植物组培研究者们普遍认为,“非分化”的细胞和愈伤组织培养物可产生体克隆变异。因此,在商业上研究人员依靠生长在固体培养基上的顶芽分生组织或芽外植体培养物来获得一致的植株生长。通过体细胞胚胎发生可再生小植株,这可以在液体培...植物组培研究者们普遍认为,“非分化”的细胞和愈伤组织培养物可产生体克隆变异。因此,在商业上研究人员依靠生长在固体培养基上的顶芽分生组织或芽外植体培养物来获得一致的植株生长。通过体细胞胚胎发生可再生小植株,这可以在液体培养基中进行,避免了很多操作过程,并提高了自动化。但体细胞胚胎发生通常包括一个中间细胞或愈伤的培养阶段,这意味着产生体细胞克隆变异具危险性。University of Geisenheim and Ahrensburg′s Institut fur Gartenbauliche Pflanzenzuchtung的T.Geier和同事们已发现。展开更多
The somaclone, C39, derived by tissue culture from the obligate apomict Paspalum dilatatum cv Raki (2n=50), had 50 chromosomes and a karyotype apparently identical to Raki.SC2 seedlings of C39 showed a high degree of ...The somaclone, C39, derived by tissue culture from the obligate apomict Paspalum dilatatum cv Raki (2n=50), had 50 chromosomes and a karyotype apparently identical to Raki.SC2 seedlings of C39 showed a high degree of phenotypic variation which was of ten associated with increased chromosome numbers, but some of the variant seedlings were karyotypically indistinguishable from Raki or C39. Plants with increased chromosome numbers exhibited a high degree of intraplant chromosome variation (aneusomaty). In one of the SC2seedlings, the chromosome number of root tip cells varied from 58 to 82 and in several other seedlings the range was more than 10. The results suggested that the ability to form seed apomictically was much reduced in C39 and that this plant showed some capacity for sexual reproduction and the resulting seedlings, with a chromosome number of about 70, were genetically unstable. Of 11 SC2 seedlings examined cytolog ically, 6 did not produce any viable seed. Seedlings grown from seed of the remaining 5 plants showed that aneusomaty persisted in the SC3 generation. SC3 seedlings which were phenotypically similar to their maternal parent showed a similar range of chromosome numbers to that parent. Some of the SC3 seedlings exhibited an even wider range of chromosome numbers (e.g.56-136), and these plants were all dwarfs.展开更多
文摘植物组培研究者们普遍认为,“非分化”的细胞和愈伤组织培养物可产生体克隆变异。因此,在商业上研究人员依靠生长在固体培养基上的顶芽分生组织或芽外植体培养物来获得一致的植株生长。通过体细胞胚胎发生可再生小植株,这可以在液体培养基中进行,避免了很多操作过程,并提高了自动化。但体细胞胚胎发生通常包括一个中间细胞或愈伤的培养阶段,这意味着产生体细胞克隆变异具危险性。University of Geisenheim and Ahrensburg′s Institut fur Gartenbauliche Pflanzenzuchtung的T.Geier和同事们已发现。
文摘The somaclone, C39, derived by tissue culture from the obligate apomict Paspalum dilatatum cv Raki (2n=50), had 50 chromosomes and a karyotype apparently identical to Raki.SC2 seedlings of C39 showed a high degree of phenotypic variation which was of ten associated with increased chromosome numbers, but some of the variant seedlings were karyotypically indistinguishable from Raki or C39. Plants with increased chromosome numbers exhibited a high degree of intraplant chromosome variation (aneusomaty). In one of the SC2seedlings, the chromosome number of root tip cells varied from 58 to 82 and in several other seedlings the range was more than 10. The results suggested that the ability to form seed apomictically was much reduced in C39 and that this plant showed some capacity for sexual reproduction and the resulting seedlings, with a chromosome number of about 70, were genetically unstable. Of 11 SC2 seedlings examined cytolog ically, 6 did not produce any viable seed. Seedlings grown from seed of the remaining 5 plants showed that aneusomaty persisted in the SC3 generation. SC3 seedlings which were phenotypically similar to their maternal parent showed a similar range of chromosome numbers to that parent. Some of the SC3 seedlings exhibited an even wider range of chromosome numbers (e.g.56-136), and these plants were all dwarfs.
