PLLA and PGLA sutures for decomposable esophageal stent were investigated in phosphate buffer solution （PBS） （pH=7.4） at 37 ℃ for a period of 8 weeks. In vitro degradation was studied by determining the change of...PLLA and PGLA sutures for decomposable esophageal stent were investigated in phosphate buffer solution （PBS） （pH=7.4） at 37 ℃ for a period of 8 weeks. In vitro degradation was studied by determining the change of weight loss, pH value, intrinsic viscosity, tensile strength, orientation degree, degree of crystallinity, melting point and surface morphology of the suture samples. The results showed that all properties of PLLA sutures had no obvious changes, however, the properties of PGLA sutures all changed significantly. The pH value, intrinsic viscosity, tensile strength, orientation degree and degree of crystallinity decreased gradually, and the weight loss of PGLA sutures increased with the degradation time. At 6th week, tensile strength of PGLA sutures nearly reached O, and weight loss approached to 70% at 8th week. The results of DSC showed that melting point of crystalline region of PGLA sutures substantially remained unchanged and melting heat enthalpy increased gradually during in vitro degradation, and the new ordered regions appeared in the amorphous area. The results of SEM showed that surface coating of PGLA sutures spalled initially, and then the sutures occured transverse rupture. Therefore, PGLA suture is suitable to prepare decomposable esophageal stent to expand benign esophageal stenosis or stricture, but stent prepared by PLLA suture is not appropriate for the treatment of benign esophageal stenosis because it is decomposed for more than 2 months.展开更多
Objective： To assess local effect of celecoxib on nerve regeneration in a rat sciatic nerve transec- tion model. Methods： Forty-five male healthy white Wistar rats were randomly divided into three experimental grou...Objective： To assess local effect of celecoxib on nerve regeneration in a rat sciatic nerve transec- tion model. Methods： Forty-five male healthy white Wistar rats were randomly divided into three experimental groups （n= 15 for each）： sham-operation （SHAM）, control （SIL） and celecoxib treated （SIL/CLX） groups. In SHAM group after anesthesia left sciatic nerve was exposed and after homeo- stasis muscle was sutured. In SIL group the left sciatic nerve was exposed in the same way and transected proximal to tibioperoneal bifurcation leaving a 10 mm gap. Proximal and distal stumps were each inserted into a silicone tube and filled with 10 gl phosphate buffered solution. In SIL/CLX group defect was bridged using a silicone tube filled with 10 μl celecoxib （0.1 g/L）. Results： Functional study and gastrocnemius muscle mass confirmed faster and better recovery of regenerated axons in SIL/CLX than in SIL group （P〈0.05）. Morphometric indices of regenerated fibers showed number and diameter of the myelinated fibers in SIL/CLX were significantly greater than those in control group. In immunohistochemistry, lo- cation of reactions to S-100 in SIL/CLX was clearly more positive than that in SIL group. Conclusion： Response to local treatment ofcelecoxib demonstrates that it influences and improves functional re- covery of peripheral nerve regeneration.展开更多
文摘目的:通过对比研究两种冲管溶液对预防鼻肠管堵塞的效果。方法:本研究遵循随机抽样原则,将留置鼻肠管的80例ICU患者随机分为对照组和观察组,对照组在肠内营养结束时,利用脉冲式将20 mL的温开水注入管腔内并留夹,同时需注意鼻肠管腔内的冲洗液无需弃掉;观察组则以温开水冲管后注入20 mL 5%碳酸氢钠并夹管,将碳酸氢钠保留于鼻肠管腔内。观察比较2组患者留置鼻肠管期间堵管的发生率。结果:观察组患者鼻肠管堵塞发生率低于对照组,差异具有统计学意义(P <0.05)。结论:在常规温开水冲管后,利用5%碳酸氢钠注入管腔,可降低鼻肠管堵塞发生率。
基金Shandong Provincial Science and Technology Development Program grant number: 2009GG10002077
文摘PLLA and PGLA sutures for decomposable esophageal stent were investigated in phosphate buffer solution （PBS） （pH=7.4） at 37 ℃ for a period of 8 weeks. In vitro degradation was studied by determining the change of weight loss, pH value, intrinsic viscosity, tensile strength, orientation degree, degree of crystallinity, melting point and surface morphology of the suture samples. The results showed that all properties of PLLA sutures had no obvious changes, however, the properties of PGLA sutures all changed significantly. The pH value, intrinsic viscosity, tensile strength, orientation degree and degree of crystallinity decreased gradually, and the weight loss of PGLA sutures increased with the degradation time. At 6th week, tensile strength of PGLA sutures nearly reached O, and weight loss approached to 70% at 8th week. The results of DSC showed that melting point of crystalline region of PGLA sutures substantially remained unchanged and melting heat enthalpy increased gradually during in vitro degradation, and the new ordered regions appeared in the amorphous area. The results of SEM showed that surface coating of PGLA sutures spalled initially, and then the sutures occured transverse rupture. Therefore, PGLA suture is suitable to prepare decomposable esophageal stent to expand benign esophageal stenosis or stricture, but stent prepared by PLLA suture is not appropriate for the treatment of benign esophageal stenosis because it is decomposed for more than 2 months.
文摘Objective： To assess local effect of celecoxib on nerve regeneration in a rat sciatic nerve transec- tion model. Methods： Forty-five male healthy white Wistar rats were randomly divided into three experimental groups （n= 15 for each）： sham-operation （SHAM）, control （SIL） and celecoxib treated （SIL/CLX） groups. In SHAM group after anesthesia left sciatic nerve was exposed and after homeo- stasis muscle was sutured. In SIL group the left sciatic nerve was exposed in the same way and transected proximal to tibioperoneal bifurcation leaving a 10 mm gap. Proximal and distal stumps were each inserted into a silicone tube and filled with 10 gl phosphate buffered solution. In SIL/CLX group defect was bridged using a silicone tube filled with 10 μl celecoxib （0.1 g/L）. Results： Functional study and gastrocnemius muscle mass confirmed faster and better recovery of regenerated axons in SIL/CLX than in SIL group （P〈0.05）. Morphometric indices of regenerated fibers showed number and diameter of the myelinated fibers in SIL/CLX were significantly greater than those in control group. In immunohistochemistry, lo- cation of reactions to S-100 in SIL/CLX was clearly more positive than that in SIL group. Conclusion： Response to local treatment ofcelecoxib demonstrates that it influences and improves functional re- covery of peripheral nerve regeneration.
