Objective: To develop a new high sensitivity, rapid and simple mycobacterium tuberculosis DNA detection method using fluorescence polarization technology. Methods: In our asymmetric PCR protocol, 100 times mole of TB-...Objective: To develop a new high sensitivity, rapid and simple mycobacterium tuberculosis DNA detection method using fluorescence polarization technology. Methods: In our asymmetric PCR protocol, 100 times mole of TB-R primer was added than in the usual symmetric PCR to get enough single strands PCR product. The probe TB-5′-TAMRA and PCR products were mixed in a tube and incubate for 5-15 min at 46 ℃.The polarization (mp) was measured using victor2 Multilabel Plate Reader. Results: Asymmetric and symmetric PCR products were analyzed by the FP method. Asymmetric PCR products are detected more sensitively than symmetric ones. The polarization values of probe associated with asymmetric products were much higher than with symmetric products. Conclusion: This fluorescence polarization assay in conjunction with asymmetric PCR is a powerful and widely applicable method for the rapid and sensitive detection of micro-organisms in clinical laboratories.展开更多
The line intensities of 001-000 transition of the asymptotic asymmetric-top Oa molecule at several temperatures are calculated by directly calculating the partition functions and regarding the rotationless transition ...The line intensities of 001-000 transition of the asymptotic asymmetric-top Oa molecule at several temperatures are calculated by directly calculating the partition functions and regarding the rotationless transition dipole moment squared as a constant. The calculated values of the total internM partition sums （TIPS） are consistent with the data of HITRAN database with -0.61% at 296 K. The calculated line intensity data at 500 K and 3000 K are also in excellent agreement with the data in HITRAN database with less than 0.659% and 5.458% at 500 K and 3000 K, which provide a strong support for the calculations of partition function and fine intensity at high temperature. Then we extend the calculation to higher temperatures. The line intensities and simulated spectra of v3 band of the asymptotic asymmetric-top O3 molecule at 4000 and 5000 K are reported. The results are of significance for the studying of the molecular high-temperature spectrum including experimental measurements and theoretical calculations.展开更多
A detailed procedure for the calculation of the third-harmonic-generation susceptibility tensor is given in special asymmetric quantum wells, and an analytic formula for the third-harmonic-generation susceptibility is...A detailed procedure for the calculation of the third-harmonic-generation susceptibility tensor is given in special asymmetric quantum wells, and an analytic formula for the third-harmonic-generation susceptibility is obtained by the compact density matrix approach and the iterative procedure. Finally, the numerical results are presented for typical GaAs/AlGaAs asymmetric quantum wells. The calculated results show that the origin of the large thirdharmonic-generation susceptibility is due to the increase in asymmetry of the quantum well.展开更多
In a simple hadronie model, the two-photon exchange contributions to the single spin asymmetries for the nucleon and the 3He are estimated. The results show that the elastic contributions of two-photon exchange to the...In a simple hadronie model, the two-photon exchange contributions to the single spin asymmetries for the nucleon and the 3He are estimated. The results show that the elastic contributions of two-photon exchange to the single spin asymmetries for the nucleon are rather small while those for the 3He are relatively large. Besides the strong angular dependence, the two-photon contributions to the single transfer. spin asymmetry for the 3He are very sensitive to the momentum展开更多
Wave group is important in ocean wave theory and applications. In the past, nonlinear interaction among wave groups has been studied on the basis of the nonlinear Sehrrdinger equation. Using this theoretical approach,...Wave group is important in ocean wave theory and applications. In the past, nonlinear interaction among wave groups has been studied on the basis of the nonlinear Sehrrdinger equation. Using this theoretical approach, we found that the nonlinear interaction among wave groups causes asymmetry in the shape of the wave envelope （steeper in the front of the curve of the envelope）. An important consequence of this asymmetry is that the highest wave in a wave group appears one individual wave length ahead of the center of the wave group. Further results show that the degree of envelope asymmetry increases with increasing spectral width and the wave steepness. This theoretical analysis has been supplemented by a systematic experimental study of wind waves. Laboratory and some open sea wave data were analyzed. The results show that the shape of the wind wave envelope of wind waves has the same asymmetry predicted by the theoretical approach. The observed degree of deformation of the envelope also increases with increasing spectral width and the wave steepness as predicted by theory. These conclusions have important ramifications for practical applications of ocean wave theory.展开更多
文摘Objective: To develop a new high sensitivity, rapid and simple mycobacterium tuberculosis DNA detection method using fluorescence polarization technology. Methods: In our asymmetric PCR protocol, 100 times mole of TB-R primer was added than in the usual symmetric PCR to get enough single strands PCR product. The probe TB-5′-TAMRA and PCR products were mixed in a tube and incubate for 5-15 min at 46 ℃.The polarization (mp) was measured using victor2 Multilabel Plate Reader. Results: Asymmetric and symmetric PCR products were analyzed by the FP method. Asymmetric PCR products are detected more sensitively than symmetric ones. The polarization values of probe associated with asymmetric products were much higher than with symmetric products. Conclusion: This fluorescence polarization assay in conjunction with asymmetric PCR is a powerful and widely applicable method for the rapid and sensitive detection of micro-organisms in clinical laboratories.
基金supported by National Natural Science Foundation of China under Grant No.10574096the Major Program for Basic Research of National Security of China under Grant No.5134202-04the Natural Science Foundation of the Bureau of Education of Guizhou Province under Grant No.2006204
文摘The line intensities of 001-000 transition of the asymptotic asymmetric-top Oa molecule at several temperatures are calculated by directly calculating the partition functions and regarding the rotationless transition dipole moment squared as a constant. The calculated values of the total internM partition sums （TIPS） are consistent with the data of HITRAN database with -0.61% at 296 K. The calculated line intensity data at 500 K and 3000 K are also in excellent agreement with the data in HITRAN database with less than 0.659% and 5.458% at 500 K and 3000 K, which provide a strong support for the calculations of partition function and fine intensity at high temperature. Then we extend the calculation to higher temperatures. The line intensities and simulated spectra of v3 band of the asymptotic asymmetric-top O3 molecule at 4000 and 5000 K are reported. The results are of significance for the studying of the molecular high-temperature spectrum including experimental measurements and theoretical calculations.
文摘A detailed procedure for the calculation of the third-harmonic-generation susceptibility tensor is given in special asymmetric quantum wells, and an analytic formula for the third-harmonic-generation susceptibility is obtained by the compact density matrix approach and the iterative procedure. Finally, the numerical results are presented for typical GaAs/AlGaAs asymmetric quantum wells. The calculated results show that the origin of the large thirdharmonic-generation susceptibility is due to the increase in asymmetry of the quantum well.
文摘In a simple hadronie model, the two-photon exchange contributions to the single spin asymmetries for the nucleon and the 3He are estimated. The results show that the elastic contributions of two-photon exchange to the single spin asymmetries for the nucleon are rather small while those for the 3He are relatively large. Besides the strong angular dependence, the two-photon contributions to the single transfer. spin asymmetry for the 3He are very sensitive to the momentum
基金Supported by the National Science Foundation of China (No. 40576007)the New Century Excellent Talent Foundation from Education Ministry of China (No. NCET-08-0509)
文摘Wave group is important in ocean wave theory and applications. In the past, nonlinear interaction among wave groups has been studied on the basis of the nonlinear Sehrrdinger equation. Using this theoretical approach, we found that the nonlinear interaction among wave groups causes asymmetry in the shape of the wave envelope （steeper in the front of the curve of the envelope）. An important consequence of this asymmetry is that the highest wave in a wave group appears one individual wave length ahead of the center of the wave group. Further results show that the degree of envelope asymmetry increases with increasing spectral width and the wave steepness. This theoretical analysis has been supplemented by a systematic experimental study of wind waves. Laboratory and some open sea wave data were analyzed. The results show that the shape of the wind wave envelope of wind waves has the same asymmetry predicted by the theoretical approach. The observed degree of deformation of the envelope also increases with increasing spectral width and the wave steepness as predicted by theory. These conclusions have important ramifications for practical applications of ocean wave theory.
