A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron micr...A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fou-rier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform si-multaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction seg-mentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm.展开更多
Objective： The aim of our study was to determine an optimal slice thickness that was efficient in revealing bronchial imageology of pulmonary nodules （PNs） on multi-slice spiral computed tomography （MSCT） images ...Objective： The aim of our study was to determine an optimal slice thickness that was efficient in revealing bronchial imageology of pulmonary nodules （PNs） on multi-slice spiral computed tomography （MSCT） images preliminarily. Methods： Fifty-four patients with 62 PNs （diameter 〈 3 cm） underwent multidetector-row computed tomography of the chest in a single-breath-hold technique. The raw data were acquired with a collimation of 0.625 mm. Three sets of contiguous im- ages were reconstructed with 1-, 2-, and 5-mm slice thickness, respectively. Bronchial imageology of SPNs on the CT images presented in 1-, 2-, and 5-mm slice thickness was compared. Using the 1-mm sections as the gold standard, an optimal slice thickness in revealing bronchial imageology of PNs was determined. Results： Bronchial imageology of PNs on the CT images presented in 1 mm slice thickness involved 85 bronchi （35 second-fourth generation bronchi; 50 fifth-eighth generation bronchi）. Bronchial imageology on 2-mm-thick sections was as same as that on I-mm-thick sections in 34 second- fourth generation bronchi. No statistically significant difference in number of second- fourth generation bronchi with same bronchial imageology was found between that on 2-mm-thick images and I-mm-thick images （P = 0.836 〉 0.05）. Bronchial imageology on 5-mm-thick sections was as same as that on 1-mm-thick sections in 24 second-fourth generation bronchi. There was statistically significant difference in number of second-fourth generation bronchi with same bronchial imageology between that on 5-mm-thick images and 1-mm-thick images （P = 0.026 〈 0.05）. Bronchial imageology on 2-mm-thick sections was as same as that on 1-mm-thick sections in 38 fifth-eighth generation bronchi. There was statistically significant difference in number of fifth-eighth generation bronchi with same bronchial imageology between that on 2-mm-thick images and 1-mm- thick images （P = 0.029 〈 0.05）. Bronchial imageology on 5-mm-thick images was as same as that on I-mm-thick images in 31 fifth-eighth generation bronchi. There was statistically significant difference in number of fifth-eighth generation bronchi with same bronchial imageology between that on 5-mm-thick sections and I-mm-thick sections （P = 0.001 〈 0.05）. Conclusion： It is suggested that the use of 2-mm slice thickness is suitable in revealing second- fourth generation bronchi and the use of l-mm slice thickness is suitable in revealing fifth-eighth generation bronchi.展开更多
文摘A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fou-rier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform si-multaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction seg-mentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm.
文摘Objective： The aim of our study was to determine an optimal slice thickness that was efficient in revealing bronchial imageology of pulmonary nodules （PNs） on multi-slice spiral computed tomography （MSCT） images preliminarily. Methods： Fifty-four patients with 62 PNs （diameter 〈 3 cm） underwent multidetector-row computed tomography of the chest in a single-breath-hold technique. The raw data were acquired with a collimation of 0.625 mm. Three sets of contiguous im- ages were reconstructed with 1-, 2-, and 5-mm slice thickness, respectively. Bronchial imageology of SPNs on the CT images presented in 1-, 2-, and 5-mm slice thickness was compared. Using the 1-mm sections as the gold standard, an optimal slice thickness in revealing bronchial imageology of PNs was determined. Results： Bronchial imageology of PNs on the CT images presented in 1 mm slice thickness involved 85 bronchi （35 second-fourth generation bronchi; 50 fifth-eighth generation bronchi）. Bronchial imageology on 2-mm-thick sections was as same as that on I-mm-thick sections in 34 second- fourth generation bronchi. No statistically significant difference in number of second- fourth generation bronchi with same bronchial imageology was found between that on 2-mm-thick images and I-mm-thick images （P = 0.836 〉 0.05）. Bronchial imageology on 5-mm-thick sections was as same as that on 1-mm-thick sections in 24 second-fourth generation bronchi. There was statistically significant difference in number of second-fourth generation bronchi with same bronchial imageology between that on 5-mm-thick images and 1-mm-thick images （P = 0.026 〈 0.05）. Bronchial imageology on 2-mm-thick sections was as same as that on 1-mm-thick sections in 38 fifth-eighth generation bronchi. There was statistically significant difference in number of fifth-eighth generation bronchi with same bronchial imageology between that on 2-mm-thick images and 1-mm- thick images （P = 0.029 〈 0.05）. Bronchial imageology on 5-mm-thick images was as same as that on I-mm-thick images in 31 fifth-eighth generation bronchi. There was statistically significant difference in number of fifth-eighth generation bronchi with same bronchial imageology between that on 5-mm-thick sections and I-mm-thick sections （P = 0.001 〈 0.05）. Conclusion： It is suggested that the use of 2-mm slice thickness is suitable in revealing second- fourth generation bronchi and the use of l-mm slice thickness is suitable in revealing fifth-eighth generation bronchi.
