AIM:To investigate the role of FAT10 and mutant p53 in the pathogenesis,severity and prognosis of gastric cancer.METHODS:FAT10,mutant p53 mRNA and protein levels were measured by reverse transcription(RT)-PCR and immu...AIM:To investigate the role of FAT10 and mutant p53 in the pathogenesis,severity and prognosis of gastric cancer.METHODS:FAT10,mutant p53 mRNA and protein levels were measured by reverse transcription(RT)-PCR and immunohistochemistry in gastric cancer tissue(n = 62),tumoradjacent tissue(n = 62) and normal gastric tissue(n = 62).Relation of FAT10 and mutant p53 expression with clinicopathological features and clinical outcomes of gastric cancer patients were analyzed.RESULTS:The FAT10,mutant p53 mRNA and protein levels were signif icantly higher in gastric cancer than in its adjacent and normal tissue.The FAT10 and mutant p53 levels in gastric cancer tissue were significantly correlated with lymph node metastasis and tumor,nodes,metastasis(TNM) staging.Moreover,the high FAT10 level was associated with the overall survival rate of patients.Multivariate Cox-proportional hazards model analysis showed that mRNA and protein levels of FAT10 and mutant p53,lymph node metastasis,distant metastasis and TNM stage were the independent prognostic factors for gastric cancer.CONCLUSION:FAT10 may be involved in gastric carcinogenesis,and is a potential marker for the prognosis of gastric cancer patients.FAT10 and mutant p53 may play a common role in the carcinogenesis of gastric cancer.展开更多
Crowded scene analysis is currently a hot and challenging topic in computer vision field. The ability to analyze motion patterns from videos is a difficult, but critical part of this problem. In this paper, we propose...Crowded scene analysis is currently a hot and challenging topic in computer vision field. The ability to analyze motion patterns from videos is a difficult, but critical part of this problem. In this paper, we propose a novel approach for the analysis of motion patterns by clustering the tracklets using an unsupervised hierarchical clustering algorithm, where the similarity between tracklets is measured by the Longest Common Subsequences. The tracklets are obtained by tracking dense points under three effective rules, therefore enabling it to capture the motion patterns in crowded scenes. The analysis of motion patterns is implemented in a completely unsupervised way, and the tracklets are clustered automatically through hierarchical clustering algorithm based on a graphic model. To validate the performance of our approach, we conducted experimental evaluations on two datasets. The results reveal the precise distributions of motion patterns in current crowded videos and demonstrate the effectiveness of our approach.展开更多
Pituitary adenylate cyclase activating polypeptide （PACAP） has a wide range of biological functions. We cloned the full-length cDNAs encoding PACAP and PACAP-related peptide （PRP） from the brain of largemouth bass...Pituitary adenylate cyclase activating polypeptide （PACAP） has a wide range of biological functions. We cloned the full-length cDNAs encoding PACAP and PACAP-related peptide （PRP） from the brain of largemouth bass （Micropterus salmoides） and used real-time quantitative PCR to detect PRP- PACAP mRNA expression. The PRP-PACAP cDNA has two variants expressed via alternative splicing： a long form, which encodes both PRP and PACAP, and a short form, which encodes only PACAR Sequence analysis results are consistent with a higher conservation of PACAP than PRP peptide sequences. The expression of PACAP-Iong and PACAP-short transcripts was highest in the forebrain, followed by the medulla, midbrain, pituitary, stomach, cerebellum, intestine, and kidney; however, these transcripts were either absent or were weakly expressed in the muscle, spleen, gill, heart, fatty tissue, and liver. The level of PACAP-short transcript expression was significantly higher than expression of the long transcript in the forebrain, cerebella, pituitary and intestine, but lower than that of the long transcript in the stomach. PA CAP- long and PACAP-short transcripts were first detected at the blastula stage of embryogenesis, and the level of expression increased markedly between the muscular contraction stage and 3 d post hatch （dph）. The expression of PACAP-long and PACAP-short transcripts decreased significantly in the brain following 4 d fasting compared with the control diet group. The down-regulation effect was enhanced as fasting continued. Conversely, expression levels increased significantly after 3 d of re-feeding. Our results suggest that PRP- PA CAP acts as an important factor in appetite regulation in largemouth bass.展开更多
Objective: The aim of our study was to investigate the expression of Survivin in lung adenocarcinoma of Xuanwei and Kunming patients. Methods: Twenty-five specimens of Xuanwei patients and 28 specimens of Kunming pati...Objective: The aim of our study was to investigate the expression of Survivin in lung adenocarcinoma of Xuanwei and Kunming patients. Methods: Twenty-five specimens of Xuanwei patients and 28 specimens of Kunming patients were observed and analyzed. The Survivin expression was detected by immunohistochemistry. The results were quantitatively analyzed by image analysis system. Results: There were significant differences in Survivin expression (P < 0.01) between Xuanwei patients and Kunming patients. Conclusion: Survivin expression in lung adenocarcinoma of Xuanwei patients was significantly higher than that of Kunming patients. The pathogenesis of lung adenocarcinoma might be different between Xuanwei patients and Kunming patients. High Survivin expression might be one of the reasons to explain high incidence of lung cancer in Xuanwei.展开更多
In view of the importance of the hotel segment for the tourism and for the economy of countries such as Portugal, the objective of this study was to measure the level of orientation for the market of the largest hotel...In view of the importance of the hotel segment for the tourism and for the economy of countries such as Portugal, the objective of this study was to measure the level of orientation for the market of the largest hotel groups of Portugal. This investigation initially emphasized the importance of the marketing for the organizations, mainly the orientation for the market. After a brief explanation on the hotel segment in Portugal, an empirical study was presented, of quantitative, exploratory and traversal character, performed with the largest groups of 20 hotels of Portugal, using as an instrument of collection data, the traditional Markor scale （market orientation） adapted to the hotel sector. After analyzing the data, it was found in the investigated organizations that a good capacity to generate market information and response to the market, which formed two of the three constructs of Markor scale. However the results obtained with the construct of the dissemination of market information were below the expectations. It was possible to conclude that marketing professionals of the large hotel groups in Portugal are well oriented to the market, something not shared by other investigated departments of the hotels. So the current challenge for the main networks of hotels in Portugal is to improve the internal dissemination of information that marketing professionals gathered at the market.展开更多
为明确植物常用颜色测定工具英国皇家园艺学会比色卡(Royal Horticultural Society Color Chart,RHSCC)的颜色特征及其在花色测定应用上的科学性,以884个RHSCC色号和150个盆栽香石竹品种花色为研究对象,采用色差仪测定法和色卡目测比对...为明确植物常用颜色测定工具英国皇家园艺学会比色卡(Royal Horticultural Society Color Chart,RHSCC)的颜色特征及其在花色测定应用上的科学性,以884个RHSCC色号和150个盆栽香石竹品种花色为研究对象,采用色差仪测定法和色卡目测比对法进行颜色测定,利用统计学方法和色差公式进行颜色分析。结果表明:RHSCC 884个色号在高明度、红色及黄色分量区分布密集;在a^(*)b^(*)二维分布上集中于第I和第Ⅱ象限;60个色号两两间最小色差值为0.50~1.50,其中4个色号处于不同UPOV(国际植物新品种保护联盟)色组。RHSCC目测法结果显示,150个盆栽香石竹品种中,47个品种花色难以比对到准确的RHSCC色号(花色及其近似色卡色差值>6.00);部分品种花色可比对到2个RHSCC色号(色卡间色差值<3.00)。RHSCC目测法对品种分组的结果表明,粉色系颜色区间过大,白色和深紫色系区间过小;黄绿色、黄色和绿色3个色系难以在颜色阈值上区分;多数色系含有游离颜色等问题。综上,RHSCC在花色测定时需要考虑其局限性,包括部分色号间色差值过小,人眼分辨困难;部分颜色未覆盖,导致观测结果不准确;比对结果难以有效用于品种分组等。展开更多
OBJECTIVE:To investigate the relationship of electro-parameters and the electroacupuncture sensation(EAS),which is thought to be an important factor for optimal treatment.METHODS:The frequency steps and compositions o...OBJECTIVE:To investigate the relationship of electro-parameters and the electroacupuncture sensation(EAS),which is thought to be an important factor for optimal treatment.METHODS:The frequency steps and compositions of three frequently used electrical stimulations were set when the switch of the electroacupuncture apparatus was turned to the second or third grade of the dense-disperse frequency wave(DD2and DD3,respectively) or the second grade of the continuous wave(C2).Three groups of patients according to the three electroacupuncture stimulations were divided again into three sub-groups according to the stimulated acupoints:the face acupoint Quanliao(SI 18),the upper-limb acupoint Quchi(LI 11) and the back acupoint Dachangshu(BL25).The EAS values were measured every 5 min during 30 min electroacupuncture treatments using a visual analogue scale.RESULTS:The frequency compositions of the three electroacupuncture stimulations were 3.3 and 33 Hz,12.5 and 66.7 Hz,and 3.3 and 3.3 Hz;each frequency step was 30,54 and 0 Hz,respectively.In each sub-group of the C2 group,the EAS values from 10 to 30 min were significantly weaker than at 0 min.The sensation fluctuations in the DD2 and DD3 groups were different during the 30 min.CONCLUSION:The greater the frequency step of the electroacupuncture stimulation,the longer the needling sensation lasted.The electroacupuncture stimulations of the DD3 group were unsuitable for the facial acupoint because of its painful and uncomfortable EAS,but more suitable for the back acupoint.展开更多
文摘AIM:To investigate the role of FAT10 and mutant p53 in the pathogenesis,severity and prognosis of gastric cancer.METHODS:FAT10,mutant p53 mRNA and protein levels were measured by reverse transcription(RT)-PCR and immunohistochemistry in gastric cancer tissue(n = 62),tumoradjacent tissue(n = 62) and normal gastric tissue(n = 62).Relation of FAT10 and mutant p53 expression with clinicopathological features and clinical outcomes of gastric cancer patients were analyzed.RESULTS:The FAT10,mutant p53 mRNA and protein levels were signif icantly higher in gastric cancer than in its adjacent and normal tissue.The FAT10 and mutant p53 levels in gastric cancer tissue were significantly correlated with lymph node metastasis and tumor,nodes,metastasis(TNM) staging.Moreover,the high FAT10 level was associated with the overall survival rate of patients.Multivariate Cox-proportional hazards model analysis showed that mRNA and protein levels of FAT10 and mutant p53,lymph node metastasis,distant metastasis and TNM stage were the independent prognostic factors for gastric cancer.CONCLUSION:FAT10 may be involved in gastric carcinogenesis,and is a potential marker for the prognosis of gastric cancer patients.FAT10 and mutant p53 may play a common role in the carcinogenesis of gastric cancer.
基金supported in part by National Basic Research Program of China (973 Program) under Grant No. 2011CB302203the National Natural Science Foundation of China under Grant No. 61273285
文摘Crowded scene analysis is currently a hot and challenging topic in computer vision field. The ability to analyze motion patterns from videos is a difficult, but critical part of this problem. In this paper, we propose a novel approach for the analysis of motion patterns by clustering the tracklets using an unsupervised hierarchical clustering algorithm, where the similarity between tracklets is measured by the Longest Common Subsequences. The tracklets are obtained by tracking dense points under three effective rules, therefore enabling it to capture the motion patterns in crowded scenes. The analysis of motion patterns is implemented in a completely unsupervised way, and the tracklets are clustered automatically through hierarchical clustering algorithm based on a graphic model. To validate the performance of our approach, we conducted experimental evaluations on two datasets. The results reveal the precise distributions of motion patterns in current crowded videos and demonstrate the effectiveness of our approach.
基金Supported by the National Natural Science Foundation of China(No.31201985)the National Key Technology R&D Program of China(No.2012BAD26B03)
文摘Pituitary adenylate cyclase activating polypeptide （PACAP） has a wide range of biological functions. We cloned the full-length cDNAs encoding PACAP and PACAP-related peptide （PRP） from the brain of largemouth bass （Micropterus salmoides） and used real-time quantitative PCR to detect PRP- PACAP mRNA expression. The PRP-PACAP cDNA has two variants expressed via alternative splicing： a long form, which encodes both PRP and PACAP, and a short form, which encodes only PACAR Sequence analysis results are consistent with a higher conservation of PACAP than PRP peptide sequences. The expression of PACAP-Iong and PACAP-short transcripts was highest in the forebrain, followed by the medulla, midbrain, pituitary, stomach, cerebellum, intestine, and kidney; however, these transcripts were either absent or were weakly expressed in the muscle, spleen, gill, heart, fatty tissue, and liver. The level of PACAP-short transcript expression was significantly higher than expression of the long transcript in the forebrain, cerebella, pituitary and intestine, but lower than that of the long transcript in the stomach. PA CAP- long and PACAP-short transcripts were first detected at the blastula stage of embryogenesis, and the level of expression increased markedly between the muscular contraction stage and 3 d post hatch （dph）. The expression of PACAP-long and PACAP-short transcripts decreased significantly in the brain following 4 d fasting compared with the control diet group. The down-regulation effect was enhanced as fasting continued. Conversely, expression levels increased significantly after 3 d of re-feeding. Our results suggest that PRP- PA CAP acts as an important factor in appetite regulation in largemouth bass.
基金Supported by grants from Basic Project of Social Development and Science Practice Plan of Yunnan Provincial Department of Science (No. 2009ZC120M)Practice Fund of Yunnan Provincial Education Department (No. 09Y0173)
文摘Objective: The aim of our study was to investigate the expression of Survivin in lung adenocarcinoma of Xuanwei and Kunming patients. Methods: Twenty-five specimens of Xuanwei patients and 28 specimens of Kunming patients were observed and analyzed. The Survivin expression was detected by immunohistochemistry. The results were quantitatively analyzed by image analysis system. Results: There were significant differences in Survivin expression (P < 0.01) between Xuanwei patients and Kunming patients. Conclusion: Survivin expression in lung adenocarcinoma of Xuanwei patients was significantly higher than that of Kunming patients. The pathogenesis of lung adenocarcinoma might be different between Xuanwei patients and Kunming patients. High Survivin expression might be one of the reasons to explain high incidence of lung cancer in Xuanwei.
文摘In view of the importance of the hotel segment for the tourism and for the economy of countries such as Portugal, the objective of this study was to measure the level of orientation for the market of the largest hotel groups of Portugal. This investigation initially emphasized the importance of the marketing for the organizations, mainly the orientation for the market. After a brief explanation on the hotel segment in Portugal, an empirical study was presented, of quantitative, exploratory and traversal character, performed with the largest groups of 20 hotels of Portugal, using as an instrument of collection data, the traditional Markor scale （market orientation） adapted to the hotel sector. After analyzing the data, it was found in the investigated organizations that a good capacity to generate market information and response to the market, which formed two of the three constructs of Markor scale. However the results obtained with the construct of the dissemination of market information were below the expectations. It was possible to conclude that marketing professionals of the large hotel groups in Portugal are well oriented to the market, something not shared by other investigated departments of the hotels. So the current challenge for the main networks of hotels in Portugal is to improve the internal dissemination of information that marketing professionals gathered at the market.
文摘为明确植物常用颜色测定工具英国皇家园艺学会比色卡(Royal Horticultural Society Color Chart,RHSCC)的颜色特征及其在花色测定应用上的科学性,以884个RHSCC色号和150个盆栽香石竹品种花色为研究对象,采用色差仪测定法和色卡目测比对法进行颜色测定,利用统计学方法和色差公式进行颜色分析。结果表明:RHSCC 884个色号在高明度、红色及黄色分量区分布密集;在a^(*)b^(*)二维分布上集中于第I和第Ⅱ象限;60个色号两两间最小色差值为0.50~1.50,其中4个色号处于不同UPOV(国际植物新品种保护联盟)色组。RHSCC目测法结果显示,150个盆栽香石竹品种中,47个品种花色难以比对到准确的RHSCC色号(花色及其近似色卡色差值>6.00);部分品种花色可比对到2个RHSCC色号(色卡间色差值<3.00)。RHSCC目测法对品种分组的结果表明,粉色系颜色区间过大,白色和深紫色系区间过小;黄绿色、黄色和绿色3个色系难以在颜色阈值上区分;多数色系含有游离颜色等问题。综上,RHSCC在花色测定时需要考虑其局限性,包括部分色号间色差值过小,人眼分辨困难;部分颜色未覆盖,导致观测结果不准确;比对结果难以有效用于品种分组等。
基金Supported by National Natural Science Fund of China(Research on the Muscle Cell IGF1/PI3K/AKT Pathway Mechanism of Electroacupuncture Treatment for Muscular Atrophy of TCM Flaccidity(No.81373733)Autonomous Momentous Project of the Fujian Institute of Traditional Chinese Medicine(No.2012fjzyyk-1)
文摘OBJECTIVE:To investigate the relationship of electro-parameters and the electroacupuncture sensation(EAS),which is thought to be an important factor for optimal treatment.METHODS:The frequency steps and compositions of three frequently used electrical stimulations were set when the switch of the electroacupuncture apparatus was turned to the second or third grade of the dense-disperse frequency wave(DD2and DD3,respectively) or the second grade of the continuous wave(C2).Three groups of patients according to the three electroacupuncture stimulations were divided again into three sub-groups according to the stimulated acupoints:the face acupoint Quanliao(SI 18),the upper-limb acupoint Quchi(LI 11) and the back acupoint Dachangshu(BL25).The EAS values were measured every 5 min during 30 min electroacupuncture treatments using a visual analogue scale.RESULTS:The frequency compositions of the three electroacupuncture stimulations were 3.3 and 33 Hz,12.5 and 66.7 Hz,and 3.3 and 3.3 Hz;each frequency step was 30,54 and 0 Hz,respectively.In each sub-group of the C2 group,the EAS values from 10 to 30 min were significantly weaker than at 0 min.The sensation fluctuations in the DD2 and DD3 groups were different during the 30 min.CONCLUSION:The greater the frequency step of the electroacupuncture stimulation,the longer the needling sensation lasted.The electroacupuncture stimulations of the DD3 group were unsuitable for the facial acupoint because of its painful and uncomfortable EAS,but more suitable for the back acupoint.
