnormal weather ROAD control.
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