A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron micr...A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fou-rier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform si-multaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction seg-mentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm.展开更多
基金江苏省三维打印装备与制造重点实验室承担的2013年江苏省科技支撑重点项目"基于数字化微喷工艺的三维打印成型设备研制及装备集成"(3D printing forming device manufacturing and equipment integration based on digital micro jetting)(项目编号:BE2013012-2)国家自然基金"基于数字化微滴喷射工艺的异质材料零件直接制造基础研究"(项目编号:61273243)江苏省高校自然科学基金"微喷三维打印导电银浆成型基础研究"(项目编号:15KJB510018)
文摘A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fou-rier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform si-multaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction seg-mentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm.