[Objective] The aim of this study was to study on expression of Bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay （ELISA） was used to measure Bt protein expression in different...[Objective] The aim of this study was to study on expression of Bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay （ELISA） was used to measure Bt protein expression in different tissues of transgenic pest-resistant rice at same growth stage. [Result] Absolute content of Bt protein from high to low was as follows： leaves 〉 immature seeds and glumes 〉 roots 〉 stems in different tissues of transgenic rice in grain-filling stage; Bt protein content of trans- genic rice changed a little in different growth stages （including tillering stage, booting stage, and grain-filling stage）; in general, its level declined a little in later growth stage, but the resistibility would not be influenced significantly. [Conclusion] The ex- periment is significant for pest prevention and transgenic rice breeding.展开更多
A generalized Kadomtsev–Petviashvili equation is studied by nonlocal symmetry method and consistent Riccati expansion(CRE) method in this paper. Applying the truncated Painlevé analysis to the generalized Kadomt...A generalized Kadomtsev–Petviashvili equation is studied by nonlocal symmetry method and consistent Riccati expansion(CRE) method in this paper. Applying the truncated Painlevé analysis to the generalized Kadomtsev–Petviashvili equation, some B¨acklund transformations(BTs) including auto-BT and non-auto-BT are obtained. The auto-BT leads to a nonlocal symmetry which corresponds to the residual of the truncated Painlevé expansion. Then the nonlocal symmetry is localized to the corresponding nonlocal group by introducing two new variables. Further,by applying the Lie point symmetry method to the prolonged system, a new type of finite symmetry transformation is derived. In addition, the generalized Kadomtsev–Petviashvili equation is proved consistent Riccati expansion(CRE)solvable. As a result, the soliton-cnoidal wave interaction solutions of the equation are explicitly given, which are difficult to be found by other traditional methods. Moreover, figures are given out to show the properties of the explicit analytic interaction solutions.展开更多
基金Transgenic cry1C Novel Materials of Japonica Rice Resistant Against Snout Moth’s Larva Cultivated with Biotechnology (201205068)The National Program of Transgenic Variety Development of China (2011ZX08001-001)~~
文摘[Objective] The aim of this study was to study on expression of Bt protein in transgenic pest-resistant rice. [Method] Enzyme-linked immunosorbent assay （ELISA） was used to measure Bt protein expression in different tissues of transgenic pest-resistant rice at same growth stage. [Result] Absolute content of Bt protein from high to low was as follows： leaves 〉 immature seeds and glumes 〉 roots 〉 stems in different tissues of transgenic rice in grain-filling stage; Bt protein content of trans- genic rice changed a little in different growth stages （including tillering stage, booting stage, and grain-filling stage）; in general, its level declined a little in later growth stage, but the resistibility would not be influenced significantly. [Conclusion] The ex- periment is significant for pest prevention and transgenic rice breeding.
基金Supported by the Global Change Research Program of China under Grant No.2015CB953904National Natural Science Foundation of under Grant Nos.11275072 and 11435005+3 种基金Doctoral Program of Higher Education of China under Grant No.20120076110024the Network Information Physics Calculation of Basic Research Innovation Research Group of China under Grant No.61321064Shanghai Collaborative Innovation Center of Trustworthy Software for Internet of Things under Grant No.ZF1213Zhejiang Provincial Natural Science Foundation of China under Grant No.LY14A010005
文摘A generalized Kadomtsev–Petviashvili equation is studied by nonlocal symmetry method and consistent Riccati expansion(CRE) method in this paper. Applying the truncated Painlevé analysis to the generalized Kadomtsev–Petviashvili equation, some B¨acklund transformations(BTs) including auto-BT and non-auto-BT are obtained. The auto-BT leads to a nonlocal symmetry which corresponds to the residual of the truncated Painlevé expansion. Then the nonlocal symmetry is localized to the corresponding nonlocal group by introducing two new variables. Further,by applying the Lie point symmetry method to the prolonged system, a new type of finite symmetry transformation is derived. In addition, the generalized Kadomtsev–Petviashvili equation is proved consistent Riccati expansion(CRE)solvable. As a result, the soliton-cnoidal wave interaction solutions of the equation are explicitly given, which are difficult to be found by other traditional methods. Moreover, figures are given out to show the properties of the explicit analytic interaction solutions.
