AIM:To investigate the differences in cultivable gut bacteria and peroxisome proliferator-activated receptor γ2(PPAR-γ2) gene Pro12Ala variation in obese and normal-weight Chinese people.METHODS:Using culture method...AIM:To investigate the differences in cultivable gut bacteria and peroxisome proliferator-activated receptor γ2(PPAR-γ2) gene Pro12Ala variation in obese and normal-weight Chinese people.METHODS:Using culture methods,the amounts of Escherichia coli,Enterococci,Bacteroides,Lactobacilli,Bif idobacteria and Clostridium perfringens(C.perfringens) in the feces of 52 obese participants [body mass index(BMI):≥ 28 kg/m2] and 52 participants of normalweight(BMI:18.5-24 kg/m2) were obtained.Study participants completed comprehensive questionnaires and underwent clinical laboratory tests.The polymerase chain reaction-restriction fragment length polymorphism(PCR-PFLP) assay was used to analyze PPAR-γ2 gene Pro12Ala variation.RESULTS:The obese group exhibited a lower amount of C.perfringens(6.54 ± 0.65 vs 6.94 ± 0.57,P = 0.001)and Bacteroides(9.81 ± 0.58 vs 10.06 ± 0.39,P = 0.012) than their normal-weight counterparts.No major differences were observed in Pro12Ala genotype distribution between the two groups;however,obese individuals with a Pro/Ala genotype had a signif icantly lower level of Bacteroides(9.45 ± 0.62 vs 9.93 ± 0.51,P = 0.027) than those with a Pro/Pro genotype.In addition,the obese group demonstrated a higher stool frequency(U = 975,P < 0.001) and a looser stool(U = 1062,P = 0.015) than the normal-weight group.CONCLUSION:Our results indicated interactions among cultivable gut flora,host genetic factors and obese phenotype and this might be helpful for obesity prevention.展开更多
Predicting how human activity will influence the response of alpine grasslands to future warming has many uncertainties.In this study, a field experiment with controlled warming and clipping was conducted in an alpine...Predicting how human activity will influence the response of alpine grasslands to future warming has many uncertainties.In this study, a field experiment with controlled warming and clipping was conducted in an alpine meadow at three elevations（4313 m, 4513 m and 4693 m） in Northern Tibet to test the hypothesis that clipping would alter warming effect on biomass production.Open top chambers（OTCs） were used to increase temperature since July,2008 and the OTCs increased air temperature by approximately 0.9o C ~ 1.8o C during the growing in2012.Clipping was conducted three times one year during growing season and the aboveground parts of all live plants were clipped to approximately 0.01 m in height using scissors since 2009.Gross primary production（GPP） was calculated from the Moderate-Resolution Imaging Spectroradiometer GPP algorithm and aboveground plant production was estimated using the surface-measured normalized difference vegetation index in 2012.Warming decreased the GPP, aboveground biomass（AGB） and aboveground net primary production（ANPP） at all three elevations when clipping was not applied.In contrast, warming increased AGB at all three elevations, GPP at the two lower elevations and ANPP at the two higher elevations when clipping was applied.These findings show that clipping reduced the negative effect of warming on GPP, AGB and ANPP, suggesting that clipping may reduce the effect of climate warming on GPP, AGB and ANPP in alpine meadows on the Tibetan Plateau, and therefore, may be a viable strategy for mitigating the effects of climate change on grazing and animal husbandry on the Tibetan Plateau.展开更多
Generally the irreducible water saturation of low permeability gas reservoir is quite high which leads to the permeability stress sensibility and threshold pressure gradient. Under the assumption that permeability var...Generally the irreducible water saturation of low permeability gas reservoir is quite high which leads to the permeability stress sensibility and threshold pressure gradient. Under the assumption that permeability varies with experimental law of the pseudo pressure drop, according to concepts of perturbable ellipses and equivalent developing regulations, the calculation method of stable production of hydraulically fractured gas well in low permeability reservoirs is investigated with threshold pressure. And productivity curve is drawn and analyzed. The result shows that, permeability modulus and threshold pressure have effect on production of fractured gas well. The higher the permeability modulus and the threshold pressure, the lower the production is. Therefore, the impact of stress sensitive and threshold pressure must he considered when analyzing the productivity of vertical fracture well in low permeability gas reservoir.展开更多
Precipitation(PPT)is the primary climatic determinant of plant growth and aboveground net primary productivity(ANPP)for many of the world’s major terrestrial ecosystems.Thus,relationships between PPT and productivity...Precipitation(PPT)is the primary climatic determinant of plant growth and aboveground net primary productivity(ANPP)for many of the world’s major terrestrial ecosystems.Thus,relationships between PPT and productivity can provide insight into how changes in climate may alter ecosystem functions globally.Spatial PPT–ANPP relationships for grasslands are found remarkably similar around the world,but whether and how they change during periods of extended climatic anomalies remain unknown.Here,we quantifed how regional-scale PPTANPP relationships vary between an extended wet and a dry period by taking advantage of a 35-year record of PPT and NDVI(as a surrogate for ANPP)at 1700 sites in the temperate grasslands of northern China.We found a sharp decrease in the strength of the spatial PPT–ANPP relationship during an 11-year period of below average PPT.We attributed the collapse of this relationship to asynchrony in the responses of different grassland types to this decadal period of increased aridity.Our results challenge the robustness of regional PPT–productivity if aridity in grasslands is increased globally by climate change.展开更多
基金Supported by Danone Institute China Diet Nutrition Research and Communication grant (2006)
文摘AIM:To investigate the differences in cultivable gut bacteria and peroxisome proliferator-activated receptor γ2(PPAR-γ2) gene Pro12Ala variation in obese and normal-weight Chinese people.METHODS:Using culture methods,the amounts of Escherichia coli,Enterococci,Bacteroides,Lactobacilli,Bif idobacteria and Clostridium perfringens(C.perfringens) in the feces of 52 obese participants [body mass index(BMI):≥ 28 kg/m2] and 52 participants of normalweight(BMI:18.5-24 kg/m2) were obtained.Study participants completed comprehensive questionnaires and underwent clinical laboratory tests.The polymerase chain reaction-restriction fragment length polymorphism(PCR-PFLP) assay was used to analyze PPAR-γ2 gene Pro12Ala variation.RESULTS:The obese group exhibited a lower amount of C.perfringens(6.54 ± 0.65 vs 6.94 ± 0.57,P = 0.001)and Bacteroides(9.81 ± 0.58 vs 10.06 ± 0.39,P = 0.012) than their normal-weight counterparts.No major differences were observed in Pro12Ala genotype distribution between the two groups;however,obese individuals with a Pro/Ala genotype had a signif icantly lower level of Bacteroides(9.45 ± 0.62 vs 9.93 ± 0.51,P = 0.027) than those with a Pro/Pro genotype.In addition,the obese group demonstrated a higher stool frequency(U = 975,P < 0.001) and a looser stool(U = 1062,P = 0.015) than the normal-weight group.CONCLUSION:Our results indicated interactions among cultivable gut flora,host genetic factors and obese phenotype and this might be helpful for obesity prevention.
基金funded by the National Natural Science Foundation of China(Grant No.41171084)the Natural Science Foundation of Tibet Autonomous Region(Response of species richness and aboveground biomass to warming in the alpine meadows of Tibet)
文摘Predicting how human activity will influence the response of alpine grasslands to future warming has many uncertainties.In this study, a field experiment with controlled warming and clipping was conducted in an alpine meadow at three elevations（4313 m, 4513 m and 4693 m） in Northern Tibet to test the hypothesis that clipping would alter warming effect on biomass production.Open top chambers（OTCs） were used to increase temperature since July,2008 and the OTCs increased air temperature by approximately 0.9o C ~ 1.8o C during the growing in2012.Clipping was conducted three times one year during growing season and the aboveground parts of all live plants were clipped to approximately 0.01 m in height using scissors since 2009.Gross primary production（GPP） was calculated from the Moderate-Resolution Imaging Spectroradiometer GPP algorithm and aboveground plant production was estimated using the surface-measured normalized difference vegetation index in 2012.Warming decreased the GPP, aboveground biomass（AGB） and aboveground net primary production（ANPP） at all three elevations when clipping was not applied.In contrast, warming increased AGB at all three elevations, GPP at the two lower elevations and ANPP at the two higher elevations when clipping was applied.These findings show that clipping reduced the negative effect of warming on GPP, AGB and ANPP, suggesting that clipping may reduce the effect of climate warming on GPP, AGB and ANPP in alpine meadows on the Tibetan Plateau, and therefore, may be a viable strategy for mitigating the effects of climate change on grazing and animal husbandry on the Tibetan Plateau.
文摘Generally the irreducible water saturation of low permeability gas reservoir is quite high which leads to the permeability stress sensibility and threshold pressure gradient. Under the assumption that permeability varies with experimental law of the pseudo pressure drop, according to concepts of perturbable ellipses and equivalent developing regulations, the calculation method of stable production of hydraulically fractured gas well in low permeability reservoirs is investigated with threshold pressure. And productivity curve is drawn and analyzed. The result shows that, permeability modulus and threshold pressure have effect on production of fractured gas well. The higher the permeability modulus and the threshold pressure, the lower the production is. Therefore, the impact of stress sensitive and threshold pressure must he considered when analyzing the productivity of vertical fracture well in low permeability gas reservoir.
基金supported by the National Natural Science Foundation of China(31922053)the start-up fund of Hainan University(Grant No.KYQD(ZR)21096)the National Key R&D Program of China(2017YFA0604801).
文摘Precipitation(PPT)is the primary climatic determinant of plant growth and aboveground net primary productivity(ANPP)for many of the world’s major terrestrial ecosystems.Thus,relationships between PPT and productivity can provide insight into how changes in climate may alter ecosystem functions globally.Spatial PPT–ANPP relationships for grasslands are found remarkably similar around the world,but whether and how they change during periods of extended climatic anomalies remain unknown.Here,we quantifed how regional-scale PPTANPP relationships vary between an extended wet and a dry period by taking advantage of a 35-year record of PPT and NDVI(as a surrogate for ANPP)at 1700 sites in the temperate grasslands of northern China.We found a sharp decrease in the strength of the spatial PPT–ANPP relationship during an 11-year period of below average PPT.We attributed the collapse of this relationship to asynchrony in the responses of different grassland types to this decadal period of increased aridity.Our results challenge the robustness of regional PPT–productivity if aridity in grasslands is increased globally by climate change.
