目的:探究维生素A辅助治疗支气管哮喘患儿的临床效果及其对淋巴细胞亚群、免疫球蛋白、白细胞介素-5(interleukin-5,IL-5)的影响。方法:支气管哮喘患儿120例,随机分为观察组和对照组各60例。两组患儿均接受哮喘正规治疗,观察组同时予以...目的:探究维生素A辅助治疗支气管哮喘患儿的临床效果及其对淋巴细胞亚群、免疫球蛋白、白细胞介素-5(interleukin-5,IL-5)的影响。方法:支气管哮喘患儿120例,随机分为观察组和对照组各60例。两组患儿均接受哮喘正规治疗,观察组同时予以维生素A补充治疗。比较两组血清维生素A水平、症状消失时间、儿童哮喘控制测试(childhood asthma control test,C-ACT)评分、肺功能[1秒用力呼气容积(forced expiratory volume in one second,FEV1)、用力肺活量(forced vital capacity,FVC)、最大呼气流量(peak expiratory flow,PEF)及FEV1/FVC]、辅助性T细胞(helper T cells,CD4+)、细胞毒性T细胞(cytotoxic T cells,CD8+)水平及辅助性T细胞/细胞毒性T细胞的比值(CD4+/CD8+)、血清免疫球蛋白A(immunoglobulin A,IgA)、免疫球蛋白G(immunoglobulin G,IgG)、免疫球蛋白E(immunoglobulin E,IgE)水平以及IL-5水平。结果:治疗后观察组维生素A水平正常患儿占比为80.00%,高于对照组的48.33%(P<0.05)。观察组患儿喘息、咳嗽、肺部啰音等症状消失时间早于对照组(均P<0.001)。治疗后观察组FEV1、FVC、PEF及FEV1/FVC分别为(2.04±0.57)L、(3.14±0.85)L、(3.98±1.14)L/s和(61.51±6.31)%,高于对照组的(1.76±0.34)L、(2.67±0.71)L、(3.41±1.02)L/s和(56.28±5.76)%(均P<0.001)。治疗后观察组C-ACT评分(24.35±2.87)分,高于对照组的(22.73±2.52)分(P<0.001)。治疗后观察组CD4+T细胞水平和CD4+/CD8+比值分别为(41.71±4.29)%和(1.85±0.44),高于对照组的(37.44±3.42)%和(1.23±0.37),观察组CD8+T细胞(24.42±2.19)%,低于对照组的(28.03±3.07)%(均P<0.001)。治疗后观察组IgA、IgG水平分别为(1.29±0.43)g/L和(12.38±2.47)g/L,高于对照组的(0.88±0.25)g/L和(10.96±1.83)g/L,治疗后观察组IgE为(264.37±26.84)U/mL,低于对照组的(324.67±33.19)U/mL(均P<0.001)。治疗后观察组IL-5水平为(70.92±7.83)μg/L,低于对照组的(81.93±8.57)μg/L(P<0.05)。结论:维生素A辅助治疗可有效提高支气管哮喘患儿维生素A水平,改善临床症状、肺功能、淋巴细胞亚群及免疫球蛋白水平、IL-5水平,值得临床推广应用。展开更多
Summary: In order to explore the role of activator protein-1 (AP-1) in the transcription of interleukin-5 (IL-5) gene regulated by protein kinase C (PKC) signal in peripheral blood T lymphocytes from asthmatic patient...Summary: In order to explore the role of activator protein-1 (AP-1) in the transcription of interleukin-5 (IL-5) gene regulated by protein kinase C (PKC) signal in peripheral blood T lymphocytes from asthmatic patient, T lymphocytes were isolated and purified from peripheral blood of each asthmatic patient. The T lymphocytes were randomly divided into 4 groups: group A (blank control), group B (treated with PKC agonist phorbol 12-myristate 13-acetate (PMA)), Group C (treated with PMA and AP-1 cis-element decoy oligodeoxynucleotides (decoy ODNs)), and group D (treated with PMA and AP-1 mutant decoy ODNs). The ODNs were transfected into the T cells of group C and D by cation liposome respectively. Reverse transcription-polymerase chain reaction (RT-PCR) was employed to assess IL-5 mRNA expression, and electrophoretic mobility shift assays (EMSA) for the activation of AP-1. The results showed that the activation of AP-1 (88 003.58±1 626.57) and the expression of IL-5 mRNA (0.8300±0.0294) in T lymphocytes stimulated with PMA were significantly higher than these in blank control (20 888.47±1103.56 and 0.3050±0.0208, respectively, P< 0.01), while the indexes (23 219.83±1 024.86 and 0.3425±0.0171 respectively) of T lymphocytes stimulated with PMA and AP-1 decoy ODNs were significantly inhibited, as compared with group B (P< 0.01). The indexes (87 107.41±1 342.92 and 0.8225±0.0222, respectively) in T lymphocytes stimulated with PMA and AP-1 mutant decoy ODNs did not exhibit significant changes, as compared with group B (P>0.05). The significant positive correlation was found between the activation of AP-1 and the expression of IL-5 mRNA (P< 0.01). It was concluded that AP-1 might participate in the signal transduction of PKC-triggered transcription of IL-5 gene in asthmatic T lymphocytes. This suggests the activation of PKC/AP-1 signal transduction cascade of T lymphocytes may play an important role in the pathogenesis of asthma.展开更多
AIM To study the role of interleukin 1β converting enzyme (ICE) in antitumor drug induced apoptosis in tumor cells. METHODS Morphological changes in human esophageal carcinoma Eca 109 cells after treated with 5 ...AIM To study the role of interleukin 1β converting enzyme (ICE) in antitumor drug induced apoptosis in tumor cells. METHODS Morphological changes in human esophageal carcinoma Eca 109 cells after treated with 5 fluorouracil (5 FU) were observed under light and electron microscope. Expression of ICE in the tumor cells exposed to 5 FU was examined by the immunocytochemical method. RESULTS The cells treated with 5 FU displayed disappearance of nucleoli, chromatin gathering under nuclear envelope, karyorrhexis, budding and the formation of apoptotic bodies. The expression of ICE was negative in control cells, and 5 FU could induce the ICE expression in Eca 109 cells undergoing apoptosis. The number and the staining intensity of positive cells increased with the extension of action time. CONCLUSION 5 FU may induce apoptosis in human esophageal carcinoma Eca 109 cells; ICE gene may be involved in the regulation of 5 FU induced apoptosis; and ICE protein may mediate apoptosis induced by 5 FU.展开更多
文摘目的:探究维生素A辅助治疗支气管哮喘患儿的临床效果及其对淋巴细胞亚群、免疫球蛋白、白细胞介素-5(interleukin-5,IL-5)的影响。方法:支气管哮喘患儿120例,随机分为观察组和对照组各60例。两组患儿均接受哮喘正规治疗,观察组同时予以维生素A补充治疗。比较两组血清维生素A水平、症状消失时间、儿童哮喘控制测试(childhood asthma control test,C-ACT)评分、肺功能[1秒用力呼气容积(forced expiratory volume in one second,FEV1)、用力肺活量(forced vital capacity,FVC)、最大呼气流量(peak expiratory flow,PEF)及FEV1/FVC]、辅助性T细胞(helper T cells,CD4+)、细胞毒性T细胞(cytotoxic T cells,CD8+)水平及辅助性T细胞/细胞毒性T细胞的比值(CD4+/CD8+)、血清免疫球蛋白A(immunoglobulin A,IgA)、免疫球蛋白G(immunoglobulin G,IgG)、免疫球蛋白E(immunoglobulin E,IgE)水平以及IL-5水平。结果:治疗后观察组维生素A水平正常患儿占比为80.00%,高于对照组的48.33%(P<0.05)。观察组患儿喘息、咳嗽、肺部啰音等症状消失时间早于对照组(均P<0.001)。治疗后观察组FEV1、FVC、PEF及FEV1/FVC分别为(2.04±0.57)L、(3.14±0.85)L、(3.98±1.14)L/s和(61.51±6.31)%,高于对照组的(1.76±0.34)L、(2.67±0.71)L、(3.41±1.02)L/s和(56.28±5.76)%(均P<0.001)。治疗后观察组C-ACT评分(24.35±2.87)分,高于对照组的(22.73±2.52)分(P<0.001)。治疗后观察组CD4+T细胞水平和CD4+/CD8+比值分别为(41.71±4.29)%和(1.85±0.44),高于对照组的(37.44±3.42)%和(1.23±0.37),观察组CD8+T细胞(24.42±2.19)%,低于对照组的(28.03±3.07)%(均P<0.001)。治疗后观察组IgA、IgG水平分别为(1.29±0.43)g/L和(12.38±2.47)g/L,高于对照组的(0.88±0.25)g/L和(10.96±1.83)g/L,治疗后观察组IgE为(264.37±26.84)U/mL,低于对照组的(324.67±33.19)U/mL(均P<0.001)。治疗后观察组IL-5水平为(70.92±7.83)μg/L,低于对照组的(81.93±8.57)μg/L(P<0.05)。结论:维生素A辅助治疗可有效提高支气管哮喘患儿维生素A水平,改善临床症状、肺功能、淋巴细胞亚群及免疫球蛋白水平、IL-5水平,值得临床推广应用。
文摘Summary: In order to explore the role of activator protein-1 (AP-1) in the transcription of interleukin-5 (IL-5) gene regulated by protein kinase C (PKC) signal in peripheral blood T lymphocytes from asthmatic patient, T lymphocytes were isolated and purified from peripheral blood of each asthmatic patient. The T lymphocytes were randomly divided into 4 groups: group A (blank control), group B (treated with PKC agonist phorbol 12-myristate 13-acetate (PMA)), Group C (treated with PMA and AP-1 cis-element decoy oligodeoxynucleotides (decoy ODNs)), and group D (treated with PMA and AP-1 mutant decoy ODNs). The ODNs were transfected into the T cells of group C and D by cation liposome respectively. Reverse transcription-polymerase chain reaction (RT-PCR) was employed to assess IL-5 mRNA expression, and electrophoretic mobility shift assays (EMSA) for the activation of AP-1. The results showed that the activation of AP-1 (88 003.58±1 626.57) and the expression of IL-5 mRNA (0.8300±0.0294) in T lymphocytes stimulated with PMA were significantly higher than these in blank control (20 888.47±1103.56 and 0.3050±0.0208, respectively, P< 0.01), while the indexes (23 219.83±1 024.86 and 0.3425±0.0171 respectively) of T lymphocytes stimulated with PMA and AP-1 decoy ODNs were significantly inhibited, as compared with group B (P< 0.01). The indexes (87 107.41±1 342.92 and 0.8225±0.0222, respectively) in T lymphocytes stimulated with PMA and AP-1 mutant decoy ODNs did not exhibit significant changes, as compared with group B (P>0.05). The significant positive correlation was found between the activation of AP-1 and the expression of IL-5 mRNA (P< 0.01). It was concluded that AP-1 might participate in the signal transduction of PKC-triggered transcription of IL-5 gene in asthmatic T lymphocytes. This suggests the activation of PKC/AP-1 signal transduction cascade of T lymphocytes may play an important role in the pathogenesis of asthma.
文摘AIM To study the role of interleukin 1β converting enzyme (ICE) in antitumor drug induced apoptosis in tumor cells. METHODS Morphological changes in human esophageal carcinoma Eca 109 cells after treated with 5 fluorouracil (5 FU) were observed under light and electron microscope. Expression of ICE in the tumor cells exposed to 5 FU was examined by the immunocytochemical method. RESULTS The cells treated with 5 FU displayed disappearance of nucleoli, chromatin gathering under nuclear envelope, karyorrhexis, budding and the formation of apoptotic bodies. The expression of ICE was negative in control cells, and 5 FU could induce the ICE expression in Eca 109 cells undergoing apoptosis. The number and the staining intensity of positive cells increased with the extension of action time. CONCLUSION 5 FU may induce apoptosis in human esophageal carcinoma Eca 109 cells; ICE gene may be involved in the regulation of 5 FU induced apoptosis; and ICE protein may mediate apoptosis induced by 5 FU.
